2025-08-16
ENA first public
ERC000011
ENA-CHECKLIST
SAMEA114342001
External Id
Istituto Nazionale di Genetica Molecolare (INGM)
INSDC center name
2023-09-08T12:37:59Z
INSDC last update
public
INSDC status
ERS16331023
SRA accession
E-MTAB-13335:Sample 3
Submitter Id
ArrayExpress
broker name
mouse embryonic fibroblast
cell_line
fibroblast
cell_type
not collected
collection date
house mouse
common name
Protocols: Immortalized MEF cells were pre-treated with 2ug/ml harringtonine for 2 minutes followed by 100ug/ml cyclohexamide for other 2 minutes. Finally, the cells were washed once with ice-cold PBS 10ug/ml cyclohexamide. Cells were lysed in 20mM Tris-HCl, 50mM NaCl, 10mM MgCl2, 1mM DTT, 100ug/ml cyclohexamide, 1% NP-40, 0.2mg/ml heparin. Ribosome Protected Fragments (RPF) were obtained following Ingolia 2017 (10.1016/j.ymeth.2017.05.028). Immagina Biotechnology LACEseq #LS-001_12
description
embryo stage
developmental stage
Eif6 S235A
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Mus musculus
organism
Mus musculus
organism
mouse embryonic fibroblast cell
organism part
Mus musculus
scientific_name
Sample 3
title