2025-08-01
ENA first public
ERC000025
ENA-CHECKLIST
Thuenen-Institute of Biodiversity
INSDC center name
public
INSDC status
NEB
Marker
GCTATGCGCGAGCtgcGGDACWGGWTGAACWGTWTAYCCHCC
PrimerSeq_FWD_1
GCTATGCGCGAGCtgcATGCGATACTTGGTGTGAAT
PrimerSeq_FWD_2
GCGAGCTGCGCTAtgcTAAACTTCAGGGTGACCAAAAAATCA
PrimerSeq_REV_1
GCGAGCTGCGCTAtgcGACGCTTCTCCAGACTACAAT
PrimerSeq_REV_2
ERS20884551
SRA accession
F-HEAD-16-R-TAIL-4
SampleID_ASV
068
SampleName
F-HEAD-16-R-TAIL-4.NEB.COI_lg
Submitter Id
TGGTTAG
TagFWD
CTGCGAG
TagREV
FWD: TGGTTAG REV: CTGCGAG
adapters
ENVO:01000245
broad-scale environmental context
2020-10-06
collection date
Metabarcoding of mtCOI long fragment with multiplex-PCR from eDNA extracted from debris from nesting aids deployed on agricultural landscapes in Germany. Simultaneous amplification of mtCOI and ITS2 fragments.
description
ENVO:02000004
environmental medium
Germany
geographic location (country and/or sea)
51.87948179
DD
geographic location (latitude)
12.10712883
DD
geographic location (longitude)
51.87948179170424
lat
nest tube of cavity-nesting hymenoptera
local environmental context
12.107128829046287
lon
FWD: TGGTTAG REV: CTGCGAG
multiplex identifiers
PCR
nucleic acid amplification
Machery-Nagel NucleoSpin 96 Soil Kit
nucleic acid extraction
insect work metagenome
organism
FWD: GCTATGCGCGAGCtgcGGDACWGGWTGAACWGTWTAYCCHCC REV: GCGAGCTGCGCTAtgcTAAACTTCAGGGTGACCAAAAAATCA
pcr primers
PilotNA
project name
2020-10-06
removal_date
insect work metagenome
scientific_name
Rietzmeck
site
RI
site_ID
6_068_R4S3
source material identifiers
mtCOI
target gene
long barcoding region
target subfragment
Rietzmeck 6_068_R4S3 mtCOI metabarcoding from multiplexed PCR, NEB kit
title
6_068_R4S3
trap_ID