2025-10-02
ENA first public
ERC000011
ENA-CHECKLIST
ETH Zurich, Department of Biosystems Science and Engineering
INSDC center name
public
INSDC status
ERS26910117
SRA accession
E-MTAB-15622:Sample 1_sublib2
Submitter Id
ArrayExpress
broker name
Mixed H1, H9, Wibj2 and WTC
cell line
not collected
collection date
human
common name
Protocols: After d21 in culture 3 organoids per condition per cell line were dissociated individually using CyBio FeliX liquid handler robot with thermoshaker. For dissociation we used a papain-based dissociation kit (Miltenyi Neural dissociation kit). Each organoid was dissociated using 410uL of enzyme mix 1 and 6uL of enzyme mix 2. Then single cell suspension from the same condition were pooled together (H1 and H9; WTC and WIBJ2).  Each individual single cell suspension has been followed by  fixation and  permeabilization procedures, which were performed according to the manufacturer specification (ParseBiosciences, cell fixation kit v3, CF100).  Collected samples were processed for highly multiplexed single-cell RNA sequencing (scRNA-seq) using a split-pool combinatorial barcoding kit (ParseBiosciences, WT Mega kit v3, MG100).
description
not collected
geographic location (country and/or sea)
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
Homo sapiens
scientific_name
21
day
time
Sample 1_sublib2
title