2025-08-31
ENA first public
ERC000011
ENA-CHECKLIST
Departamento de Producción Animal
INSDC center name
public
INSDC status
ERS25250595
SRA accession
E-MTAB-15357:Sample 16
Submitter Id
31
day
age
ArrayExpress
broker name
not collected
collection date
sheep
common name
Protocols: At the slaughter, immediately after the sacrifice, samples from the perirenal and tail adipose tissues were taken from each of the eight animals. Both tissues were kept for 24 hours at 4°C in an RNA-stabilization solution (Ambion RNAlater; Life Technologies). Following the removal of the RNA-stabilization solution, the samples were stored at -80°C until the extraction of RNA. These lambs were reared at the commercial flock of the Instituto Ganadero de Montaña (IGM; Grulleros, León, Castile and León, Spain). All were male lambs born in the same lambing season (January-February 2020). The lambs were reared under the same management and feeding conditions. After birth and receiving the colostrum from their dams, lambs were fed ad libitum with a milk replacer (Ovilac 60; Calfvet®). The animals were sacrificed once they reached a body weight between 10 and 12.5 kg in vivo weight, and a maximum age of 35 days. RNA extraction was carried out for the chosen samples using the miRNeasy Mini Kit from Qiagen® adjusted for use with adipose tissue. Using an Agilent 2100 Bioanalyzer (Agilent Technologies) to evaluate the RNA integrity value of the samples, values greater than 7.2 were obtained for every sample. Following the acquisition of RNA, the cDNA libraries were constructed using the UltraTM RNA Library Prep Kit (NEBNext®) by Novogene (Cambridge, United Kingdom). A minimum of 0.8 μg of RNA per sample was used to prepare a total of 16 stranded paired-end libraries.
description
Suckling Lamb
developmental stage
wild type genotype
genotype
not collected
geographic location (country and/or sea)
69
individual
not applicable
isolate
Ovis aries
organism
Ovis aries
organism
tail adipose tissue
organism part
Ovis aries
scientific_name
male
sex
Assaf
strain
Sample 16
title