2025-09-01
ENA first public
ERC000011
ENA-CHECKLIST
OXFORDUNIV
INSDC center name
public
INSDC status
ERS22717052
SRA accession
E-MTAB-14722:S8-14wk-DIP
Submitter Id
14
week
age
ArrayExpress
broker name
not collected
collection date
human
common name
Protocols: Skin and tendon material were removed from each finger, and DIP and PIP joints isolated by cutting either side of the joint space. Joints were cut into pieces and enzymatically digested with 0.1mg/ml of liberaseTL in DMEM, at 37 degrees C for 1h 30mins. Supernatant containing released cells was collected every 10-15 mins, neutralized in DMEM 10% FBS, and stored at 4 degrees C. Once supernatant was removed, fresh enzyme was applied to continue sample digestion. After digestion, the remaining tissue was manually dissociated, and combined with collected supernatants, in DMEM 10% FBS. All isolated cells were then passed through a 70μm filter. Where possible, cells were sorted for viability using the 7-AAD dye (Biolegend 420403) and loaded onto the 10X Genomics Chromium Controller (Chip G) following the manufacturer’s protocol to capture 10,000 cells per reaction.  As per 10x Genomics Single Cell 3' Reagent Kits v3.1 manufacturer user guide (CG000330 Rev B) As per 10x Genomics Single Cell 3' Reagent Kits v3.1 manufacturer user guide (CG000330 Rev B)
description
embryo stage
developmental stage
3
digestion protocol
not collected
geographic location (country and/or sea)
S8
individual
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
distal interphalangeal finger joint
organism part
late_joint_development
phenotype
embryo_14wk_finger_DIP_R1
sample_id
Homo sapiens
scientific_name
female
sex
S8-14wk-DIP
title