2025-08-04
ENA first public
ERC000011
ENA-CHECKLIST
SAMEA114236628
External Id
Department of Pathology, University of Cambridge
INSDC center name
2023-08-04T10:00:57Z
INSDC last update
public
INSDC status
ERS16222067
SRA accession
E-MTAB-13211:Sample 2
Submitter Id
ArrayExpress
broker name
immortalised bone marrow derived macrophage
cell_line
macrophage
cell_type
not collected
collection date
house mouse
common name
Protocols: Immortalised bone marrow derived macrophages were cultured overnight in DMEM supplemented with 10% fetal bovine serum. Culture supernatant was removed and replaced with either fresh media or conditioned media (i.e. the original culture supernatant). RNA was extracted from cultured cells by Trizol purification according to manufacturer's instructions Libraries were generated with the Lexogen QuantSeq 3' mRNA-Seq Library Prep Kit FWD according to manufacturer's instructions
description
adult
developmental stage
normal
disease
wild type genotype
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Mus musculus
organism
Mus musculus
organism
bone marrow
organism part
Mus musculus
scientific_name
Sample 2
title