2025-08-09
ENA first public
ERC000011
ENA-CHECKLIST
Institute of Human Biology (IHB)
INSDC center name
public
INSDC status
ERS23862128
SRA accession
E-MTAB-14972:Batch 2
Submitter Id
ArrayExpress
broker name
not collected
collection date
Protocols: DNA sequences of candidate cis-regulatory elements (CREs) were synthesized using a commercial service (GenScript) and cloned individually into the STARR-seq vector via Gibson assembly. Endotoxin-free plasmids were purified with ZymoPURE II Plasmid Maxiprep kit (Zymo) and DNA precipitation with 3M Na-Acetate pH 5.2 was performed if necessary to obtain desired concentrations. The sequencing library was generated with the NEBNext Ultra II FS DNA Library Prep Kit (New England Biolabs)
description
wild type genotype
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
synthetic construct
organism
synthetic construct
organism
synthetic
organism part
2
replicate
synthetic construct
scientific_name
Batch 2
title