2025-09-15
ENA first public
ERC000011
ENA-CHECKLIST
EMBL
INSDC center name
public
INSDC status
ERS25351001
SRA accession
ena-upload-cli
SUBMISSION_TOOL
0.9.0
SUBMISSION_TOOL_VERSION
51ebfea1-a455-4012-807b-9278d2c6bcaa
Submitter Id
4-20 hrs
age
not provided
collection date
fruit fly
common name
Wildtype Drosophila melanogaster flies were grown in population cages at 25°C. MiMIC fly lines with boundary insertions were grown in large-sized cages at 25°C. 0-16 hour embryo collections were aged at 25°C up to the desired time point (4-20 hours). Two independent collections were performed for each timepoint. The collected embryos were dechorionated using 50% bleach and formaldehyde fixed in 10 ml cross-linking solution (50 mM Hepes, 1 mM EDTA, 0.5 mM EGTA, 100 mM NaCl, 1.8 % formaldehyde, pH 8.0) and 30 ml n-heptane on a shaker table at RT for 15 minutes. The reaction was terminated with 125 mM glycine, 0.1% Triton X-100 in PBS. After washing out the fix, the embryos were blotted dry and frozen in liquid nitrogen.
description
insertion
genetic modification
y[1] w[*]; Mi{y[+mDint2]=B16_OR1-DsRed}MI14731/Cyo,GFP
genotype
not applicable
geographic location (country and/or sea)
egg laying
initial time point
Drosophila melanogaster
organism
Drosophila melanogaster
scientific_name
mixed sex
sex
BDSC 60957
strain
TADB_B16_OR1_GFP_17.1
title