2025-08-01
ENA first public
ERC000025
ENA-CHECKLIST
Thuenen-Institute of Biodiversity
INSDC center name
public
INSDC status
QIA
Marker
GCTATGCGCGAGCtgcGGDACWGGWTGAACWGTWTAYCCHCC
PrimerSeq_FWD_1
GCTATGCGCGAGCtgcATGCGATACTTGGTGTGAAT
PrimerSeq_FWD_2
GCGAGCTGCGCTAtgcTAAACTTCAGGGTGACCAAAAAATCA
PrimerSeq_REV_1
GCGAGCTGCGCTAtgcGACGCTTCTCCAGACTACAAT
PrimerSeq_REV_2
ERS20884785
SRA accession
F-HEAD-8-R-TAIL-4
SampleID_ASV
090
SampleName
F-HEAD-8-R-TAIL-4.QIA.COI_lg
Submitter Id
TTTGCTA
TagFWD
CTGCGAG
TagREV
FWD: TTTGCTA REV: CTGCGAG
adapters
ENVO:01000245
broad-scale environmental context
2020-10-08
collection date
Metabarcoding of mtCOI long fragment with multiplex-PCR from eDNA extracted from debris from nesting aids deployed on agricultural landscapes in Germany. Simultaneous amplification of mtCOI and ITS2 fragments.
description
ENVO:02000004
environmental medium
Germany
geographic location (country and/or sea)
51.40237504
DD
geographic location (latitude)
11.78100500
DD
geographic location (longitude)
51.402375041368984
lat
nest tube of cavity-nesting hymenoptera
local environmental context
11.78100500295243
lon
FWD: TTTGCTA REV: CTGCGAG
multiplex identifiers
PCR
nucleic acid amplification
Machery-Nagel NucleoSpin 96 Soil Kit
nucleic acid extraction
insect work metagenome
organism
FWD: GCTATGCGCGAGCtgcGGDACWGGWTGAACWGTWTAYCCHCC REV: GCGAGCTGCGCTAtgcTAAACTTCAGGGTGACCAAAAAATCA
pcr primers
PilotNA
project name
2020-10-08
removal_date
insect work metagenome
scientific_name
Steuden
site
ST
site_ID
8_090_R2S3
source material identifiers
mtCOI
target gene
long barcoding region
target subfragment
Steuden 8_090_R2S3 mtCOI metabarcoding from multiplexed PCR, Qiagen kit
title
8_090_R2S3
trap_ID