2025-08-19
ENA first public
ERC000011
ENA-CHECKLIST
Brain Tumor Biology group, Division of Translational Cancer Research, Lund University
INSDC center name
public
INSDC status
ERS24839032
SRA accession
E-MTAB-15215:C3
Submitter Id
ArrayExpress
broker name
Human primary astrocytes (3H Biomedical - SC1800)
cell line
astrocyte
cell type
not collected
collection date
human
common name
Protocols: The cells used for the experiment are human primary astrocytes (3H Biomedical - SC1800). The cells received either DMSO or Flunarizine treatment at 10uM. 2 hours later, cells have been irradiated or not with 10Gy. 24 hours after irradiation the cells have been lysed and the RNA content extracted for bulk RNAseq analysis. 4 replicates have been made for each condition. Primary human astrocytes (3H Biomedical - SC1800-5) were cultured in astrocyte medium (3H Biomedical, SC1801) supplemented with 2% FBS, 1% PenStrep and astrocyte growth serum (provided by 3H Biomedical). one day after seeding, cells were treated with either DMSO or Flunarizine 10uM (MedChemExpress HY-B0358A) and 2 hours later irradiation was performed or not using a Cix1 x-ray irradiator (Xstrahl) at 10Gy. Cells were lysed 24 hours after irradiation The RNA content of the cells have been extracted RNeasy mini kit (Qiagen) together with the Qiashredder Kit (Qiagen) according to manufacturer’s protocols Protocol: Illumina Stranded mRNA Prep, Ligation Reference Guide (Document # 1000000124518 v03). Modification of “Enrich DNA Fragment” step: 12 PCR cycles. Reagent/kit: Illumina® Stranded mRNA Prep, Ligation (20091659, Illumina) Indexing: Illumina® RNA UD Indexes Set C, Ligation (96 Indexes, 20091655, Illumina) Equipment: Automatisation of Clean up steps: King Fisher FLEX (18-5400620, ThermoScientific), incubations and PCR: Eppendorf Mastercycler X50s (6311000010, Eppendorf)
description
fetal
developmental stage
Rep3_01
fastq_id
not collected
geographic location (country and/or sea)
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
cerebral cortex
organism part
3
replicate
C3
sample_id
Homo sapiens
scientific_name
C3
title