2025-08-22
ENA first public
ERC000011
ENA-CHECKLIST
Institute of Molecular Genetics of the Czech Academy of Sciences
INSDC center name
public
INSDC status
ERS22563481
SRA accession
E-MTAB-14685:PLT_MUT1
Submitter Id
12
week
age
ArrayExpress
broker name
platelet
cell_type
not collected
collection date
house mouse
common name
Protocols: For platelets (PLT) isolation, whole blood was collected to acid-citrate-dextrose tubes supplemented with 3 uM prostaglandin E1 (PGE), and PLT-rich plasma was collected by centrifugation. Pelleted PLTs were subjected to red cell lysis and resuspended in the presence of anti-CD45 beads (Milteney Biotec) to remove residual leukocytes. Total RNA was isolated using RNeasy Micro Kit (Qiagen) as described in the manufacturer’s protocol. Libraries were prepared with a SMARTer® Stranded Total RNA-Seq – Pico Input Mammalian library preparation kit v2 (Takara).
description
adult
developmental stage
homozygous Jak2 R1063H
genotype
not collected
geographic location (country and/or sea)
A
individual
not applicable
isolate
Mus musculus
organism
Mus musculus
organism
blood
organism part
Mus musculus
scientific_name
male
sex
C57BL/6
strain
PLT_MUT1
title