2025-09-01
ENA first public
ERC000011
ENA-CHECKLIST
NMBU
INSDC center name
public
INSDC status
ERS22820738
SRA accession
E-MTAB-14732:R2SNK
Submitter Id
1
year
age
ArrayExpress
broker name
not collected
collection date
timothy grass
common name
Noreng
cultivar
Protocols: Plants from all temporal seedlots of Engmo and Norneg were cold acclimated under ambient light conditions, first for 6 weeks at 9°C, followed by 2 weeks at 6°C and finally 2 weeks at 2°C under 12h light ( 55 PPFD). Freezing stress samples were transfered to freezers the temperature was lowered from 2°C to -3°C by 1°C h−1 and were kept at -3°C for ~ 12 hrs to avoid supercooling. After that the temperatures  were lowered by 1°C h−1 from -3°C to -10°C and by 3°C until the pre-set temperatures were reached, i.e. -12, -15, -18, -21, -24, -27°C. batches of samples were taken at the predetermined temperatures flash-frozen for RNA extraction. The ice encasement samples were placed in a polyethyene container and filled with ice and cold water and kept in freezing rooms and batches of plants were withdrawn at 22,36, 54, 68, 75 and 82 days. The sampled plant material from freezing and ice encasement tests were crushed with mortar and pistel and the ground plant material is used for RNA extraction does not apply RNA extraction was carried out using Qiagen RNeasy Plant Mini kit according to manufacturar guidelines The libraries were prepared according to the TruSeq RNA Sample Prep Kit v2 (Illumina, San Diego, CA, USA)
description
11 week seedling
developmental stage
not collected
geographic location (country and/or sea)
not applicable
isolate
Phleum pratense
organism
Phleum pratense
organism
Crown tissue
organism part
Current
population
2
replicate
Phleum pratense
scientific_name
Cold acclimation
stimulus
Control
stress
R2SNK
title