characteristics.text: 2025-09-30
characteristics.characteristic: ENA first public
characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: UK Dementia Research Institute, King’s College London, London, UK
characteristics.characteristic: INSDC center name
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS24110477
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-15077:ZAP_S_KO_WT_1
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: HEK293T
characteristics.characteristic: cell line
characteristics.text: not collected
characteristics.characteristic: collection date
characteristics.text: human
characteristics.characteristic: common name
characteristics.text: Protocols: Cells were infected at MOI=3 and media was replaced 18 h post-inoculation with DMEM supplemented with 2% fetal bovine serum. At 48hpi, cells were washed with ice-cold PBS, irradiated once with 150 mJ/cm2 in a Stratalinker 2400 at 254 nm, harvested by scrapping in PBS, pelleted by centrifugation then resuspended in lysis buffer (50 mM Tris-HCl, pH 7.4; 100 mM NaCl; 1% Igepal CA-630; 0.1% SDS; 0.5% sodium deoxycholate; protease inhibitor). The nitrocellulose membrane strips were digested with 10μl proteinase K in 200μl proteinase K SDS buffer (10mM Tris-HCl pH 7.4, 100mM NaCl, 1mM EDTA, 0.2% SDS) for 1 hour at 50oC. The supernatant was transferred to a phase lock gel heavy tube. The RNA extracted were then phenol/chloroform extracted from the supernatant by adding 200μl of Phenol:Chloroform:Isoamyl Alcohol 25:24:1, incubating shaking for 5 min at 30°C, and centrifuged to separate the aqueous and organic phases. The aqueous phase was further extracted with 0.8ml of chloroform, centrifuged, and the upper phase containing the RNA is transferred to a new tube, and then precipitated with 20μl 3M NaAc, 0.75μl glycoblue and 0.5ml EtOH overnight at −20°C. The RNA is pelleted for 30 minutes at 4°C at 13000G. Custom individual cross-linking and immunoprecipitation (iCLIP) library construction protocol.
characteristics.characteristic: description
characteristics.text: ZAP_S_KO
characteristics.characteristic: genotype
characteristics.text: not collected
characteristics.characteristic: geographic location (country and/or sea)
characteristics.text: WT HIV-1
characteristics.characteristic: infect
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: ZAP_S_KO_WT_1
characteristics.characteristic: title