characteristics.text: 2025-09-30
characteristics.characteristic: ENA first public
characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: UK Dementia Research Institute, King’s College London, London, UK
characteristics.characteristic: INSDC center name
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS24235648
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-15095:XRN1_WT_2
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: HeLa
characteristics.characteristic: cell line
characteristics.text: not collected
characteristics.characteristic: collection date
characteristics.text: human
characteristics.characteristic: common name
characteristics.text: Protocols: Cells were infected at MOI=3. The medium was replaced 18 hours (h) post inoculation. Cell lysates and supernatants were collected at 48 hours post-infection. Supernatants were spun down at 300 ×g for five minutes and analysed by TZM-bl assay and immunoblotting. Total RNA was extracted using the Direct-zol RNA Miniprep Plus Kit The total RNA was purified with the Monarch Spin RNA Cleanup Kit (NEB). The 5’ RACE was performed from 500 ng purified RNA using the SMARTer RACE 5’/3’ Kit and according to the manufacturer’s instructions. 5’ RACE sequencing librairies were prepared using the Native Barcoding Kit 24 V14 (Oxford Nanopore Technologies).
characteristics.characteristic: description
characteristics.text: XRN1_WT
characteristics.characteristic: genotype
characteristics.text: not collected
characteristics.characteristic: geographic location (country and/or sea)
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: XRN1_WT_2
characteristics.characteristic: title