2025-09-29
ENA first public
ERC000011
ENA-CHECKLIST
University of Birmingham
INSDC center name
public
INSDC status
ERS26816858
SRA accession
E-MTAB-15572:Bulk RNA seq MK3
Submitter Id
ArrayExpress
broker name
megakaryocyte
cell type
not collected
collection date
house mouse
common name
Protocols: Mice were exsanguinated under terminal anesthesia by isoflurane/O2 (5%) gas. Blood was drawn from the inferior vena cava, using a 25-gauge needle, into 1:10 (volume-to-volume ratio) acid citrate dextrose anticoagulant.  Platelets and megakaryocytes were isolated and RNA was extracted using the RNeasy mini kit (Qiagen) according to manufacturer’s instructions Illumina/NEB kit protocol - Libraries were prepared using the NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina with NEBNext® rRNA Depletion Kit v2 (Human/Mouse/Rat) according to the manufacturer’s protocol. During this process, the libraires were indexed using NEBNext® Multiplex Oligos for Illumina® (96 Unique Dual Index Primer Pairs) Set 4. The prepared libraries were quantified via a fluorometric method involving a Promega QuantiFluor dsDNA assay and qualified using electrophoretic separation on the Agilent TapeStation 4200.
description
blood platelet disease
disease
germline genotype
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Mus musculus
organism
Mus musculus
organism
Mus musculus
scientific_name
C57BL/6J
strain
Bulk RNA seq MK3
title