2025-09-02
ENA first public
ERC000011
ENA-CHECKLIST
Université Paris Cité, INSERM, CNRS, Institut Cochin
INSDC center name
public
INSDC status
ERS21389958
SRA accession
E-MTAB-14570:Sample 11
Submitter Id
adult
age
ArrayExpress
broker name
RS
cell_type
not collected
collection date
house mouse
common name
Protocols: Primary spermatocytes (SC1), secondary spermatocytes (SC2), and round spermatid (RS) cells were isolated from wild type (XY), Zfy1 KO, Zfy2 KO, and Zfy DKO mice via germ cell isolation and fluorescence-activated cell sorting (FACS). RNA was extracted from each SC1, SC2, and RS cell fraction using the RNeasy Micro Kit (Qiagen, Hilden, Germany). The cDNA libraries were prepared using the NEBNext® UltraTM II Directional RNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA) before quality assessment with the Agilent Bioanalyzer.
description
adult
developmental stage
XY (wild type)
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Mus musculus
organism
Mus musculus
organism
testis
organism part
Mus musculus
scientific_name
male
sex
C57BL/6J
strain
Sample 11
title