2025-08-28
ENA first public
ERC000011
ENA-CHECKLIST
Princess Maxima Center for Pediatric Oncology
INSDC center name
public
INSDC status
ERS24550386
SRA accession
E-MTAB-15147:Sample_Tcells_batch_3_rxn_1
Submitter Id
ArrayExpress
broker name
primary cell line
cell line
T cell
cell type
not collected
collection date
human
common name
Protocols: DMGOs treated with GD2 CAR T cells were dissociated 14 or 35 days after initial T cell addition with the Neural Tissue Dissociation Kit (P) (Miltenyi Biotec, Cat. #130-092-628), as described above for preparation of single cell RNA and tracker seq libraries. Dissociated cells were washed and stained in FC buffer with CD3-APC (1:80; BD Biosciences, clone SK7) and LIVE/DEAD Fixable Near-IR Dead Cell Stain (1:1000; Thermo Fisher) for 30 min at 4˚C. CD3+ T cells and GFP+ tumour cells were sorted on a CytoFLEX SRT Benchtop Cell Sorter (Beckman Coulter) and immediately processed for scRNA-seq.   Library generation was provided by Leiden Genome Technology Center (Leiden, The Netherlands)
description
adult
developmental stage
normal
disease
wild type genotype
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
blood
organism part
Homo sapiens
scientific_name
unknown
sex
Sample_Tcells_batch_3_rxn_1
title