2025-08-08
ENA first public
ERC000011
ENA-CHECKLIST
Nuclear Dynamics Unit UMR3664 CNRS/IC Institut Curie - Centre de Recherche
INSDC center name
public
INSDC status
ERS20948847
SRA accession
E-MTAB-14419:H31SNAP_YFP_0h_input_r1
Submitter Id
ArrayExpress
broker name
HeLa
cell_line
epithelial cell
cell_type
not collected
collection date
human
common name
Protocols: For H3.1/H3.3-SNAP ChIP-seq, we collected either G1/S-arrested cells by a double thymidine block (t0) or asynchronous cells (asynch). For EdU-seq, used to map sites of ongoing DNA synthesis, we released G1/S synchronized cells in S-phase as described in (Gatto et al., 2022). Ninety minutes after release of cells from the G1/S block, we performed a 30min pulse labelling by adding EdU (25μM) to the medium. For all experiments, we collected 4 million cells/condition. We used HeLa cells stably expressing H3.1-SNAP-HA or H3.3-SNAP-HA which were knocked out for HIRA (KO, CRISPR/Cas9 HIRA KO) and transfected them with plasmids expressing with YFP (control) or HIRA-YFP 48 prior to collection as in (Ray-Gallet et al., 2018). Cell lines were grown and synchronized at the G1/S transition using a double thymidine block as described in (Forest et al., 2024; Gatto et al., 2022). All cell lines were tested negative for mycoplasma. We performed SNAP capture-seq of G1/S-synchronised cells by double thymidine block as described in (Forest et al., 2024; Gatto et al., 2022).  We prepared sequencing libraries at the Next Generation Sequencing (NGS) platform from Institut Curie with the Illumina TruSeq ChIP kit.
description
adult
developmental stage
cervical adenocarcinoma
disease
H3.1-SNAP-HA HIRA CRISPR/Cas9 KO + YFP
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
uterine cervix
organism part
1
replicate
Homo sapiens
scientific_name
H31SNAP_YFP_0h_input_r1
title