2025-08-08
ENA first public
ERC000011
ENA-CHECKLIST
IFOM ETS - The AIRC Institute of Molecular Oncology
INSDC center name
public
INSDC status
ERS20949542
SRA accession
E-MTAB-14421:S3_CTRL
Submitter Id
7
week
age
ArrayExpress
broker name
not collected
collection date
house mouse
common name
Protocols: When tumors became palpable, mice were randomly assigned to ad libitum diet (comtrol arm) or cyclic fasting (experimental arm). At the end of the experiments, mice were euthanized by using CO2, and tumor masses collected. RNA was extracted from tumor masses collected after the second cycle of fasting, or at the corresponding timepoint in the control group. RNA was extracted from snap-frozen tumor masses using the RNeasy Mini Kit (QIAGEN). For each sample, 500 ng of total RNA were used to generate a library of fragments using Illumina Stranded mRNA prep ligation kit. Oligo(dT) magnetic beads purified and captured mRNA molecules containing poly(A) tails. Then, purified mRNA was fragmented and copied into first strand complimentary DNA (cDNA) using reverse transcriptase and random primers. In a second strand cDNA synthesis step, dUTP replaced dTTP to achieve strand specificity. The final steps added adenine and thymine bases to fragment ends and ligated adapters. The resulting products were purified and selectively amplified with 12 cycles of PCR to generate an indexed library of fragments.
description
ad-libitum
diet
not collected
geographic location (country and/or sea)
not applicable
isolate
Mus musculus
organism
Mus musculus
organism
breast
organism part
tumor
sampling site
Mus musculus
scientific_name
BALB/c
strain
S3_CTRL
title