2025-08-09
ENA first public
ERC000011
ENA-CHECKLIST
1 Institute of Human Biology (IHB), Roche Pharma Research and Early Development, Roche Innovation Center Basel, Basel, Switzerland 2 Institute of Molecular and Clinical Ophthalmology Basel, Basel, Switzerland
INSDC center name
public
INSDC status
ERS24422699
SRA accession
E-MTAB-15112:GEX_Fetal_ProxSI_organoids_day5
Submitter Id
snRNA-seq
assay type
ArrayExpress
broker name
Developing intestine epithelial stem cells
cell line
Intestinal epithelial cell
cell type
not collected
collection date
human
common name
Protocols: Developing intestinal tissues and organoids samples were enzymatically and mechanically dissociated into single cells using the Neural Tissue Dissociation Kit (Miltenyi Biotec, 130-092-628). For scATAC and multiome, nuclei were isolated with a custom lysis buffer for developing human tissue, while 10x Genomics protocols were followed for all other samples. Specific human and chimpanzee organoid single cell suspensions were pooled at equal amount.  For all samples, nucleic acids were extracted and processed following the recommendations and standard protocols of 10x Genomics for the corresponding data type. For all samples, scRNA-seq, scATAC-seq and multiome libraries were generated on a 10x Chromium device following the recommendations and standard protocols of 10x Genomics for the corresponding data type.
description
Late embryonic stage
developmental stage
normal
disease
wild type genotype
genotype
not collected
geographic location (country and/or sea)
Wnt3a, Noggin, R-spondin-3, IGF1, FGF2, A-83-01, Gastrin
growth condition
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
intestine
organism part
Homo sapiens
scientific_name
In vitro enteroid culture
system
GEX_Fetal_ProxSI_organoids_day5
title