2025-09-16
ENA first public
ERC000011
ENA-CHECKLIST
MRC Laboratory of Medical Sciences, London, UK Institute of Clinical Sciences, Faculty of Medicine, Imperial College London, London, UK
INSDC center name
public
INSDC status
ERS25211993
SRA accession
E-MTAB-14477_2:Jabba_ct_atac1
Submitter Id
1-2.5
hour
age
ArrayExpress
broker name
not collected
collection date
fruit fly
common name
Protocols: For ATAC-seq on Jabba controls and knockdowns, single Drosophila embryos were collected, staged (nuclear cycle 14), and dechorionated using 50% bleach. Individual embryos were transferred into chilled lysis buffer, manually homogenized under a dissecting microscope, and nuclei were pelleted by centrifugation.  Transposition was carried out using 2.5 µL Tn5 transposase in a 10 µL reaction volume at 37°C for 30 minutes. Transposed DNA was purified using a MinElute cleanup kit (Qiagen) and amplified by PCR with indexed primers. Final libraries were cleaned using SPRI bead purification (1.2× ratio) and quantified by Qubit prior to sequencing.
description
NC14
developmental stage
sfGFPZId; SP/CyO;SiGFP-GAF(C)
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Drosophila melanogaster
organism
Drosophila melanogaster
organism
whole embryo
organism part
Drosophila melanogaster
scientific_name
mixed
sex
sfGFPZId; SP/CyO;SiGFP-GAF(C)
strain
Jabba_ct_atac1
title