2025-08-01
ENA first public
ERC000025
ENA-CHECKLIST
Thuenen-Institute of Biodiversity
INSDC center name
public
INSDC status
QIA
Marker
GCTATGCGCGAGCtgcGGDACWGGWTGAACWGTWTAYCCHCC
PrimerSeq_FWD_1
GCTATGCGCGAGCtgcATGCGATACTTGGTGTGAAT
PrimerSeq_FWD_2
GCGAGCTGCGCTAtgcTAAACTTCAGGGTGACCAAAAAATCA
PrimerSeq_REV_1
GCGAGCTGCGCTAtgcGACGCTTCTCCAGACTACAAT
PrimerSeq_REV_2
ERS20884810
SRA accession
F-HEAD-7-R-TAIL-1
SampleID_ASV
092
SampleName
F-HEAD-7-R-TAIL-1.QIA.ITS2_lg
Submitter Id
CCAGATA
TagFWD
TCTACTG
TagREV
FWD: CCAGATA REV: TCTACTG
adapters
ENVO:01000245
broad-scale environmental context
2020-10-08
collection date
Metabarcoding of nuclear ITS2 long fragment with multiplex-PCR from eDNA extracted from debris from nesting aids deployed on agricultural landscapes in Germany. Simultaneous amplification of mtCOI and ITS2 fragments.
description
ENVO:02000004
environmental medium
Germany
geographic location (country and/or sea)
51.39416404
DD
geographic location (latitude)
11.77952800
DD
geographic location (longitude)
51.394164041241446
lat
nest tube of cavity-nesting hymenoptera
local environmental context
11.779528002940507
lon
FWD: CCAGATA REV: TCTACTG
multiplex identifiers
PCR
nucleic acid amplification
Machery-Nagel NucleoSpin 96 Soil Kit
nucleic acid extraction
insect work metagenome
organism
FWD: GCTATGCGCGAGCtgcATGCGATACTTGGTGTGAAT REV: GCGAGCTGCGCTAtgcGACGCTTCTCCAGACTACAAT
pcr primers
PilotNA
project name
2020-10-08
removal_date
insect work metagenome
scientific_name
Steuden
site
ST
site_ID
8_092_R1S1
source material identifiers
ITS2
target gene
barcoding region
target subfragment
Steuden 8_092_R1S1 ITS2 metabarcoding from multiplexed PCR, Qiagen kit
title
8_092_R1S1
trap_ID