2025-08-01
ENA first public
ERC000025
ENA-CHECKLIST
Thuenen-Institute of Biodiversity
INSDC center name
public
INSDC status
QIA
Marker
GCTATGCGCGAGCtgcGGDACWGGWTGAACWGTWTAYCCHCC
PrimerSeq_FWD_1
GCTATGCGCGAGCtgcATGCGATACTTGGTGTGAAT
PrimerSeq_FWD_2
GCGAGCTGCGCTAtgcTAAACTTCAGGGTGACCAAAAAATCA
PrimerSeq_REV_1
GCGAGCTGCGCTAtgcGACGCTTCTCCAGACTACAAT
PrimerSeq_REV_2
ERS20884870
SRA accession
F-HEAD-6-R-TAIL-6
SampleID_ASV
098
SampleName
F-HEAD-6-R-TAIL-6.QIA.ITS2_lg
Submitter Id
TGATACG
TagFWD
TACGCGA
TagREV
FWD: TGATACG REV: TACGCGA
adapters
ENVO:01000245
broad-scale environmental context
2020-10-07
collection date
Metabarcoding of nuclear ITS2 long fragment with multiplex-PCR from eDNA extracted from debris from nesting aids deployed on agricultural landscapes in Germany. Simultaneous amplification of mtCOI and ITS2 fragments.
description
ENVO:02000004
environmental medium
Germany
geographic location (country and/or sea)
51.48358309
DD
geographic location (latitude)
12.15937001
DD
geographic location (longitude)
51.48358309094232
lat
nest tube of cavity-nesting hymenoptera
local environmental context
12.159370007359609
lon
FWD: TGATACG REV: TACGCGA
multiplex identifiers
PCR
nucleic acid amplification
Machery-Nagel NucleoSpin 96 Soil Kit
nucleic acid extraction
insect work metagenome
organism
FWD: GCTATGCGCGAGCtgcATGCGATACTTGGTGTGAAT REV: GCGAGCTGCGCTAtgcGACGCTTCTCCAGACTACAAT
pcr primers
PilotNA
project name
2020-10-07
removal_date
insect work metagenome
scientific_name
Bageritz
site
BAG
site_ID
9_098_R1S2
source material identifiers
ITS2
target gene
barcoding region
target subfragment
Bageritz 9_098_R1S2 ITS2 metabarcoding from multiplexed PCR, Qiagen kit
title
9_098_R1S2
trap_ID