2025-09-10
ENA first public
ERC000011
ENA-CHECKLIST
University of Birmingham
INSDC center name
public
INSDC status
ERS20962388
SRA accession
E-MTAB-14434:rpoS-3xFLAG replicate 1
Submitter Id
ArrayExpress
broker name
not collected
collection date
Protocols: The Vibrio cholerae O1 El Tor strain A1552 was isolated in 1992 from a traveller from Peru. Cells were grown in liquid LB medium, at 37 degrees with shaking, to an OD600 of 2.0. Immunoprecipitations were done as described by Haycocks et al (PLoS Genet. 2019. 15, e1008362) using derivatives of Vibrio cholerae strain A1552 using anti-FLAG antibodies (Sigma).  Libraries were prepared using immunoprecipited protein-DNA complexes immobilised with Protein A-sepharose. DNA fragments were then blunt ended, A-tailed and barcoded. Following elution of complexes from the Protein-A sepharose, crosslinks were reversed and barcoded libraries were amplified by PCR. The number of PCR cycles was determined empirically for each library. After amplification, library concentration was quantified using Qubit analysis and real time PCR.
description
Cholera
disease
rpoS3xFLAG
genotype
not collected
geographic location (country and/or sea)
Stationary phase
growth condition
not applicable
isolate
Vibrio cholerae
organism
Vibrio cholerae
organism
nucleoid
organism part
Vibrio cholerae
scientific_name
A1552
strain
rpoS-3xFLAG replicate 1
title