2025-10-03
ENA first public
ERC000011
ENA-CHECKLIST
ICL-CFB
INSDC center name
public
INSDC status
ERS24147022
SRA accession
E-MTAB-15094:NM-2
Submitter Id
ArrayExpress
broker name
epicardial mesothelial cells (EMCs)
cell line
mesothelial cell
cell type
not collected
collection date
Norway rat
common name
Protocols: Cell lines from the culture were collected after epicardial conditioned media collection.  Total RNA was extracted from stressed EMCs using QIAzol Lysis Reagent (Qiagen) followed by purification with the Reasy Mini Kit (Qiagen) according to the manufacturer’s instructions. RNA concentration and purity were assessed using a Nanodrop 2000 spectrophotometer (Thermo Scientific), with samples exhibiting A260/A280 ratios between 1.8 and 2.1 and A260/A230 ratios > 2.0 considered suitable for downstream analysis. RNA integrity was further verified using an Agilent 5400 Fragment Analyzer, with RNA integrity numbers (RIN) > 8.0 required for library preparation.  RNA samples were sent to Novogene (UK) for library construction, quality control and sequencing.
description
adult
developmental stage
wild type genotype
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Rattus norvegicus
organism
Rattus norvegicus
organism
heart component
organism part
Rattus norvegicus
scientific_name
normoxia
stimulus
NM-2
title