E-MTAB-15190:MPNST1 - scNaUMI-Seq - PromethION
2025-08-27
ENA first public
ERC000011
ENA-CHECKLIST
GenomiqueENS, Institut de Biologie de l'ENS (IBENS), Département de biologie, École normale supérieure, CNRS, INSERM, Université PSL
INSDC center name
public
INSDC status
ERS24815723
SRA accession
E-MTAB-15190:MPNST1 - scNaUMI-Seq - PromethION
Submitter Id
3 to 6
month
age
ArrayExpress
broker name
tumor cell
cell type
not collected
collection date
house mouse
common name
Protocols: Nanopore sequencing libraries were prepared with the Single Cell sequencing on Promethion protocol (Nanopore) with the SQK-PCS11 kit. We re-amplified 10 ng of the 10x Genomics PCR product for 4 cycles with 5′-CAGCTTTCTGTTGGTGCTGATATTGCAAGCAGTGGTATCAACGCAGAG-3′ and 5′ Biotine-CAGACACTTGCCTGTCGCTCTATCTTCCTACACGACGCTCTTCCGATCT 3′. After 0,8x AmpureXP purification to remove excess biotinylated primers, biotinylated is bound to Dynabeads™ M-280 Streptavidin beads (Invitrogen) and amplified with the primers cPRM for 4 cycles.
description
allografted MPNST
developmental stage
Prss56cre/+, R26mt/+, NF1flox/flox
genotype
not collected
geographic location (country and/or sea)
Mouse ID 2020_23
individual
not applicable
isolate
scNaUMI-Seq
library
Mus musculus
organism
Mus musculus
organism
tumor
organism part
Mus musculus
scientific_name
PromethION (long-reads)
sequencing platform
male
sex
C57BL/6J
strain
MPNST1 - scNaUMI-Seq - PromethION
title