2025-08-01
ENA first public
ERC000025
ENA-CHECKLIST
Thuenen-Institute of Biodiversity
INSDC center name
public
INSDC status
NEB
Marker
GCTATGCGCGAGCtgcGGDACWGGWTGAACWGTWTAYCCHCC
PrimerSeq_FWD_1
GCTATGCGCGAGCtgcATGCGATACTTGGTGTGAAT
PrimerSeq_FWD_2
GCGAGCTGCGCTAtgcTAAACTTCAGGGTGACCAAAAAATCA
PrimerSeq_REV_1
GCGAGCTGCGCTAtgcGACGCTTCTCCAGACTACAAT
PrimerSeq_REV_2
ERS20884976
SRA accession
F-HEAD-23-R-TAIL-4
SampleID_ASV
109
SampleName
F-HEAD-23-R-TAIL-4.NEB.COI_lg
Submitter Id
TCAACCT
TagFWD
CTGCGAG
TagREV
FWD: TCAACCT REV: CTGCGAG
adapters
ENVO:01000245
broad-scale environmental context
2020-10-07
collection date
Metabarcoding of mtCOI long fragment with multiplex-PCR from eDNA extracted from debris from nesting aids deployed on agricultural landscapes in Germany. Simultaneous amplification of mtCOI and ITS2 fragments.
description
ENVO:02000004
environmental medium
Germany
geographic location (country and/or sea)
51.68774628
DD
geographic location (latitude)
12.80633203
DD
geographic location (longitude)
51.68774628069419
lat
nest tube of cavity-nesting hymenoptera
local environmental context
12.806332027602169
lon
FWD: TCAACCT REV: CTGCGAG
multiplex identifiers
PCR
nucleic acid amplification
Machery-Nagel NucleoSpin 96 Soil Kit
nucleic acid extraction
insect work metagenome
organism
FWD: GCTATGCGCGAGCtgcGGDACWGGWTGAACWGTWTAYCCHCC REV: GCGAGCTGCGCTAtgcTAAACTTCAGGGTGACCAAAAAATCA
pcr primers
PilotNA
project name
2020-10-07
removal_date
insect work metagenome
scientific_name
Greudnitz
site
GR
site_ID
10_109_R2S1
source material identifiers
mtCOI
target gene
long barcoding region
target subfragment
Greudnitz 10_109_R2S1 mtCOI metabarcoding from multiplexed PCR, NEB kit
title
10_109_R2S1
trap_ID