2025-09-01
ENA first public
ERC000011
ENA-CHECKLIST
1. Univ. Lille, CNRS, Inserm, CHU Lille, Institut de Recherche contre le Cancer de Lille, UMR9020 – UMR-S 1277 – Canther Cancer Heterogeneity, Plasticity and Therapy Resistance F-59000 Lille, France  2. Banque de Tissus, Centre de biologie-pathologie, CHU Lille, F-59000 Lille France
INSDC center name
public
INSDC status
ERS25250616
SRA accession
E-MTAB-15359:K562_D15
Submitter Id
ArrayExpress
broker name
K562
cell line
myeloid cell
cell type
not collected
collection date
human
common name
Protocols: Cell lines were analyzed after 15 days of culture in suspension. Cells within GelMA 5% hydrogels were collected after dissociation with collagenase type I. All samples were stored at −80 °C until RNA extraction. Total RNA was extracted using the Quick-RNA Microprep Kit (Zymo Research, Irvine, CA, USA) according to the manufacturer’s protocol. RNA quantity and purity were assessed with a NanoDrop spectrophotometer (260/280 and 260/230 ratios), and RNA integrity was evaluated using an Agilent 2100 Bioanalyzer with an RNA 6000 Nano Kit. Only samples with RNA Integrity Number (RIN) greater than 7 were retained for library preparation RNA libraries were prepared using the AccuraCode RNA-Seq Kit (Cat. no. 10710174, Singleron Biotechnologies GmbH) following the manufacturer’s instructions. Polyadenylated mRNA was isolated using AccuraCode beads (Cat. no. 1200030080). cDNA was synthesized with sample-specific barcodes and unique molecular identifiers (UMIs), pooled, and amplified to construct sequencing libraries.
description
adult
developmental stage
myeloid sarcoma
disease
not collected
geographic location (country and/or sea)
GelMA 5% hydrogel
growth condition
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
Homo sapiens
scientific_name
K562_D15
title