2025-09-18
ENA first public
ERC000021
ENA-CHECKLIST
IOW Leibniz-Institut fur Ostseeforschung Warnemuende
INSDC center name
public
INSDC status
ERS26774016
SRA accession
223388:11adab19-75e0-4267-bb26-20956617ffd7
Submitter Id
temperate marginal sea biome [ENVO:01000856]
broad-scale environmental context
GFBIO
broker name
2021-07-25
collection date
0-0.01
m
depth
Coastal sediment metagenome taken in a Zostera marina meadow
description
not collected
m
elevation
shallow marine sediment [ENVO:03000034]
environmental medium
sediment
environmental package
yes
environmental_sample
Germany
geographic location (country and/or sea)
54.69
DD
geographic location (latitude)
9.97
DD
geographic location (longitude)
Baltic Sea coastal samples
geographic location (region and locality)
mimarks-survey
investigation type
microbial community [PCO:1000004]
local environmental context
Samples were thawed and homogenized. A subsample of 500 mg of sediment was transferred into bead-beating tube. This was placed on ice and sonicated twice for 7 min and bead beaten for 30 s at 4 m/s. DNA was extracted with PowerSoil DNA isolation - DNeasy PowerSoil Pro Kit
nucleic acid extraction
marine sediment metagenome
organism
FWD:CCAGCASCYGCGGTAATTCC REV:ACTTTCGTTCTTGATYRR
pcr primers
BaltVib
project name
Stored at -80 C
sample material processing
Coastal sediment metagenome taken in a Zostera marina meadow
sample_description
marine sediment metagenome
scientific_name
sediment
sediment environmental package
Illumina NovaSeq 6000
sequencing method
true
submitted to insdc
V4
target subfragment
20s
title