2025-08-30
ENA first public
ERC000011
ENA-CHECKLIST
SAMEA114236723
External Id
Department of Pathology, University of Cambridge
INSDC center name
2023-08-04T10:26:00Z
INSDC last update
public
INSDC status
ERS16222163
SRA accession
E-MTAB-13212:Sample 8
Submitter Id
ArrayExpress
broker name
immortalised bone marrow derived macrophage
cell_line
macrophage
cell_type
not collected
collection date
house mouse
common name
Protocols: A clonal population of immortalised bone marrow derrived macrophages following CRISPR KO of Egr1 or CRISPR against no target (i.e. WT) were infected with Salmonella enterica sv. Typhimurium (strain SL1344) at a multiplicity of infection of 10 or mock-infected. Gentamicin was added to cultures at 15 min post infection to kill extracellular bacteria. RNA was extracted from cultured cells at the indicated time point by Trizol purification according to manufacturer's instructions Libraries were generated with the Lexogen QuantSeq 3' mRNA-Seq Library Prep Kit FWD according to manufacturer's instructions
description
adult
developmental stage
normal
disease
EGR1 knockout
genotype
not collected
geographic location (country and/or sea)
mock
infect
not applicable
isolate
Mus musculus
organism
Mus musculus
organism
bone marrow
organism part
Mus musculus
scientific_name
Sample 8
title