2025-08-08
ENA first public
ERC000011
ENA-CHECKLIST
Wellcome Sanger Institute and European Bioinformatics Institute (EMBL-EBI)
INSDC center name
public
INSDC status
ERS25424769
SRA accession
E-MTAB-15479:HCA_F_GON11173192_and_HCA_F_GON11212447
Submitter Id
ArrayExpress
broker name
not collected
collection date
human
common name
Protocols: Single-nuclei suspensions were isolated from dissociated cells when performing scATAC-seq, following the manufacturers’ instructions, and from frozen tissue sections when performing multiomic snRNA-seq/scATAC-seq. For the latter, thick (300 µm) sections were cryosectioned, and kept in a tube on dry ice until subsequent processing. Nuclei were released via Dounce homogenisation as described in detail in the protocols.io.  For the scATAC-seq and multimodal snRNA-seq/scATAC-seq experiments, cells were loaded according to the manufacturer’s protocol for the Chromium Single Cell ATAC v2  and Chromium Next GEM Single Cell Multiome ATAC+Gene Expression (10X Genomics) to attain between 2,000 and 10,000 cells per well. Library preparation was carried out according to the manufacturer’s protocol.
description
12 PCW
developmental stage
not collected
geographic location (country and/or sea)
Hrv103
individual
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
fallopian tube, uterus, cervix
organism part
Homo sapiens
scientific_name
female
sex
HCA_F_GON11173192_and_HCA_F_GON11212447
title