2025-08-13
ENA first public
ERC000011
ENA-CHECKLIST
MPUSP
INSDC center name
public
INSDC status
ERS25446845
SRA accession
5P-L28635-3P-library-X0-D03_S39
Submitter Id
Max Planck Unit for the Science of Pathogens
collected_by
2023-03-31
collection date
LB medium
culture_medium
Overnight cultures of  E.Coli BL21(AI) carrying pCas12-1-2 and pCRISPR plasmids were grown in LB medium containing selective antibiotics (50 �g/ml streptomycin (Sm) for pCas and 30 �g/ml chloramphenicol (Cm) for pCRISPR)at 37�C. The cultures were diluted 1:100 in LB medium containing Cm and Sm grown at 37�C until OD600 of 0.5. The cultures were pelleted and washed with fresh LB medium containing Cm, 2g/L arabinose and 0.1 mM IPTG, growth continued with agitation at 30�C for 20 h. Cells were collected and normalised to an OD600 nm of 1.0 in 200 �L, pelleted and resuspended in 100 �L ddH2O before storage at -20�C for further PCR reaction.
description
wild type
genotype
Germany
geographic location (country and/or sea)
Escherichia coli BL21
organism
Escherichia coli BL21
scientific_name
NA
serotype (required for a seropositive sample)
BL21
strain
BL21(AI)
sub_strain
E.Coli BL21(AI), pCas12-1-2, 30�C, Cm, low IPTG, replicate 3
title