2025-09-01
ENA first public
ERC000011
ENA-CHECKLIST
LIV
INSDC center name
public
INSDC status
ERS20622344
SRA accession
E-MTAB-14300:14-AORTA-YOUNG-4
Submitter Id
3
month
age
ArrayExpress
broker name
not collected
collection date
black rat
common name
Protocols: Rats were euthanised by a Schedule one procedure and the cardiac left ventricle dissected and snap frozen in RNALater. Naturally Ageing Aging Natural Ageing Aging RNA extraction was performed using the RNeasy Fibrous Tissues kit (Qiagen, UK) as per manufacturer’s instructions. Approx. 30mg of tissue was lysed and homogenised using an ultra turrax (IKA Homogeniser, Sigma, UK) in buffer RLT before proteinase K digestion steps, total RNA bound followed by several washing steps and DNase digestion and total RNA eluted. Total RNA integrity was confirmed using the Bioanalyzer (Agilent Technologies, USA). Ribosomal RNA (rRNA) was depleted from RNA samples by using the Ribo-Zero™ rRNA Removal Kit (Epicentre, USA) in accordance with the instructions of the manufacturer. NEBNext® Ultra II Directional RNA Library Prep Kit for Illumina, so they are paired-end reads but the orientation of the RNA strand is preserved in the first strand
description
Young Adult
developmental stage
wild type genotype
genotype
not collected
geographic location (country and/or sea)
24
individual
not applicable
isolate
Rattus rattus
organism
Rattus rattus
organism
aorta
organism part
Rattus rattus
scientific_name
male
sex
Wistar Kyoto
strain
14-AORTA-YOUNG-4
title