2025-08-28
ENA first public
ERC000011
ENA-CHECKLIST
SAMEA110587003
External Id
Research Institute for Farm Animal Biology (FBN) Institute of Genome Biology
INSDC center name
2022-08-09T15:47:58Z
INSDC last update
public
INSDC status
ERS12684785
SRA accession
E-MTAB-12071:813
Submitter Id
70
day
age
ArrayExpress
broker name
house mouse
common name
Protocols: Tissue samples were removed, weighed, snapped frozen in liquid nitrogen, and stored at -70°C. Total RNA was isolated using TRI reagent (Sigma-Aldrich, Taufkirchen, Germany). DNA traces were removed by DNaseI-treatment (Baseline-Zero DNase; Biozym, Hessisch Oldendorf, Germany), and the RNA was cleaned-up using the RNA Clean&Concentrator-25 Kit (Zymo Research, Freiburg, Germany). RNA quantity was determined using a Qubit fluorometer (ThermoFisher Scientific, Germany), and integrity was checked on an Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA). 1 µg of total RNA with RIN > 8 was used for library preparation using a TruSeq Stranded mRNA Kit with 11 cycles of PCR-amplification according to the manufacture’s recommendation (Illumina).
description
adult
developmental stage
normal
disease
wild type genotype
genotype
liver
individual
not applicable
isolate
Mus musculus
organism
Mus musculus
organism
Mus musculus
scientific_name
male
sex
DUC
strain
813
title