2025-08-01
ENA first public
ERC000025
ENA-CHECKLIST
Thuenen-Institute of Biodiversity
INSDC center name
public
INSDC status
QIA
Marker
GCTATGCGCGAGCtgcGGDACWGGWTGAACWGTWTAYCCHCC
PrimerSeq_FWD_1
GCTATGCGCGAGCtgcATGCGATACTTGGTGTGAAT
PrimerSeq_FWD_2
GCGAGCTGCGCTAtgcTAAACTTCAGGGTGACCAAAAAATCA
PrimerSeq_REV_1
GCGAGCTGCGCTAtgcGACGCTTCTCCAGACTACAAT
PrimerSeq_REV_2
ERS20885015
SRA accession
F-HEAD-10-R-TAIL-3
SampleID_ASV
113
SampleName
F-HEAD-10-R-TAIL-3.QIA.ITS2_lg
Submitter Id
GACGATG
TagFWD
GTCGCCA
TagREV
FWD: GACGATG REV: GTCGCCA
adapters
ENVO:01000245
broad-scale environmental context
2020-10-07
collection date
Metabarcoding of nuclear ITS2 long fragment with multiplex-PCR from eDNA extracted from debris from nesting aids deployed on agricultural landscapes in Germany. Simultaneous amplification of mtCOI and ITS2 fragments.
description
ENVO:02000004
environmental medium
Germany
geographic location (country and/or sea)
51.68981629
DD
geographic location (latitude)
12.82736103
DD
geographic location (longitude)
51.68981629018775
lat
nest tube of cavity-nesting hymenoptera
local environmental context
12.827361028695893
lon
FWD: GACGATG REV: GTCGCCA
multiplex identifiers
PCR
nucleic acid amplification
Machery-Nagel NucleoSpin 96 Soil Kit
nucleic acid extraction
insect work metagenome
organism
FWD: GCTATGCGCGAGCtgcATGCGATACTTGGTGTGAAT REV: GCGAGCTGCGCTAtgcGACGCTTCTCCAGACTACAAT
pcr primers
PilotNA
project name
2020-10-07
removal_date
insect work metagenome
scientific_name
Greudnitz
site
GR
site_ID
10_113_R3S3
source material identifiers
ITS2
target gene
barcoding region
target subfragment
Greudnitz 10_113_R3S3 ITS2 metabarcoding from multiplexed PCR, Qiagen kit
title
10_113_R3S3
trap_ID