2025-08-13
ENA first public
ERC000011
ENA-CHECKLIST
Laboratory of Molecular Medicine and Genomics, Department of Medicine, Surgery and Dentistry ‘Scuola Medica Salernitana’, University of Salerno, Baronissi (SA), Italy
INSDC center name
public
INSDC status
ERS25271981
SRA accession
E-MTAB-15370:CTRL_rep4
Submitter Id
ArrayExpress
broker name
not collected
collection date
human
common name
Protocols: Total RNA was isolated from adipose tissue samples obtained from 8 patients (4 with lipedema and 4 healthy individuals). Tissues were snap-frozen in liquid nitrogen immediately after collection and stored at −80 °C until further processing. Prior to RNA extraction, samples (~150 mg each) were cryopulverized using the CP02 cryoPREP® Automated Dry Pulverizer (Covaris).   RNA was extracted using TRIzol™ Reagent (Invitrogen, #15596026) according to the manufacturer's protocol. Briefly, 1 ml of TRIzol was added to ~150 mg of pulverized adipose tissue, followed by homogenization and phase separation. RNA was quantified with Qubit 2.0 fluorimeter using Qubit RNA HS assay kit (Termo Fisher Scientifc, USA), and the assessment of nucleic acids integrity (RNA Integrity Number) was performed with Agilent 4150 TapeStation System (Agilent Technologies, USA).  Indexed libraries were prepared starting from 200 ng total RNA according to TruSeq Stranded Total RNA Library Prep Gold (Cat. 20020599, Illumina, San Diego, California, USA)
description
normal
disease
not collected
geographic location (country and/or sea)
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
leg
organism part
adipose tissue
sampling site
Homo sapiens
scientific_name
female
sex
CTRL_rep4
title