2025-08-08
ENA first public
ERC000011
ENA-CHECKLIST
Wellcome Sanger Institute and European Bioinformatics Institute (EMBL-EBI)
INSDC center name
public
INSDC status
ERS25424777
SRA accession
E-MTAB-15479:HD_F_GON13254253
Submitter Id
ArrayExpress
broker name
not collected
collection date
human
common name
Protocols: Single-nuclei suspensions were isolated from dissociated cells when performing scATAC-seq, following the manufacturers’ instructions, and from frozen tissue sections when performing multiomic snRNA-seq/scATAC-seq. For the latter, thick (300 µm) sections were cryosectioned, and kept in a tube on dry ice until subsequent processing. Nuclei were released via Dounce homogenisation as described in detail in the protocols.io.  For the scATAC-seq and multimodal snRNA-seq/scATAC-seq experiments, cells were loaded according to the manufacturer’s protocol for the Chromium Single Cell ATAC v2  and Chromium Next GEM Single Cell Multiome ATAC+Gene Expression (10X Genomics) to attain between 2,000 and 10,000 cells per well. Library preparation was carried out according to the manufacturer’s protocol.
description
9 PCW
developmental stage
not collected
geographic location (country and/or sea)
Hrv201
individual
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
full reproductive tract
organism part
Homo sapiens
scientific_name
female
sex
HD_F_GON13254253
title