2025-09-02
ENA first public
ERC000011
ENA-CHECKLIST
UNIVERSITY OF SZEGED
INSDC center name
public
INSDC status
ERS22560239
SRA accession
Canfam_stool_AK_LPL_P_PM2_230812_Zold4
Submitter Id
5’-TTTTTTTTCCTGTACTTCGTTCAGTTACGTATTGCT-3’
adapters
breeder's line
biological status
Transdanubian Mountains, Pannonian Basin
broad-scale environmental context
2023-08-12
collection date
dog
common name
Dog stool sample of Zold
description
Hungary
geographic location (country and/or sea)
46.409100
DD
geographic location (latitude)
18.567699
DD
geographic location (longitude)
Zomba
geographic location (region and locality)
NA
gravidity
10,00
weeks
host age
stool
host body product
Hungarian herding terrier: Pumi
host common name
Royal Canin Starter and Real Nature Junior Rind; Pferd and rabbit ears
host diet
Puppy of Guba, Litter P, Le Patit Lapin kennel
host family relationship
puppy
host life stage
Canis lupus familiaris
host scientific name
male
host sex
LPL_P_Zold
host subject id
cross-breeding of Hungarian Puli with French and German herding dogs originated from 17. century
host subspecific genetic lineage
9615
host taxid
16S Barcoding Kit (SQK-16S024)
library construction method
AMPLICON
library screening strategy
1500 bp
library size
garden/house
local environmental context
ACCACTGCCATGTATCAAAGTACG
multiplex identifiers
LongAmp Hot Start Taq 2X Master Mix (NEB, M0533L)
nucleic acid amplification
ZymoBIOMICS 96 MagBead DNA Kit-Dx (cat # D4308-E)
nucleic acid extraction
Canis lupus familiaris
organism
Initial denaturation 1 min 95 Â°C (1 cycle); Denaturation 20 secs 95 Â°C (25 cycles); Annealing 30 secs 55 Â°C (25 cycles); Extension 2 mins 65 Â°C (25 cycles); Final extension 5 mins 65 Â°C (1 cycle)
pcr conditions
27F 5'-AGAGTTTGATCCTGGCTCAG-3'; 1492R 5'-CGGTTACCTTGTTACGACTT-3'
pcr primers
Metagenomic Landscape of the Canine Gut Microbiome Using Full-Length 16S rRNA Gene Sequencing
project name
Eppendorf_CryoCube_F740h
sample storage location
-80
Â°C
sample storage temperature
0,75
mL
sample volume or weight for DNA extraction
Canis lupus familiaris
scientific_name
16S rRNA
target gene
V1-V9
target subfragment
23
ÂşC
temperature
Dog_gut_microbiome_LPL_P_PM2_230812_Zold4
title