2025-08-01
ENA first public
ERC000025
ENA-CHECKLIST
Thuenen-Institute of Biodiversity
INSDC center name
public
INSDC status
ITS2.lg
Marker
GCTATGCGCGAGCtgcATGCGATACTTGGTGTGAAT
PrimerSeq_FWD_1
NA
PrimerSeq_FWD_2
GCGAGCTGCGCTAtgcGACGCTTCTCCAGACTACAAT
PrimerSeq_REV_1
NA
PrimerSeq_REV_2
ERS20883851
SRA accession
F-HEAD-2-R-TAIL-3
SampleID_ASV
004
SampleName
F-HEAD-2-R-TAIL-3.ITS2_lg
Submitter Id
TGAGTCC
TagFWD
GTCGCCA
TagREV
FWD: TGAGTCC REV: GTCGCCA
adapters
ENVO:01000245
broad-scale environmental context
2020-10-09
collection date
Metabarcoding of nuclear ITS2 long fragment from eDNA extracted from debris in nesting aids deployed on agricultural landscapes in Germany
description
ENVO:02000004
environmental medium
Germany
geographic location (country and/or sea)
52.64385901
DD
geographic location (latitude)
11.25061800
DD
geographic location (longitude)
52.643859009804814
lat
nest tube of cavity-nesting hymenoptera
local environmental context
11.250618000634292
lon
FWD: TGAGTCC REV: GTCGCCA
multiplex identifiers
PCR
nucleic acid amplification
Machery-Nagel NucleoSpin 96 Soil Kit
nucleic acid extraction
insect work metagenome
organism
FWD: GCTATGCGCGAGCtgcATGCGATACTTGGTGTGAAT REV: GCGAGCTGCGCTAtgcGACGCTTCTCCAGACTACAAT
pcr primers
PilotNA
project name
2020-10-09
removal_date
insect work metagenome
scientific_name
Jemmeritz
site
JE
site_ID
1_004_R2S2
source material identifiers
ITS2
target gene
barcoding region
target subfragment
Jemmeritz 1_004_R2S2 ITS2 metabarcoding
title
1_004_R2S2
trap_ID