2025-08-07
ENA first public
ERC000011
ENA-CHECKLIST
Hannover Medical School
INSDC center name
public
INSDC status
ERS20877778
SRA accession
E-MTAB-14356:FVV21
Submitter Id
ArrayExpress
broker name
endothelial colony forming cell
cell_type
not collected
collection date
human
common name
Protocols: We centrifuged umbilical cord blood and separated plasma for storage at -80°C. After density gradient centrifugation, the cord blood mononuclear cell fraction was plated at 5 x 107 cells/well on collagen coated 6-well plates ((BioCoat; Corning, USA) and incubated at 37 °C and 5 % CO2. Cells were grown with endothelial basal medium (EBM-2, Lonza, Basel, Switzerland) supplemented with 10% fetal bovine serum (FBS, Harvard Bioscience, Harvard Bioscience, Holliston, USA), 1% penicillin/streptomycin (P/S, Bio&Sell, Nürnberg, Germany) and recommended supplements. After 10 to 21 days, ECFC colonies appeared as adherent single layers of cobblestone-shaped cells. ECFC phenotype was confirmed by flow cytometry assessing CD31 (130-117-390; BD Biosciences San Jose, USA), CD45 (555483; BD Biosciences), CD133 (130-090-826; Miltenyi Biotec, Bergisch Gladbach, Germany) and the corresponding isotype controls. Total RNA was purified from ECFC lysates using the RNeasy Plus Mini Kit (Qiagen, Hilden, Germany). rRNA was depleted and PolyA enriched without globin depletion. A strand-specific library was utilized with the protocol NEB Ultra Directional RNA.
description
adult
developmental stage
Normal pregnancy
disease
not collected
geographic location (country and/or sea)
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
umbilical cord blood
organism part
Homo sapiens
scientific_name
FVV21
title