2025-09-12
ENA first public
ERC000011
ENA-CHECKLIST
Gyeongsang National University Animal Genomics Laboratory
INSDC center name
public
INSDC status
ERS22973172
SRA accession
E-MTAB-14776:0326-Liver
Submitter Id
28
month
age
18.2
kilogram
body weight
ArrayExpress
broker name
not collected
collection date
pig
common name
Protocols: To identify tissue-specific body size-related genes, approximately 10 samples representing 5 different tissues were collected from MiniPig individuals with varying body sizes. This comprehensive sampling aimed to capture the relationship between tissue expression patterns and body size variations. All experimental procedures were thoroughly reviewed and approved by the National Institute of Animal Science (NIAS) under approval number NIAS20181295. Genomic DNA was extracted from tissue samples using the QIAamp DNA Mini Kit (Qiagen, Cat. No. 51306) following the manufacturer’s protocol. DNA concentration and purity were assessed, and only samples meeting quality standards were used for library preparation. Individual libraries were generated using the Accel-NGS Methyl-Seq DNA Library Kit (Swift BioSciences) following the manufacturer's protocol. The bisulfite-converted DNA fragments were repaired, adapter-ligated, and amplified to prepare the WGBS libraries.
description
wild type genotype
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Sus scrofa
organism
Sus scrofa
organism
liver
organism part
ET Type
phenotype
Sus scrofa
scientific_name
male
sex
0326-Liver
title