2025-08-30
ENA first public
ERC000011
ENA-CHECKLIST
Department of Microbiology, Faculty of Science, Mahidol University
INSDC center name
public
INSDC status
ERS25301607
SRA accession
E-MTAB-15328:Sample 3
Submitter Id
ArrayExpress
broker name
mononuclear cell
cell type
Convalescence
clinical information
not collected
collection date
human
common name
Protocols: Frozen peripheral blood mononuclear cells (PBMCs) were thawed at 37°C and diluted five times 1:1 in RPMI containing 10% FBS, following the 10x Genomics protocol for fresh frozen human PBMCs. Dead cells were removed using a Dead Cell Removal Kit (Miltenyi Biotec), and cell clumps were filtered with 40 µm Flowmi® strainers (SP Industries). Cell counts were determined microscopically prior to single-cell capture. No separate RNA extraction was performed. Single-cell suspensions were directly loaded into the 10x Chromium™ Controller for gel bead-in-emulsion (GEM) generation and reverse transcription. Reverse transcription, cDNA amplification, and library construction were performed using Chromium™ Single Cell V(D)J Reagent Kits with V1 Chemistry (10x Genomics), following the manufacturer’s protocol. Target recovery was 10,000 cells per sample without sample multiplexing.
description
Dengue Hemorrhagic Fever
disease
Dengue Hemorrhagic Fever
disease staging
not collected
geographic location (country and/or sea)
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
blood
organism part
Homo sapiens
scientific_name
Sample 3
title