2025-09-02
ENA first public
ERC000011
ENA-CHECKLIST
UNIVERSITY OF SZEGED
INSDC center name
public
INSDC status
ERS22560320
SRA accession
Canfam_stool_AK_RR_0_AF1_240318_Anna10
Submitter Id
5’-TTTTTTTTCCTGTACTTCGTTCAGTTACGTATTGCT-3’
adapters
breeder's line
biological status
Transdanubian Mountains, Pannonian Basin
broad-scale environmental context
2024-03-18
collection date
dog
common name
Dog stool sample of Anna
description
Hungary
geographic location (country and/or sea)
47.106880
DD
geographic location (latitude)
17.563959
DD
geographic location (longitude)
Ajka
geographic location (region and locality)
non gravid (5 post-parturition day)
gravidity
273,86
weeks
host age
stool
host body product
Hungarian herding terrier: Pumi
host common name
Gosbi Grain Free Adult + boiled chicken and pasta
host diet
Mother of Litter G, Rezerta Reti kennel
host family relationship
adult
host life stage
Canis lupus familiaris
host scientific name
female
host sex
RR_0_Anna
host subject id
cross-breeding of Hungarian Puli with French and German herding dogs originated from 17. century
host subspecific genetic lineage
9615
host taxid
16S Barcoding Kit (SQK-16S024)
library construction method
AMPLICON
library screening strategy
1500 bp
library size
garden/house
local environmental context
AGAACGACTTCCATACTCGTGTGA
multiplex identifiers
LongAmp Hot Start Taq 2X Master Mix (NEB, M0533L)
nucleic acid amplification
ZymoBIOMICS 96 MagBead DNA Kit-Dx (cat # D4308-E)
nucleic acid extraction
Canis lupus familiaris
organism
Initial denaturation 1 min 95 Â°C (1 cycle); Denaturation 20 secs 95 Â°C (25 cycles); Annealing 30 secs 55 Â°C (25 cycles); Extension 2 mins 65 Â°C (25 cycles); Final extension 5 mins 65 Â°C (1 cycle)
pcr conditions
27F 5'-AGAGTTTGATCCTGGCTCAG-3'; 1492R 5'-CGGTTACCTTGTTACGACTT-3'
pcr primers
Metagenomic Landscape of the Canine Gut Microbiome Using Full-Length 16S rRNA Gene Sequencing
project name
Eppendorf_CryoCube_F740h
sample storage location
-80
Â°C
sample storage temperature
0,75
mL
sample volume or weight for DNA extraction
Canis lupus familiaris
scientific_name
16S rRNA
target gene
V1-V9
target subfragment
6
ÂşC
temperature
Dog_gut_microbiome_RR_0_AF1_240318_Anna10
title