2025-08-09
ENA first public
ERC000011
ENA-CHECKLIST
1 Institute of Human Biology (IHB), Roche Pharma Research and Early Development, Roche Innovation Center Basel, Basel, Switzerland 2 Institute of Molecular and Clinical Ophthalmology Basel, Basel, Switzerland
INSDC center name
public
INSDC status
ERS24422713
SRA accession
E-MTAB-15112:C2-CIO-D47_H9-HIO-D33_ATAC_pooled
Submitter Id
scATAC-seq
assay type
ArrayExpress
broker name
C2 / H9
cell line
Intestinal multilineage cell
cell type
not collected
collection date
Protocols: Developing intestinal tissues and organoids samples were enzymatically and mechanically dissociated into single cells using the Neural Tissue Dissociation Kit (Miltenyi Biotec, 130-092-628). For scATAC and multiome, nuclei were isolated with a custom lysis buffer for developing human tissue, while 10x Genomics protocols were followed for all other samples. Specific human and chimpanzee organoid single cell suspensions were pooled at equal amount.  For all samples, nucleic acids were extracted and processed following the recommendations and standard protocols of 10x Genomics for the corresponding data type. For all samples, scRNA-seq, scATAC-seq and multiome libraries were generated on a 10x Chromium device following the recommendations and standard protocols of 10x Genomics for the corresponding data type.
description
Early embryonic stage
developmental stage
normal
disease
wild type genotype
genotype
not collected
geographic location (country and/or sea)
EGF, Noggin, R-spondin-1
growth condition
not applicable
isolate
mixed sample
organism
mixed sample
organism
intestine
organism part
mixed sample
scientific_name
In vitro multilineage organoid
system
C2-CIO-D47_H9-HIO-D33_ATAC_pooled
title