2025-08-28
ENA first public
ERC000011
ENA-CHECKLIST
Princess Maxima Center for Pediatric Oncology
INSDC center name
public
INSDC status
ERS24550399
SRA accession
E-MTAB-15147:Sample_SortSeq_007
Submitter Id
ArrayExpress
broker name
WA01
cell line
microglial cell
cell type
not collected
collection date
human
common name
Protocols: BrOs and DMGOs containing microglia and optionally treated with GD2 CAR T cells were dissociated 21 days after initial microglia incorporation with the Neural Tissue Dissociation Kit (P) (Miltenyi Biotec, Cat. #130-092-628), as described above for preparation of single cell RNA and tracker seq libraries. Dissociated cells, control PMPs and unexposed GD2 CAR T cells were washed and stained in FC buffer with CD3-BV421 (1:100; BD Biosciences, clone SK7) and LIVE/DEAD Fixable Near-IR Dead Cell Stain (1:1000; Thermo Fisher) for 30 min at 4˚C. CD3+ T cells and mScarlet+ microglia/ PMP were sorted into 386-well plates containing well-specific barcoded primers (Single Cell Discoveries), one cell per well, on a Sony SH800s (Sony Biotechnology). Plates containing sorted cells were immediately snap frozen on dry ice and processed for SORT-seq by Single Cell Discoveries.  Nuclei acid extraction was provided by Single Cell Discoveries (Utrecht, Netherlands)  Library generation was provided by Leiden Genome Technology Center (Leiden, The Netherlands) Library generation was provided by Single Cell Discoveries (Utrecht, Netherlands)
description
blastula stage
developmental stage
normal
disease
wild type genotype
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
blastocyst
organism part
Homo sapiens
scientific_name
male
sex
Sample_SortSeq_007
title