2025-08-01
ENA first public
ERC000025
ENA-CHECKLIST
Thuenen-Institute of Biodiversity
INSDC center name
public
INSDC status
QIA
Marker
GCTATGCGCGAGCtgcGGDACWGGWTGAACWGTWTAYCCHCC
PrimerSeq_FWD_1
GCTATGCGCGAGCtgcATGCGATACTTGGTGTGAAT
PrimerSeq_FWD_2
GCGAGCTGCGCTAtgcTAAACTTCAGGGTGACCAAAAAATCA
PrimerSeq_REV_1
GCGAGCTGCGCTAtgcGACGCTTCTCCAGACTACAAT
PrimerSeq_REV_2
ERS20884289
SRA accession
F-HEAD-1-R-TAIL-4
SampleID_ASV
044
SampleName
F-HEAD-1-R-TAIL-4.QIA.ITS2_lg
Submitter Id
CTCCCTG
TagFWD
CTGCGAG
TagREV
FWD: CTCCCTG REV: CTGCGAG
adapters
ENVO:01000245
broad-scale environmental context
2020-10-09
collection date
Metabarcoding of nuclear ITS2 long fragment with multiplex-PCR from eDNA extracted from debris from nesting aids deployed on agricultural landscapes in Germany. Simultaneous amplification of mtCOI and ITS2 fragments.
description
ENVO:02000004
environmental medium
Germany
geographic location (country and/or sea)
52.31515201
DD
geographic location (latitude)
11.16486100
DD
geographic location (longitude)
52.31515200746468
lat
nest tube of cavity-nesting hymenoptera
local environmental context
11.164861000463244
lon
FWD: CTCCCTG REV: CTGCGAG
multiplex identifiers
PCR
nucleic acid amplification
Machery-Nagel NucleoSpin 96 Soil Kit
nucleic acid extraction
insect work metagenome
organism
FWD: GCTATGCGCGAGCtgcATGCGATACTTGGTGTGAAT REV: GCGAGCTGCGCTAtgcGACGCTTCTCCAGACTACAAT
pcr primers
PilotNA
project name
2020-10-09
removal_date
insect work metagenome
scientific_name
Behnsdorf
site
BE
site_ID
4_044_R1S1
source material identifiers
ITS2
target gene
barcoding region
target subfragment
Behnsdorf 4_044_R1S1 ITS2 metabarcoding from multiplexed PCR, Qiagen kit
title
4_044_R1S1
trap_ID