2025-09-13
ENA first public
ERC000011
ENA-CHECKLIST
School of Biochemistry and Immunology, Trinity Biomedical Sciences Institute, Trinity College, Dublin 2, Ireland.
INSDC center name
public
INSDC status
ERS26815409
SRA accession
E-MTAB-15591:5019C_LPS_1h_S12
Submitter Id
ArrayExpress
broker name
primary cell line
cell line
bone marrow macrophage
cell type
not collected
collection date
house mouse
common name
Protocols: BMDMs were plated at 0.5 x 106 cells/mL (2 mL total volume) in a 6-well dish and left to adhere overnight at 37oC in a de-humidified incubator (21% O2, 5% CO2) before being treated with LPS (100 ng/mL) or left untreated. RNA isolation was carried using RNeasy® Plus kit (74136, Qiagen) following manufacturer’s suggestions and eluted RNA was purified using RNA Clean & Concentrator Kits (Zymo Research). RNA-seq samples libraries were prepared by Cambridge Genomic Services (CGS) using TruSeq Stranded mRNA (Illumina).
description
adult
developmental stage
Mitochondrial oxidative phosphorylation disorder due to a point mutation of mitochondrial DNA
disease
Mutant
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Mus musculus
organism
Mus musculus
organism
bone marrow
organism part
Mus musculus
scientific_name
LPS
stimulus
5019C_LPS_1h_S12
title