2025-09-14
ENA first public
ERC000011
ENA-CHECKLIST
SAMEA114385211
External Id
The University of Manchester, UK
INSDC center name
2023-09-14T16:09:26Z
INSDC last update
public
INSDC status
ERS16374300
SRA accession
E-MTAB-13350:Sample 3
Submitter Id
ArrayExpress
broker name
not collected
collection date
human
common name
Protocols: Human hair follicle samples were obtained from consenting patients from the Crown Clinic, Manchester and were handled and stores in line with HTA regulations and ethical approval. Human hair follicles were cultured in an ex vivo organ culture model as described previously (Purba et al., 2019). Human hair follicles were subject to treatment with UK-5099 (10 and 40 µM Tocris #4186) for 48 h before isolation, embedding in frozen medium and freezing in liquid nitrogen or storage in RNA later. Total RNA was extracted using RNeasy Micro Kit (Qiagen #74004), according to the manufacturer’s protocol. Total RNA was submitted to the University of Manchester Genomic Technologies Core Facility (GTCF). Quality and integrity of the RNA samples were assessed using a 2200 TapeStation (Agilent Technologies). Illumina TruSeq Stranded mRNA kit, according to the manufacturer’s protocol.
description
adult
developmental stage
not collected
geographic location (country and/or sea)
1
individual
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
hair follicle
organism part
Homo sapiens
scientific_name
male
sex
Sample 3
title