2025-09-18
ENA first public
ERC000013
ENA-CHECKLIST
IOW Leibniz-Institut fur Ostseeforschung Warnemuende
INSDC center name
public
INSDC status
ERS26774049
SRA accession
223197:11adab19-75e0-4267-bb26-20956617ffd7
Submitter Id
temperate marginal sea biome [ENVO:01000856]
broad-scale environmental context
GFBIO
broker name
2021-08-01
collection date
3
m
depth
Seagrass leave biofilm from station BV01-A replicate a
description
plant matter [ENVO:01001121]
environmental medium
host associated
environmental package
yes
environmental_sample
Germany
geographic location (country and/or sea)
54.44
DD
geographic location (latitude)
9.87
DD
geographic location (longitude)
Baltic Sea coastal samples
geographic location (region and locality)
host associated
host associated environmental package
mimarks-survey
investigation type
sea grass bed [ENVO:01000059]
local environmental context
Samples were thawed and homogenized. A subsample of 4-6 leaves was transferred into bead-beating tube. This was placed on ice and sonicated twice for 7 min and bead beaten for 30 s at 4 m/s. DNA was extracted with PowerSoil DNA isolation - DNeasy PowerSoil Pro Kit
nucleic acid extraction
biofilm metagenome
organism
FWD:CCAGCASCYGCGGTAATTCC REV:ACTTTCGTTCTTGATYRR
pcr primers
BaltVib
project name
Stored at -80 C
sample material processing
Seagrass leave biofilm from station BV01-A replicate a
sample_description
biofilm metagenome
scientific_name
Illumina NovaSeq 6000
sequencing method
true
submitted to insdc
V4
target subfragment
EN82
title