2025-08-15
ENA first public
ERC000011
ENA-CHECKLIST
INGM, National Institute of Molecular Genetics, Milan, Italy
INSDC center name
public
INSDC status
ERS23661901
SRA accession
E-MTAB-14864:Sample 1
Submitter Id
ArrayExpress
broker name
MEF cell line
cell_line
not collected
collection date
house mouse
common name
Protocols: Immortalized MEF cells were washed once with ice-cold PBS 10ug/ml cycloheximide. digestion without KCl treatment Cells were lysed in 20mM Tris-HCl, 50mM NaCl, 10mM MgCl2, 1mM DTT, 100ug/ml cycloheximide, 1% NP-40, 0.2mg/ml heparin. Ribosome Protected Fragments (RPF) were obtained following Ingolia 2017 (10.1016/j.ymeth.2017.05.028).  Immagina Biotechnology LACEseq #LS-001_12
description
wild type genotype
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Mus musculus
organism
Mus musculus
organism
Embryonic fibroblasts
organism part
Mus musculus
scientific_name
Sample 1
title
None
treatment