2025-08-01
ENA first public
ERC000011
ENA-CHECKLIST
Microbiology_UR
INSDC center name
public
INSDC status
ERS25350973
SRA accession
hvo_htq_nacl25_3
Submitter Id
2020
collection date
To investigate the effects of osmotic stress and c-di-AMP depletion on the transcriptome of Haloferax volcanii, we compared the transcriptional profiles of wild-type and c-di-AMP-reduced strains under both normal (2.5 M NaCl) and hypo-osmotic (1.8 M NaCl) conditions. Experiments were conducted using the H26 wild-type strain and the HTQ410 strain (c-di-AMP-reduced), in which the dacZ (HVO_1660) gene is placed under the control of a genetically engineered version of the tryptophan-inducible promoter (p.tnaA) of the tryptophanase gene tnaA (HVO_0789) (PMID: 30884174 ). Selected deletion strains were cultured overnight in YPC medium. Total RNA was extracted from H. volcanii strains H26 and HTQ410 cultivated to exponential phase in CAB medium using the RNeasy� Plus Mini Kit (Qiagen), following the manufacturer�s instructions.
description
Israel
geographic location (country and/or sea)
Haloferax volcanii DS2
organism
Haloferax volcanii DS2
scientific_name
HTQ410
strain
Haloferax volcanii HTQ410 normal salt - replicate 3
title