2025-08-26
ENA first public
ERC000013
ENA-CHECKLIST
Max Rubner-Institute
INSDC center name
public
INSDC status
ERS25491647
SRA accession
ena-SAMPLE-TAB-14-08-2025-09:35:37:519-75869
Submitter Id
fwd:ACACTCTTTCCCTACACGACGCTCTTCCGATCTNNNNNNCA::rev:CAAGCAGAAGACGGCATACGAGATNNNNNNGTGACTGGAGTTCAGACGTGTGCTCTTCCGATCT
adapters
ENVO_01001033:digestive tract environment
broad-scale environmental context
2016-06-14
collection date
mouse 98, week 10
description
BR
diet group
ENVO_00002003:fecal material
environmental medium
Germany
geographic location (country and/or sea)
49.0139822
DD
geographic location (latitude)
8.4240071
DD
geographic location (longitude)
18.000
weeks
host age
fma64183:Feces
host body product
Mouse
host common name
per kg: 200 g casein, 395 g corn starch, 50 g sucrose, 190 g lard, 10 g vitamin mix, 50 g mineral mix, 3 g L-cysteine, 3 g choline chloride, 100 g wheat bran
host diet
single housed in individually ventilated cages
host growth conditions
Mus musculus
host scientific name
male
host sex
98
host subject id
C57BL/6
host subspecific genetic lineage
10090
host taxid
ENVO_01001029:fecal environment
local environmental context
R1:ACCAAT_R2:CTGATC
multiplex identifiers
doi:10.3390/nu11102348
nucleic acid amplification
doi:10.3390/nu11102348
nucleic acid extraction
mouse gut metagenome
organism
3 PCR steps: enrichment PCR - 10 cycles, custom barcode integration - 15 cycles, illumina index integration - 20 cycles, see doi:10.3390/nu11102348 for detailed protocol
pcr conditions
fwd:AGAGTTTGATCMTGGCTCAG::rev:TGCTGCCTCCCGTAGGAGT
pcr primers
Whole Grain Mouse Gut Microbiome
project name
mouse gut metagenome
scientific_name
16S rRNA
target gene
V1V2
target subfragment
98_10
title
10
week-after-start