2025-09-15
ENA first public
ERC000011
ENA-CHECKLIST
Institut de Pharmacologie et de Biologie Structurale (IPBS), Université de Toulouse, CNRS, UPS
INSDC center name
public
INSDC status
ERS26806699
SRA accession
E-MTAB-15580:SubI_KO_2
Submitter Id
ArrayExpress
broker name
not collected
collection date
Protocols: WT and ΔsubI H37Rv were grown in triplicates in 7H9 medium supplemented with 10% ADC, 0,05% Tyloxapol, 1mM MgSO4, and 1mM cysteine until OD600 =0.8.   Total RNA was extracted from 10 mL of bacterial cultures grown to logarithmic phase (OD600nm = 0.8) using RNeasy kit (Qiagen).  Total Bulk RNA sequencing was performed Each extracted total RNA sample was barcoded with CEL-seq primers during the enzymatic lysis. It was then followed by fragmentation, end repair, poly(A)-tailing, reverse transcription, and second-strand synthesis. After second-strand synthesis, the barcoded samples were pooled into one library and amplified using in vitro transcription. Further, ribosomal aRNA was depleted. After depletion, adapter ligation, reverse transcription, and indexing PCR were performed to prepare a cDNA library.
description
subI KO
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Mycobacterium tuberculosis H37Rv
organism
Mycobacterium tuberculosis H37Rv
organism
Mycobacterium tuberculosis H37Rv
scientific_name
SubI_KO_2
title