2025-08-13
ENA first public
ERC000011
ENA-CHECKLIST
MPUSP
INSDC center name
public
INSDC status
ERS25446786
SRA accession
3PLibrary-t5-040-_S40
Submitter Id
Max Planck Unit for the Science of Pathogens
collected_by
2023-09-13
collection date
LB medium
culture_medium
Overnight cultures of  E.Coli BL21(DE3) carrying pCas4-1-2 and pCRISPR plasmids were grown in LB medium containing 1% glucose and selective antibiotics (50 �g/ml streptomycin (Sm) for pCas and 30 �g/ml chloramphenicol (Cm) for pCRISPR)at 37�C. The cultures were diluted 1:100 in LB medium containing Cm and Sm grown at 37�C until OD600 of 0.5. The cultures were pelleted and washed with fresh LB medium containing Cm and 0.5 mM IPTG, growth continued with agitation at 37�C for 20 h. Cells were collected and normalised to an OD600 nm of 1.0 in 200 �L, pelleted and resuspended in 100 �L ddH2O before storage at -20�C for further PCR reaction.
description
wild type
genotype
Germany
geographic location (country and/or sea)
Escherichia coli BL21(DE3)
organism
Escherichia coli BL21(DE3)
scientific_name
NA
serotype (required for a seropositive sample)
BL21
strain
BL21(DE3)
sub_strain
E.Coli BL21(DE3), pCas4-1-2, 37�C, Cm, replicate 1
title