2025-09-08
ENA first public
ERC000011
ENA-CHECKLIST
SAMEA115120794
External Id
Division of Cancer Sciences, The University of Manchester
INSDC center name
2024-01-19T17:23:33Z
INSDC last update
public
INSDC status
ERS17768781
SRA accession
E-MTAB-13720:BAP30T1
Submitter Id
ArrayExpress
broker name
not collected
collection date
human
common name
Protocols: Normal breast tissue cores obtained via Vacuum-Assisted Biopsy (VAB) with a 10G biopsy needle were snap frozen for RNA extraction. A baseline VAB was performed in the luteal phase of the menstrual cycle and a second VAB was performed after a 12-week anti-progestin (ulipristal acetate, daily 5mg oral tablet) treatment. RNA was extracted using the RNeasy Plus Micro Kit (#74034, Qiagen) as described in the manufacturer’s handbook. Indexed PolyA libraries were prepared using 100ng of total RNA and 15 cycles of amplification in the Agilent Sure Select Strand Specific RNA Library Prep Kit for Illumina Sequencing (Agilent Cat No: G9691B). Libraries were quantified by qPCR using a Kapa Library Quantification Kit for Illumina sequencing platforms (Kapa Biosystems Inc. Cat No: KK4873).
description
adult
developmental stage
normal
disease
not collected
geographic location (country and/or sea)
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
mammary gland
organism part
Homo sapiens
scientific_name
female
sex
BAP30T1
title