2025-09-13
ENA first public
ERC000011
ENA-CHECKLIST
Brunel University of London, College of Health, Medicine and Life Sciences, Division of Biosciences
INSDC center name
public
INSDC status
ERS26728278
SRA accession
E-MTAB-15526:K562-t(7;12) OSI930 1
Submitter Id
ArrayExpress
broker name
K562
cell line
erythroleukemia cell
cell type
not collected
collection date
human
common name
Protocols: K562-t(7;12) cells suspensions treated with each compounds were centrifuged at 400 x g for 5 minutes to pellet for subsequent extraction. Total RNA was extracted using Monarch Total RNA Miniprep Kit (NEB). Library construction was performed by Novogene UK (Cambridge, UK). A minimum of 400 ng (20 ng/μl) of high-quality total RNA was supplied for sequencing. Novogene UK constructed cDNA libraries by purification of messenger RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers followed by the second strand cDNA synthesis. The library was ready after end repair, A-tailing, adapter ligation, size selection, PCR amplification, and purification.
description
adult
developmental stage
leukemia
disease
t(7;12)(q36;1p13)
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
hematopoietic system
organism part
Homo sapiens
scientific_name
K562-t(7;12) OSI930 1
title