2025-09-13
ENA first public
ERC000011
ENA-CHECKLIST
School of Biochemistry and Immunology, Trinity Biomedical Sciences Institute, Trinity College, Dublin 2, Ireland.
INSDC center name
public
INSDC status
ERS26815414
SRA accession
E-MTAB-15591:WT2_NS_S2
Submitter Id
ArrayExpress
broker name
primary cell line
cell line
bone marrow macrophage
cell type
not collected
collection date
house mouse
common name
Protocols: BMDMs were plated at 0.5 x 106 cells/mL (2 mL total volume) in a 6-well dish and left to adhere overnight at 37oC in a de-humidified incubator (21% O2, 5% CO2) before being treated with LPS (100 ng/mL) or left untreated. RNA isolation was carried using RNeasy® Plus kit (74136, Qiagen) following manufacturer’s suggestions and eluted RNA was purified using RNA Clean & Concentrator Kits (Zymo Research). RNA-seq samples libraries were prepared by Cambridge Genomic Services (CGS) using TruSeq Stranded mRNA (Illumina).
description
adult
developmental stage
normal
disease
wild type genotype
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Mus musculus
organism
Mus musculus
organism
bone marrow
organism part
Mus musculus
scientific_name
none
stimulus
WT2_NS_S2
title