2025-08-06
ENA first public
ERC000011
ENA-CHECKLIST
Berlin Institute for  Medical Systems
INSDC center name
public
INSDC status
ERS20877827
SRA accession
E-MTAB-14359:iMN_day2_3
Submitter Id
ArrayExpress
broker name
hiPSC
cell_line
induced motor neuron
cell_type
not collected
collection date
human
common name
Protocols: hiPSCs with NGN2, ISL1, and LHX3 cassettes were differentiated into induced motor neurons (iMNs) using established protocols (Fernandopulle et al., 2018, doi: 10.1002/cpcb.51). RNA was collected in triplicates on days 1, 2, 4, 7, 14, and 21 of differentiation. RNA was extracted from using TRIfast (PeqLab 30-2010). For a detailed description of harvesting material from filter membranes see our prior work (Ludwik et al., 2019, doi: 10.1016/j.ymeth.2019.02.002).  100 ng of total RNA obtained from either total cells or separated compartments was used for library preparation with the Truseq stranded total library prep kit (Illumina 20020596) according to the manufacturer’s recommendation.
description
differentiation protocol
differentiation
normal
disease
wild type genotype
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
pluripotent stem cell
progenitor cell type
Homo sapiens
scientific_name
iMN_day2_3
title