2025-08-22
ENA first public
ERC000011
ENA-CHECKLIST
EMBL
INSDC center name
public
INSDC status
ERS25351011
SRA accession
ena-upload-cli
SUBMISSION_TOOL
0.9.0
SUBMISSION_TOOL_VERSION
b43e7cae-c5ef-4de6-bdb0-ee52805bf754
Submitter Id
4-20 hrs
age
not provided
collection date
fruit fly
common name
Wildtype Drosophila melanogaster flies were grown in population cages at 25°C. MiMIC fly lines with boundary insertions were grown in large-sized cages at 25°C. 0-16 hour embryo collections were aged at 25°C up to the desired time point (4-20 hours). Two independent collections were performed for each timepoint. The collected embryos were dechorionated using 50% bleach and formaldehyde fixed in 10 ml cross-linking solution (50 mM Hepes, 1 mM EDTA, 0.5 mM EGTA, 100 mM NaCl, 1.8 % formaldehyde, pH 8.0) and 30 ml n-heptane on a shaker table at RT for 15 minutes. The reaction was terminated with 125 mM glycine, 0.1% Triton X-100 in PBS. After washing out the fix, the embryos were blotted dry and frozen in liquid nitrogen.
description
insertion
genetic modification
wild type
genotype
not applicable
geographic location (country and/or sea)
egg laying
initial time point
Drosophila melanogaster
organism
Drosophila melanogaster
scientific_name
mixed sex
sex
BDSC 6598
strain
WT
title