E-MTAB-13340:JU_AU_3
2025-08-27
ENA first public
ERC000011
ENA-CHECKLIST
SAMEA114349401
External Id
Department of Biological Sciences, The University of Texas at Dallas
INSDC center name
2023-09-11T22:26:32Z
INSDC last update
public
INSDC status
ERS16338445
SRA accession
E-MTAB-13340:JU_AU_3
Submitter Id
ArrayExpress
broker name
Jurkat E6.1
cell_line
T lymphoblast
cell_type
not collected
collection date
human
common name
Protocols: At the end of 24 hours, cells were washed with cold 1x PBS twice. Cells were collected in RLT buffer (Qiagen) supplemented with dTT as suggested by RNeasy mini RNA extraction protocol. GM12878 and JURKAT cells were cultured with RPMI media supplemented with 10% FBS 37C with 5% CO2. When cells are 80% confluent, 1 uM of aphidicolin was added to the media and incubated for 12 hours at 37C. Sendai Virus or media was added to the media and incubated for and additional of 12 hours at 37C. RNA was isolated using RNeasy mini kit (Qiagen) according to manufacturer's instructions. DNA was removed by 2h treatment with TURBO DNase (Thermo Fisher Scientific) and RNA was cleaned up using RNA Clean & Concentrator-25 kit according to the supplier’s instructions (Zymo Research). Library prep was performed according to TruSeq® Stranded mRNA kit (Illumina) protocol.
description
T-cell childhood acute lymphocytic leukemia
disease
wild type genotype
genotype
not collected
geographic location (country and/or sea)
none
infect
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
blood
organism part
3
replicate
Homo sapiens
scientific_name
JU_AU_3
title