2025-08-29
ENA first public
ERC000011
ENA-CHECKLIST
University of Cambridge Department of Pathology
INSDC center name
public
INSDC status
ERS26530897
SRA accession
E-MTAB-15534:M Ribo
Submitter Id
ArrayExpress
broker name
iBMDM
cell line
macrophage
cell type
not collected
collection date
house mouse
common name
Protocols: iBMDM were infected with WT or ΔprgJ Salmonella Typhimurium or mock infected for 60 min. Cells were lysed in buffer containing cycloheximide and chloramphenicol, and lysates were split for RNA-Seq and Ribo-Seq. For Ribo-Seq, lysates were treated with RNase I and fragments protected from digestion by the ribosome were purified. For RNA-Seq, total cellular RNA was fragmented by alkaline hydrolysis. Both produce short RNA fragments, from which libraries were generated.
description
adult
developmental stage
normal
disease
wild type genotype
genotype
not collected
geographic location (country and/or sea)
Mock
infect
not applicable
isolate
Mus musculus
organism
Mus musculus
organism
Bone marrow derrived macrophage
organism part
Mus musculus
scientific_name
M Ribo
title