2025-09-13
ENA first public
ERC000011
ENA-CHECKLIST
SAMEA113577207
External Id
Department of Artificial Intelligence Medicine, Chiba University Graduate School of Medicine
INSDC center name
2023-06-07T10:51:32Z
INSDC last update
public
INSDC status
ERS15572121
SRA accession
E-MTAB-13029:Day2_Control
Submitter Id
ArrayExpress
broker name
CMO308
cell multiplexing oligo (cmo)
mouse embryonic stem cell
cell_type
missing: third party data
collection date
house mouse
common name
Protocols: Nanog-GFP MEFs were reprogrammed using the piggyBac MKOS reprogramming system with overexpression of genes of interest (BFP as a control, or Hic2) described earlier. Cells were collected on days 2, 4, 6 and 9, alongside Nanog-GFP MEFs and Nanog-GFP ESCs. Cells were labelled with a unique Cell Multiplexing Oligo (CMO) provided in the 10x Genomics 3’ CellPlex Kit according to the manufacturer’s instructions. Subsequently, cells were sorted with the FACS AriaII (BD Biosciences) for either mOrange (reporting for MKOS expression), GFP (reporting for NANOG expression, or both mOrange and BFP. After confirming the viability of cells after sorting using trypan blue and a haemocytometer, 10000 single cells per every 6 samples were pooled together, generating 2 pools that were each processed through the Chromium Single Cell Platform using a Chromium Next GEM Single Cell 3′ GEM Library and Gel Bead kit (v.3.1 chemistry, 10x Genomics) and a Chromium Next GEM Chip G kit following the manufacturer’s instructions. After confirming the viability of cells after sorting using trypan blue and a haemocytometer, 10000 single cells per every 6 samples were pooled together, generating 2 pools that were each processed through the Chromium Single Cell Platform using a Chromium Next GEM Single Cell 3′ GEM Library and Gel Bead kit (v.3.1 chemistry, 10x Genomics) and a Chromium Next GEM Chip G kit following the manufacturer’s instructions.
description
mouse embryo stage
developmental stage
normal
disease
BFP overexpression
genotype
missing: data agreement established pre-2023
geographic location (country and/or sea)
not applicable
isolate
951
number of cells detected
Mus musculus
organism
Mus musculus
organism
embryo
organism part
mouse embryonic fibroblast cell
progenitor cell type
Mus musculus
scientific_name
Day2_Control
title