2025-09-16
ENA first public
ERC000011
ENA-CHECKLIST
Gyeongsang National University Animal Genomics Laboratory
INSDC center name
public
INSDC status
ERS26815403
SRA accession
E-MTAB-15590:Oral6T_post_4m
Submitter Id
13
year
age
ArrayExpress
broker name
not collected
collection date
dog
common name
Protocols: Tumor tissue was collected by Tru-cut biopsy and preserved in RNAlater™ at three time points: before RT (Pre-RT), immediately after the final radiation fraction (Post-RT 0 m), and 4 months post-RT (Post-RT 4 m). Peripheral blood was drawn from the external jugular vein into Tempus™ Blood RNA Tubes at two time points: before RT (Pre-RT) and immediately after the final radiation fraction (Post-RT 0m). All samples were stored at −80 °C until processing. Animal procedures were approved by IACUC (GNU-240826-D0172). Total RNA was extracted from tumor (Pre-RT, Post-RT 0 m, Post-RT 4 m) and blood (Pre-RT, Post-RT 0 m) by the service provider according to internal standard operating procedures. Libraries were prepared using TruSeq Stranded Total RNA Library Prep Gold Kit (Illumina, San Diego, CA, USA) for tumor samples and TruSeq Stranded Total RNA Globin Kit (Illumina, San Diego, CA, USA) for blood samples.
description
adult
developmental stage
Oral melanoma
disease
wild type genotype
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Canis lupus familiaris
organism
Canis lupus familiaris
organism
Tumor
organism part
Tumor tissue
sample type
Canis lupus familiaris
scientific_name
Neutered male
sex
mixed-breed
strain
Post-RT 4 months
time point
Oral6T_post_4m
title