2025-09-12
ENA first public
ERC000011
ENA-CHECKLIST
Hebrew University, biochemistry department
INSDC center name
public
INSDC status
ERS25303368
SRA accession
E-MTAB-15383:HCNg_rep2
Submitter Id
ArrayExpress
broker name
HCN-2
cell line
Cortical neuron
cell type
not collected
collection date
human
common name
Protocols: Supraspliceosomes were prepared, as previously described {https://www.sciencedirect.com/science/article/pii/S1097276504004459?via%3Dihub }, from nuclear supernatants enriched in supraspliceosomes from the following cell- lines: HCN, U87MG and SH-SY5Y. Briefly, nuclear supernatants were prepared from purified cell nuclei by microsonication of the nuclei and precipitation of the chromatin in the presence of excess of tRNAs. All isolation steps were conducted at 4°C. The nuclear supernatant was fractionated on 10-45% (vol/vol) glycerol gradients. Centrifugations were carried out at 4°C in an SW41 rotor run at 41 krpm for 90 min [or an equivalent 2t = 2500 ( is in krpm; t is in hr)]. The gradients were calibrated using the tobacco mosaic virus as a 200S sedimentation marker. Supraspliceosome peak fractions were confirmed by Western Blot (WB) and by electron microscopy visualization. RNA extraction NEBNext® Multiplex Small RNA Library Prep Set for Illumina® (Set 1)
description
normal
disease
not collected
geographic location (country and/or sea)
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
brain
organism part
Homo sapiens
scientific_name
HCNg_rep2
title