2025-08-28
ENA first public
ERC000011
ENA-CHECKLIST
Princess Maxima Center for Pediatric Oncology
INSDC center name
public
INSDC status
ERS24550395
SRA accession
E-MTAB-15147:Sample_tumor_HTO_batch_2
Submitter Id
ArrayExpress
broker name
WA09
cell line
inferred
cell type
not collected
collection date
human
common name
Protocols: We performed single cell dissociation using the Neural Tissue Dissociation Kit (P) (Miltenyi Biotec, Cat. #130-092-628), adjusted for DMGOs. After cutting the organoids into smaller pieces and adding the enzyme mixes, the samples were incubated on an orbital shaker at 37°C and resuspended in regular intervals with a P1000 until a single cell suspension was reached, which was verified under the microscope using trypan blue to check for cell death. After dissociation, the single cell suspension was washed twice with PBS +/+ (magnesium/calcium+ 3% FBS). Next, DAPI was used as a viability dye (DAPI 1:5000). Stained single cells suspensions were filtered using a 40 µm Flowmi cell strainer and sorted by FACS on DAPI exclusion and enriched for GFP expression for tumour cells.  Nuclei acid extraction was provided by Leiden Genome Technology Center (Leiden, The Netherlands) Library generation was provided by Leiden Genome Technology Center (Leiden, The Netherlands)
description
blastula stage
developmental stage
tumor
disease
mutant
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
blastocyst
organism part
Homo sapiens
scientific_name
female
sex
Sample_tumor_HTO_batch_2
title