2025-09-12
ENA first public
ERC000011
ENA-CHECKLIST
Gyeongsang National University Animal Genomics Laboratory
INSDC center name
public
INSDC status
ERS22972438
SRA accession
E-MTAB-14775:0309-Kidney
Submitter Id
28
month
age
18.8
kilogram
body weight
ArrayExpress
broker name
not collected
collection date
pig
common name
Protocols: To identify tissue-specific body size-related genes, approximately 10 samples representing 5 different tissues were collected from MiniPig individuals with varying body sizes. This comprehensive sampling aimed to capture the relationship between tissue expression patterns and body size variations. All experimental procedures were thoroughly reviewed and approved by the National Institute of Animal Science (NIAS) under approval number NIAS20181295. Total RNA was extracted from tissue samples using the QIAzol® Lysis Reagent (Qiagen, Cat. No. 79306) and the RNeasy® Mini Kit (Qiagen, Cat. No. 74106) following the manufacturer’s protocol. RNA concentration and purity were assessed using a NanoDrop ND-1000 spectrophotometer (NanoDrop Technologies, USA). Only samples with sufficient quality were used for downstream applications. Individual libraries were generated using Illumina TruSeq™ RNA Sample Preparation Kit (Illumina, San Diego, CA, USA).
description
not collected
geographic location (country and/or sea)
not applicable
isolate
Sus scrofa
organism
Sus scrofa
organism
kidney
organism part
ET Type
phenotype
Sus scrofa
scientific_name
male
sex
0309-Kidney
title