2025-08-30
ENA first public
ERC000011
ENA-CHECKLIST
Institute of Human Genetics Polish Academy of Sciences
INSDC center name
public
INSDC status
ERS23783951
SRA accession
E-MTAB-14919:Y01966_PA_P4
Submitter Id
9
year
age
P4
batch
ArrayExpress
broker name
prior to treatment
clinical history
not collected
collection date
human
common name
Protocols: Leukemic T-cells were obtained from bone marrow samples by isolation of mononuclear cells, using density gradient centrifugation, followed by immunomagnetic separation, with use of Human T Lymphocyte Enrichment Set-DM (Becton Dickinson) in case of samples with blast percentage below 85%. Thymocyte CD4+ CD8+ and CD34+ samples, obtained from children undergoing cardiac surgery (UZ Gent), were used as controls. Immature CD34+ thymocytes were purified based on MACS purification using CD34 microbeads (Miltenyi Biotec) without lineage depletion, while CD4 and CD8 labeling was used to sort the CD4+ CD8+ double-positive subset by a FACSAriaIII (BDBiosciences). The purity of each subset was at least 98%. Total RNA was isolated using miRNeasy Kit (Qiagen). RNA isolates were DNase treated and purified with use of RNA Clean and Concentrator Kit (Zymo Research). Libraries were generated with Illumina TruSeq Stranded mRNA Prep Kit. Modified fragmentation conditions were used (90'C for 2 minutes)
description
juvenile stage
developmental stage
T-cell acute lymphoblastic leukemia
disease
not collected
geographic location (country and/or sea)
Y01966
individual
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
bone marrow
organism part
Homo sapiens
scientific_name
female
sex
Y01966_PA_P4
title