2025-09-02
ENA first public
ERC000011
ENA-CHECKLIST
UNIVERSITY OF SZEGED
INSDC center name
public
INSDC status
ERS22560326
SRA accession
Canfam_stool_AK_RR_0_AF2_230212_Hanga8
Submitter Id
5’-TTTTTTTTCCTGTACTTCGTTCAGTTACGTATTGCT-3’
adapters
breeder's line
biological status
Transdanubian Mountains, Pannonian Basin
broad-scale environmental context
2023-02-12
collection date
dog
common name
Dog stool sample of Hanga
description
Hungary
geographic location (country and/or sea)
47.106880
DD
geographic location (latitude)
17.563959
DD
geographic location (longitude)
Ajka
geographic location (region and locality)
gravid (49 post-conception day)
gravidity
138,29
weeks
host age
stool
host body product
Hungarian herding terrier: Pumi
host common name
Gosbi Grain Free Adult
host diet
Mother of Litter F, Rezerta Reti kennel
host family relationship
adult
host life stage
Canis lupus familiaris
host scientific name
female
host sex
RR_0_Hanga
host subject id
cross-breeding of Hungarian Puli with French and German herding dogs originated from 17. century
host subspecific genetic lineage
9615
host taxid
16S Barcoding Kit (SQK-16S024)
library construction method
AMPLICON
library screening strategy
1500 bp
library size
garden/house
local environmental context
GCATAGTTCTGCATGATGGGTTAG
multiplex identifiers
LongAmp Hot Start Taq 2X Master Mix (NEB, M0533L)
nucleic acid amplification
ZymoBIOMICS 96 MagBead DNA Kit-Dx (cat # D4308-E)
nucleic acid extraction
Canis lupus familiaris
organism
Initial denaturation 1 min 95 Â°C (1 cycle); Denaturation 20 secs 95 Â°C (25 cycles); Annealing 30 secs 55 Â°C (25 cycles); Extension 2 mins 65 Â°C (25 cycles); Final extension 5 mins 65 Â°C (1 cycle)
pcr conditions
27F 5'-AGAGTTTGATCCTGGCTCAG-3'; 1492R 5'-CGGTTACCTTGTTACGACTT-3'
pcr primers
Metagenomic Landscape of the Canine Gut Microbiome Using Full-Length 16S rRNA Gene Sequencing
project name
Eppendorf_CryoCube_F740h
sample storage location
-80
Â°C
sample storage temperature
0,75
mL
sample volume or weight for DNA extraction
Canis lupus familiaris
scientific_name
16S rRNA
target gene
V1-V9
target subfragment
4
ÂşC
temperature
Dog_gut_microbiome_RR_0_AF2_230212_Hanga8
title