2025-09-01
ENA first public
ERC000011
ENA-CHECKLIST
The University of Manchester
INSDC center name
public
INSDC status
ERS25198417
SRA accession
E-MTAB-15344:Sample 4
Submitter Id
6
month
age
ArrayExpress
broker name
not collected
collection date
house mouse
common name
Protocols: Mice were PBS perfused and right (ipsilateral) hemispheres were extracted and homogenised using lysing matrix D tubes (MPBio) in a Qiagen Tissue Lyser II. A model of intraparenchymal brain haemorrhage was used that created an active bleed within the brain by using collagenase VII-S (Sigma, C2399) to break down the basal lamina of cerebrovascular beds. To do this, mice were initially induced under anaesthesia using 4 % isoflurane in 70 % N2O and 30 % O2 and fur was shaven from the head. Mice were then transferred to a feedback-controlled heating pad set to 37 ˚C, securely fixed to a stereotactic frame (Stoetling), anaesthesia maintained at 1.5-2.0 % isoflurane in 30 % N2O and 70 % O2 using a nose cone, and Videne (EcoLab) applied to the scalp. A longitudinal midline incision above the skull was performed and periosteum stripped away from the midline on both sides. A burr-hole was created using a micro-drill and a glass micropipette (BLAUBRAND, pulled at 70 ˚C) inserted at the following co-ordinates from bregma: anterior-posterior 0.0 mm, lateral -2.0 mm, deep -2.7 mm. 0.5 µL of 0.09 units µL-1 of collagenase dissolved in saline was then injected at a rate of 1 µL min-1. The needle was left in situ for 10 min before removal and wounds sutured. The scalp was cleaned once more with Videne and a topical analgesia was applied (EMLA cream, AstraZeneca) before animals were given a subcutaneous bolus of saline (10 mL kg-1) and buprenorphine (50 µg kg-1, Vetergesic, UK) before being recovered in 28 ˚C housing. Animals were then transferred to ventilated cages suspended over a heating pad with free access to mashed food and water in normal housing conditions. RNA was isolated using the RNeasy lipid tissue mini kit (Qiagen) as per manufacturer’s protocol. RNA-Seq libraries were generated using the TruSeq Stranded mRNA assay (Illumina, Inc.) according to the manufacturer’s instructions.
description
adult
developmental stage
wild type genotype
genotype
not collected
geographic location (country and/or sea)
7
individual
ICH
injury
not applicable
isolate
Mus musculus
organism
Mus musculus
organism
cerebral hemisphere
organism part
Mus musculus
scientific_name
male
sex
C57BL/6
strain
Sample 4
title