2025-09-01
ENA first public
ERC000011
ENA-CHECKLIST
Columbia University Irving Medical Center (CUIMC), Aaron Diamond AIDS Research Center
INSDC center name
public
INSDC status
ERS22719188
SRA accession
E-MTAB-14727:MKH-FB
Submitter Id
ArrayExpress
broker name
N/A
cell_type
not collected
collection date
human
common name
Protocols: HLA-A2+/peptide pentamer and tetramer selection from patient PBMCs 10x Genomics 3' v3.1 protocol. In brief, after cell sorting, single-cell suspensions were loaded into the Chromium controller to make nanoliterscale droplets with uniquely barcoded 3’ gel beads called GEMs (gel bead-in emulsions). After GEM-RT, GEMs were cleaned up by Dyna beads MyOne Silane beads (Thermo Fisher Scientific, 37002D). According to the manufacturer’s instructions, the cDNA was amplified and size-selected by SPRI-beads (Beckman Coulter, B23317). Finally, the 3’ transcript and feature barcode libraries were pooled 10x Genomics 3' v3.1 protocol. In brief, after cell sorting, single-cell suspensions were loaded into the Chromium controller to make nanoliterscale droplets with uniquely barcoded 3’ gel beads called GEMs (gel bead-in emulsions). After GEM-RT, GEMs were cleaned up by Dyna beads MyOne Silane beads (Thermo Fisher Scientific, 37002D). According to the manufacturer’s instructions, the cDNA was amplified and size-selected by SPRI-beads (Beckman Coulter, B23317). Finally, the 3’ transcript and feature barcode libraries were pooled
description
not available
developmental stage
normal
disease
not collected
geographic location (country and/or sea)
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
N/A
organism part
Homo sapiens
scientific_name
not available
sex
MKH-FB
title