2025-08-01
ENA first public
ERC000025
ENA-CHECKLIST
Thuenen-Institute of Biodiversity
INSDC center name
public
INSDC status
ITS2.lg
Marker
GCTATGCGCGAGCtgcATGCGATACTTGGTGTGAAT
PrimerSeq_FWD_1
NA
PrimerSeq_FWD_2
GCGAGCTGCGCTAtgcGACGCTTCTCCAGACTACAAT
PrimerSeq_REV_1
NA
PrimerSeq_REV_2
ERS20883947
SRA accession
F-HEAD-17-R-TAIL-6
SampleID_ASV
013
SampleName
F-HEAD-17-R-TAIL-6.ITS2_lg
Submitter Id
TCAGTTG
TagFWD
TACGCGA
TagREV
FWD: TCAGTTG REV: TACGCGA
adapters
ENVO:01000245
broad-scale environmental context
2020-10-05
collection date
Metabarcoding of nuclear ITS2 long fragment from eDNA extracted from debris in nesting aids deployed on agricultural landscapes in Germany
description
ENVO:02000004
environmental medium
Germany
geographic location (country and/or sea)
52.13417806
DD
geographic location (latitude)
11.96854100
DD
geographic location (longitude)
52.13417806077779
lat
nest tube of cavity-nesting hymenoptera
local environmental context
11.968541004808744
lon
FWD: TCAGTTG REV: TACGCGA
multiplex identifiers
PCR
nucleic acid amplification
Machery-Nagel NucleoSpin 96 Soil Kit
nucleic acid extraction
insect work metagenome
organism
FWD: GCTATGCGCGAGCtgcATGCGATACTTGGTGTGAAT REV: GCGAGCTGCGCTAtgcGACGCTTCTCCAGACTACAAT
pcr primers
PilotNA
project name
2020-10-05
removal_date
insect work metagenome
scientific_name
Dalchau
site
DA
site_ID
2_013_R1S2
source material identifiers
ITS2
target gene
barcoding region
target subfragment
Dalchau 2_013_R1S2 ITS2 metabarcoding
title
2_013_R1S2
trap_ID