2025-08-08
ENA first public
ERC000011
ENA-CHECKLIST
Nuclear Dynamics Unit UMR3664 CNRS/IC Institut Curie - Centre de Recherche
INSDC center name
public
INSDC status
ERS20948791
SRA accession
E-MTAB-14415:WT_H31SNAP_r2
Submitter Id
ArrayExpress
broker name
HeLa
cell_line
epithelial cell
cell_type
not collected
collection date
human
common name
Protocols: Cells were collected by trypsinization (50000 cells per condition). To ensure cells remained in G1/S block, trypsin and PBS used for washes were supplemented with thymidine to 2mM final. We used HeLa cells stably expressing H3.3-SNAP-HA or H3.1-SNAP-HA, and derived HIRA knockout cell lines (HIRA CRISPR/Cas9 KO) or HIRA wild-type control cells (GFP CRISPR/Cas9 KO) as described in Ray-Gallet et al. 2019. We cultured cells in DMEM complete medium with D-Glucose, L-Glutamine and Pyruvate, supplemented with 10% fetal calf serum, 100 U/mL Penicillin and 100 µg/mL Streptomycin. Cells were synchronized in G1/S via double thymidine block: subsequent exposure to 2 mM thymidine (16-18h), 24 µM 2-Deoxycytidine (6h) and 2 mM thymidine (18h). Tagmentation was performed following the ATAC-seq kit protocol (Active Motif, 53150) Libraries were constructed following the ATAC-seq kit protocol (Active Motif, 53150).
description
adult
developmental stage
cervical adenocarcinoma
disease
H3.1-SNAP-HA GFP CRISPR/Cas9 KO
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
uterine cervix
organism part
2
replicate
Homo sapiens
scientific_name
WT_H31SNAP_r2
title