E-MTAB-13602:N3a-2
2025-09-05
ENA first public
ERC000011
ENA-CHECKLIST
SAMEA114786187
External Id
Institute of Molecular Oncology, Philipps-University Marburg
INSDC center name
2023-12-03T21:27:34Z
INSDC last update
public
INSDC status
ERS17207564
SRA accession
E-MTAB-13602:N3a-2
Submitter Id
ArrayExpress
broker name
U2OS
cell_line
epithelial cell
cell_type
not collected
collection date
human
common name
Protocols: HeLa and U2OS cells were transfected with pcDNA3.1(+) plasmids containing the respective DARPin using Lipofectamin 2000 transfection reagent (Thermo Fisher Scientific) according to the manufacturer’s instructions. Twenty-four hours after transfection cells were washed with PBS, detached and mRNA was isolated using the RNease mini kit (Qiagen). Cells were harvested and RNA was isolated using the RNeasy Mini Kit (QIAGEN, 74106) according to manufacturer’s protocol. RNA was isolated using the RNeasy Mini kit (Qiagen) and RNA quality was assessed using the Experion RNA StdSens Analysis Kit (Bio‐Rad). RNAseq libraries were prepared from total RNA with the Lexogen QuantSeq 3'-mRNA Library Prep Kit FWD for Illumina (Lexogen, 015.24) in combination with the UMI Second Strand Synthesis Module for QuantSeq FWD (Illumina, Read 1) (Lexogen, 081.96) following the manufacturer's protocol. Quality of sequencing libraries was controlled on a Bioanalyzer 2100 using the Agilent High Sensitivity DNA Kit (Agilent).
description
adult
developmental stage
osteosarcoma
disease
empty vector
genetic modification
wild type genotype
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
epithelium
organism part
Homo sapiens
scientific_name
N3a-2
title