E-MTAB-14400:Trypomastigote 2-1
2025-08-27
ENA first public
ERC000011
ENA-CHECKLIST
University of Glasgow University of Edinburgh
INSDC center name
public
INSDC status
ERS20928046
SRA accession
E-MTAB-14400:Trypomastigote 2-1
Submitter Id
ArrayExpress
broker name
Trypomastigote
cell_type
not collected
collection date
Protocols: Amastigote derived trypomastigotes (ADT) were harvested from the supernatant of infected Vero cells at 7 dpi. EP, SE/MT and ADT were put in falcon tubes and left undisturbed for 1 h (at 28 °C for EP and SE/MT, and at 37 °C 5\% CO2 for ADT). To avoid collecting dead parasites and co-purified Vero cells, the supernatant, containing viable and motile cells, was collected. Parasites were pelleted and washed once in ice-cold phosphate-buffered saline (PBS) by centrifugation at 1,350 x g for 5 min at 4 °C. Total RNA was extracted using RNeasy Mini Kit (Qiagen, Hilder, Germany) following the manufacturer's protocol, with an on-column DNase digestion step with the RNAse-Free DNase Set (Qiagen).  Two biological replicates for each life cycle stage were prepared and sent to Novogene Co., Ltd (Cambridge, UK) for rRNA removal, library construction and sequencing. RNA quantity and integrity were measured with an Agilent 2100 bioanalyzer using the RNA Nano 6000 Assay Kit (Agilent Technologies, Santa Clara, CA, USA) and by agarose gel electrophoresis. A total of 1 µg total RNA per sample was used as input material for the lncRNA library preparation. Ribosomal RNA was depleted from total RNA and strand-specific libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (New England BioLabs, Ipswich, MA, USA) following manufacturer’s recommendations. Index codes were added to attribute sequences to each sample.
description
not collected
geographic location (country and/or sea)
not applicable
isolate
Trypanosoma cruzi
organism
Trypanosoma cruzi
organism
Trypanosoma cruzi
scientific_name
Trypomastigote 2-1
title