2025-08-01
ENA first public
ERC000025
ENA-CHECKLIST
Thuenen-Institute of Biodiversity
INSDC center name
public
INSDC status
ERS20824243
SRA accession
F-HEAD-4-R-TAIL-11.ITS2_lg
Submitter Id
FWD: CACTTAT REV: CAATCAG
adapters
ENVO:01000245
broad-scale environmental context
2020-10-09
collection date
Metabarcoding of nuclear ITS2 long fragment from eDNA extracted from debris in nesting aids deployed on agricultural landscapes in Germany
description
ENVO:02000004
environmental medium
Germany
geographic location (country and/or sea)
52.65251701
DD
geographic location (latitude)
11.23856800
DD
geographic location (longitude)
nest tube of cavity-nesting hymenoptera
local environmental context
FWD: CACTTAT REV: CAATCAG
multiplex identifiers
PCR
nucleic acid amplification
Machery-Nagel NucleoSpin 96 Soil Kit
nucleic acid extraction
insect work metagenome
organism
FWD: GCTATGCGCGAGCtgcATGCGATACTTGGTGTGAAT REV: GCGAGCTGCGCTAtgcGACGCTTCTCCAGACTACAAT
pcr primers
PilotNA
project name
insect work metagenome
scientific_name
1_001_R4S2
source material identifiers
ITS2
target gene
barcoding region
target subfragment
Jemmeritz 1_001_R4S2 ITS2 metabarcoding
title