2025-08-09
ENA first public
ERC000011
ENA-CHECKLIST
Charles University Faculty of Science Department of Cell Biology
INSDC center name
public
INSDC status
ERS25426000
SRA accession
E-MTAB-15468:Sample 1
Submitter Id
14 (6 post bone marrow transplantation)
week
age
ArrayExpress
broker name
not collected
collection date
house mouse
common name
Protocols: The Defa6-iCre R26-TdTomato mouse model, which exhibits restricted expression of the TdTomato fluorescent protein in medullary thymic epithelial cells, was used as the recipient for competitive bone marrow chimeras composed of Claudin-1–sufficient and Claudin-1–deficient donor cells. After six weeks, thymi were dissected and type 1 dendritic cells arising from Claudin-1–sufficient and Claudin-1–deficient bone marrow were isolated by flow cytometric sorting from one individual animal. Cells were directly sorted into lysis buffer. Complete mRNA was isolated based on polyA selection using Dynabeads mRNA direct purification kit.  Sequencing libraries were prepared using the MARS-seq protocol, as described previously (Jaitin, D. A. et al. Massively parallel single-cell RNA-seq for marker-free decomposition of tissues into cell types. Science 343, 776–779 (2014)).
description
adult
developmental stage
wild type genotype
genotype
not collected
geographic location (country and/or sea)
1
individual
not applicable
isolate
Mus musculus
organism
Mus musculus
organism
thymus
organism part
Claudin 1
phonotype
Mus musculus
scientific_name
female
sex
C57BL/6J
strain
Sample 1
title