2025-10-02
ENA first public
ERC000011
ENA-CHECKLIST
Institute of Life Sciences
INSDC center name
public
INSDC status
ERS23011091
SRA accession
E-MTAB-14795:NEK9_KO_rep2
Submitter Id
ArrayExpress
broker name
DU145
cell_line
prostate gland cancer cell
cell_type
not collected
collection date
human
common name
Protocols: DU145 NEK9 WT and KO cells were cultured in cell culture conditions in DMEM (10%FBS) . Cells were trypsinized and subjected to RNA isolation. Total RNA was isolated using Direct-Zol RNA miniprep (R2052) RNA isolation kit. To prepare an RNA-Sequencing library, 500ng of total RNA was used to isolate mRNA using magnetic beads (PolyA mRNA Isolation Module, NEB Cat No# E7490L). The RNA-Seq library was then prepared using an mRNA library preparation kit (NEB Cat No# E7770L) strictly following the vendor’s recommended protocol. Following library preparation, the library concentration was measured using Qubit 2.0 (Invitrogen), and the appropriate fragmentation sizes were confirmed using Bio-analyzer (Agilent Cat No# 5067-5582).
description
cancer
disease
NEK9 Knock out
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
prostate gland
organism part
Homo sapiens
scientific_name
NEK9_KO_rep2
title