2025-08-24
ENA first public
ERC000011
ENA-CHECKLIST
National Institute of Immunology, New Delhi
INSDC center name
public
INSDC status
ERS22447989
SRA accession
E-MTAB-14629:Sample 8
Submitter Id
ArrayExpress
broker name
HCT116
cell_line
colorectal cancer cell
cell_type
not collected
collection date
human
common name
Protocols: Cells were harvested from 6 well-plate, followed by trypsinization, crosslinking and fixaton. Cells were grown under asynchronous condition in McCoy media HCT116 p21-/- cell were grown in 6 well plate, without any treatment.  HCT116 p53-/- p53 TAD mutant grown in 6 well in presence of Doxycycline. Cells were lysed using a nuclear lysis buffer. Followed by sonication of genomic DNA. 1ng of DNA used for library preparation. The Re-ChIP DNA libraries were constructed using the Kapa Hyperprep Kit with sample purification beads according to the manufacturer’s instructions.
description
colon carcinoma
disease
HCT116 p53-/- p53 TAD mutant
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
Homo sapiens
scientific_name
Sample 8
title