2025-09-15
ENA first public
ERC000011
ENA-CHECKLIST
Zhongkai University of Agriculture and Engineering, Guangzhou, Guangdong Province, China.
INSDC center name
public
INSDC status
ERS26584172
SRA accession
E-MTAB-15515:ADH-72h-MG-3
Submitter Id
0.5
year
age
ArrayExpress
broker name
not collected
collection date
hybrid giant grouper
common name
Protocols: Before the start of the experiment, 600 healthy hybrid groupers with an average body weight of 42.3 ± 2.8 g were acclimatized in a recirculating aquaculture system (water temperature: 28–30°C; pH: 7.8 ± 0.2) and fasted for 24 h. The fish were then randomly allocated to two treatment groups: Control group ( con)which received no treatment; E. ictaluri-infected group (ADH) which was administered E. ictaluri via oral gavage. Total RNA was extracted from the midgut of  hybrid groupers by using the TRlzol Reagent and following the manufacturer’s instructions (Life Technologies, California, USA). cDNA libraries were constructed as per the instruction manual of NEBNext Ultra RNA Library Prep Kit.
description
fine
developmental stage
wilf type genotype
genotype
not collected
geographic location (country and/or sea)
same
individual
not applicable
isolate
Epinephelus fuscoguttatus x Epinephelus lanceolatus
organism
Epinephelus fuscoguttatus x Epinephelus lanceolatus
organism
gut
organism part
Epinephelus fuscoguttatus x Epinephelus lanceolatus
scientific_name
male
sex
Edwardsiella ictaluri
stimulus
Epinephelus fuscoguttatus × Epinephelus lanceolatus
strain
ADH-72h-MG-3
title