E-MTAB-15402:Sample 3
2025-09-05
ENA first public
ERC000011
ENA-CHECKLIST
UMASS Medical School
INSDC center name
public
INSDC status
ERS25320471
SRA accession
E-MTAB-15402:Sample 3
Submitter Id
ArrayExpress
broker name
not collected
collection date
house mouse
common name
Protocols: Wild-type (CNBP vav-iCre -/- ) and  knockout (CNBP vav-iCre +/+) mice were uninfected (0 days) or infected for 3,5,7,and 9 days with Plasmodium chabaudi. Infected mice were injected intraperitoneally with 1 x 10^6 Plasmodium chabaudi-infected red blood cells, while uninfected controls received an injection of sterile 1X PBS. All mice were euthanized at the specified day and spleens were harvested. C57BL/6 mice (Jackson Laboratory, Bar Harbor, ME) were maintained in specific pathogen-free conditions and used in accordance with the Institutional Animal Care and Use Committee.  Intra-peritoneal injection of 1 x10e6 P.chabaudi infected Red Blood Cells. Approximately 15 mg of splenic tissue was homogenized in RLT buffer from the RNeasy Kit (Qiagen) using a TissueLyser at 25 Hz for a total of 2 minutes, with a 30-second incubation on ice between cycles. The homogenates were centrifuged at 10,000 g for 10 min at 4C  to remove cells and debris, and the Qiagen RNeasy Kit protocol was followed for RNA extraction. On-column DNase treatment was included during RNA isolation. RNA integrity was assessed using a Fragment Analyzer. 1ug total RNA was used on magnetic bead for capture of polyA mRNA.   Illumina Stranded mRNA Prep Kit was used for library preparation according to manufacturers protocol. All samples were sized, quantified, and validated on a Bioanalyzer to ensure high quality for sequencing.
description
adult
developmental stage
CNBP F/F x VAV- iCre -/-
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Mus musculus
organism
Mus musculus
organism
spleen
organism part
Mus musculus
scientific_name
male
sex
C57BL/6J
strain
Sample 3
title