2025-08-01
ENA first public
ERC000011
ENA-CHECKLIST
Institute of Biology, Biotechnology and Environmental Protection, Faculty of Natural Sciences, University of Silesia in Katowice, Katowice, Poland
INSDC center name
public
INSDC status
ERS23771427
SRA accession
E-MTAB-14895:wt_dgt_2
Submitter Id
Growth stage 60-69, Zadoks scale
day
age
ArrayExpress
broker name
not collected
collection date
barley
common name
Sebastian
cultivar
Protocols: Tissue was collected from the middle part of the second leaf below the flag leaf in four biological replications. Each biological replication contained a fragment of leave from three independent plants. Seed were germinated on Petri dishes at 28°C in a growth chamber. After 5 days seedlings were transplanted to seedling pots and grown at 25°C. On the 20th day of growth plants were placed in 3L pots filled with a mixture of coarse sand with loam (1:3) and maintained under optimal water supply in greenhouse (22/16°C day/night, photoperiod 16/8h). 9 selected plants of each genotype were transferred to a PlantArray intelligent screening system (Plant-DiTech) on the 85th day of growth.  PlantArray lysimeter allows for strict monitoring of the water regimen and accurate drought application based on regular weight measurements and tracking of selected physiological parameters (Appiah et al. 2023). On the 99th day of growth (heading time, growth stage 50-59, Zadoks et al. 1974) 5 of the 9 plants of each genotype growing on a lysimeter were subjected to drought stress. Drought was applied for 15 days. After drought stress (flowering, growth stage 60-69, Zadoks et al. 1974) tissue collection for RNA-seq was performed. RNA was extracted using a mirVana RNA isolation kit (Thermo Fisher Scientific) according to the manufacturer’s instructions. True Seq libraries were constructed for each sample. PE 150.
description
Heading/Flowering
developmental stage
wild type genotype
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Hordeum vulgare
organism
Hordeum vulgare
organism
Second leave below the flag leaf
organism part
Hordeum vulgare
scientific_name
Drought
stimulus
wt_dgt_2
title