2025-08-01
ENA first public
ERC000025
ENA-CHECKLIST
Thuenen-Institute of Biodiversity
INSDC center name
public
INSDC status
NEB
Marker
GCTATGCGCGAGCtgcGGDACWGGWTGAACWGTWTAYCCHCC
PrimerSeq_FWD_1
GCTATGCGCGAGCtgcATGCGATACTTGGTGTGAAT
PrimerSeq_FWD_2
GCGAGCTGCGCTAtgcTAAACTTCAGGGTGACCAAAAAATCA
PrimerSeq_REV_1
GCGAGCTGCGCTAtgcGACGCTTCTCCAGACTACAAT
PrimerSeq_REV_2
ERS20884035
SRA accession
F-HEAD-23-R-TAIL-8
SampleID_ASV
020
SampleName
F-HEAD-23-R-TAIL-8.NEB.ITS2_lg
Submitter Id
TCAACCT
TagFWD
GTACATT
TagREV
FWD: TCAACCT REV: GTACATT
adapters
ENVO:01000245
broad-scale environmental context
2020-10-05
collection date
Metabarcoding of nuclear ITS2 long fragment with multiplex-PCR from eDNA extracted from debris from nesting aids deployed on agricultural landscapes in Germany. Simultaneous amplification of mtCOI and ITS2 fragments.
description
ENVO:02000004
environmental medium
Germany
geographic location (country and/or sea)
52.11614306
DD
geographic location (latitude)
11.96543900
DD
geographic location (longitude)
52.116143060418366
lat
nest tube of cavity-nesting hymenoptera
local environmental context
11.965439004770628
lon
FWD: TCAACCT REV: GTACATT
multiplex identifiers
PCR
nucleic acid amplification
Machery-Nagel NucleoSpin 96 Soil Kit
nucleic acid extraction
insect work metagenome
organism
FWD: GCTATGCGCGAGCtgcATGCGATACTTGGTGTGAAT REV: GCGAGCTGCGCTAtgcGACGCTTCTCCAGACTACAAT
pcr primers
PilotNA
project name
2020-10-05
removal_date
insect work metagenome
scientific_name
Dalchau
site
DA
site_ID
2_020_R2S1
source material identifiers
ITS2
target gene
barcoding region
target subfragment
Dalchau 2_020_R2S1 ITS2 metabarcoding from multiplexed PCR, NEB kit
title
2_020_R2S1
trap_ID