2025-09-02
ENA first public
ERC000011
ENA-CHECKLIST
Institute of Plant Genetics, Polish Academy of Sciences
INSDC center name
public
INSDC status
ERS24612001
SRA accession
E-MTAB-15161:Sample 27
Submitter Id
ArrayExpress
broker name
not collected
collection date
Helos
cultivar
Protocols: After co-cultivation of Agrobacteria and A. tumefaciens, the cells were collected at different time points (0.5 h, 3 h, 12 h, 24 h). After the cells were vacuum filtered from the medium, they were immediately cryopreserved in liquid nitrogen and stored at -80 °C.  Three biological replicates for the treatment and control were incubated in a growth chamber with a photoperiod of 16 hours of light and 8 hours of darkness at 25 °C, an irradiation of 80 µmol m-²s-¹ and a relative humidity of 70, and both the treated and control cultures were kept alive. A. tumefaciens or A. rhizogenes was added to H. perforatum cell suspension cultures in the exponential phase (three to five days after subculture).  Total RNA was isolated from 100 mg of biomass using the Sigma Spectrum RNA extraction kit (Sigma-Aldrich, USA). RNA samples with an RNA integrity number (RIN) of at least seven were used for RNAseq analysis.  Random hexamer primers were used to synthesise the cDNA after random fragmentation of the mRNA. DNA polymerase I, dNTPs, RNase H and a special second-strand synthesis buffer (Illumina) were used to generate the second strand.
description
not collected
geographic location (country and/or sea)
not applicable
isolate
Hypericum perforatum
organism
Hypericum perforatum
organism
plant cell suspension culture
organism part
1
replicate
Hypericum perforatum
scientific_name
Sample 27
title
Control
treatment