2025-08-01
ENA first public
ERC000011
ENA-CHECKLIST
Department of Artificial Intelligence Medicine, Chiba University Graduate School of Medicine
INSDC center name
public
INSDC status
ERS18821249
SRA accession
E-MTAB-13992:P1
Submitter Id
ArrayExpress
broker name
iPSC derived cell line
cell_line
epithelial cell
cell_type
not collected
collection date
human
common name
Protocols: Sorted hiPSCs were plated onto iMatrix511silk-coated (0.5 μg/cm2) cell culture inserts. The cells were cultured in OEM (DMEM/F12 containing 10 μM Y-27632, 2% B27 supplement, 1% penicillin-streptomycin solution, and 10ng/m of KGF) at 37 °C until confluence (4 weeks).  Total RNA was purified using an RNeasy Micro Kit with RNase-free DNase (both from Qiagen, Hilden, Germany). RNA-seq libraries were prepared using the TruSeq Stranded mRNA Library Prep Kit (Illumina), and the quality and quantity of the amplicons were assessed at all steps through capillary electrophoresis (Agilent Bioanalyzer and TapeStation; Agilent, Santa Clara, CA, USA).
description
10 weeks
developmental stage
normal
disease
EGFP (PAX6) -
fraction
wild type genotype
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
eye
organism part
Homo sapiens
scientific_name
P1
title