E-MTAB-13347:DMSO
2025-08-27
ENA first public
ERC000011
ENA-CHECKLIST
SAMEA114394053
External Id
Department of Biological Sciences, The University of Texas at Dallas
INSDC center name
2023-09-18T13:54:54Z
INSDC last update
public
INSDC status
ERS16383116
SRA accession
E-MTAB-13347:DMSO
Submitter Id
ArrayExpress
broker name
GM12878
cell_line
B lymphoblastoid
cell_type
not collected
collection date
human
common name
Protocols: When cells are 80% confluent, aphidicolin or DMSO at 1uM concentration was added to the media and incubated for 12 hours at 37C. Sendai Virus or media was added to the media and incubated for additional 12 hours at 37C. Cells were collected at the end of 24h incubation. GM12878 cells were cultured with RPMI media supplemented with 10% FBS 37C with 5% CO2. When cells are 80% confluent, aphidicolin or DMSO at 1uM concentration was added to the media and incubated for 12 hours at 37C. Sendai Virus or media was added to the media and incubated for 12 hours at 37C. RNA extraction from each cell was performed according to 10x Chromium Single Cell 3' protocol. Library construction was performed according to 10x Chromium Single Cell 3' protocol.
description
normal
disease
wild type genotype
genotype
not collected
geographic location (country and/or sea)
Sendai virus
infect
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
blood
organism part
Homo sapiens
scientific_name
DMSO
title