2025-08-15
ENA first public
ERC000011
ENA-CHECKLIST
ACTREC - Tata Memorial Centre
INSDC center name
public
INSDC status
ERS20224064
SRA accession
E-MTAB-14162:U87 RS rep2
Submitter Id
ArrayExpress
broker name
U87
cell_line
GBM
cell_type
not collected
collection date
human
common name
Protocols: GBM cells (P) were grown to 70-80% confluency and subjected to their respective lethal doses of radiation U87MG (10Gy) and PS2 (14Gy). After an initial death phase, a transiently non-proliferative residual population (RS) was conformed microscopically and by monitoring cell proliferation by trypan blue method. The residual cells which resumed proliferation were designated as end of residual (ERS). Cell population after complete recovery was designated as relapse (R).  RNA Isoplus (Takara) was used for isolation and enrichment of total RNA from parent, residual, end of residual and relapse GBM samples. TruSeq RNA library protocol (Illumina) was used for generation of libraries. Post QC verification, 4 μg of intact total RNA was used for mRNA purification through oligodT beads, and library preparation was carried out according to manufacturer’s guidelines using the TruSeq RNA Sample Preparation Kit (Illumina).
description
adult
developmental stage
brain glioblastoma
disease
diploid
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
brain
organism part
U87 non-proliferative residual population (RS)
phenotype
Homo sapiens
scientific_name
U87 RS rep2
title