2025-08-05
ENA first public
ERC000011
ENA-CHECKLIST
Berlin Institute for  Medical Systems Biology
INSDC center name
public
INSDC status
ERS20877436
SRA accession
E-MTAB-14354:iMN_FUSR244RR_1
Submitter Id
ArrayExpress
broker name
hiPSC
cell_line
induced motor neuron
cell_type
not collected
collection date
human
common name
Protocols: Pateint-derived iPSC with FUS(R244RR) and isogenic line with NGN2, ISL1, and LHX3 cassettes were differentiated into induced motor neurons (iMNs) using established protocols (Fernandopulle et al., 2018, doi: 10.1002/cpcb.51). Cells were grown on filter membrane inserts (pore size 1.0 μm PET membrane for 6-well plates Millicell PLRP06H48, Millipore) to isolate axonal and somatodendritic compartments. Whole iMNs and subcellular compartments were collected on day 21 of the differentiation protocol. RNA was extracted from using TRIfast (PeqLab 30-2010). For a detailed description of harvesting material from filter membranes see our prior work (Ludwik et al., 2019, doi: 10.1016/j.ymeth.2019.02.002).  100 ng of total RNA obtained from either total cells or separated compartments was used for library preparation with the Truseq stranded mRNA library prep kit (Illumina 20020595) according to the manufacturer’s recommendation.
description
motor neuron differential protocol
differentiation
ALS
disease
FUSR244RR
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
whole induced motor neuron
organism part
pluripotent stem cell
progenitor cell type
Homo sapiens
scientific_name
iMN_FUSR244RR_1
title