2025-10-05
ENA first public
ERC000011
ENA-CHECKLIST
Genomix4Life s.r.l.
INSDC center name
public
INSDC status
ERS24558117
SRA accession
E-MTAB-15153:CTRL1
Submitter Id
ArrayExpress
broker name
mononuclear cell
cell type
not collected
collection date
human
common name
Protocols: A miRNA analysis on peripheral blood mononuclear cells (PBMCs) was performed by RNA-seq in 30 subjects, including 15 HD patients and 15 healthy controls. The subjects were recruited at the Oasi Research Institute - IRCCS (Troina, Italy).  PBMCs separation was performed using Ficoll-Paque (Ficoll Plaque PLUS–GE Healthcare Life Sciences, Piscataway, NJ, USA.Total RNA extraction from PBMC was performed using TRIzol reagent (TRIzol Reagent, Invitrogen Life Technologies, Carlsbad, CA, USA), according to the manufacturer’s instructions. RNA was stored at −80 ◦C until further processing.RNA concentration and purity were evaluated using NanoDrop™ 2000/2000c (Thermo Fisher Scientific),whereas sample integrity was analyzed by TapeStation 4200 (Agilent Technologies) using RNA ScreenTape Assay. 2.7. Indexed libraries were prepared from 250 ng purified RNA using QIAseq miRNA Library Kit (Qiagen) according to the manufacturer’s instructions. Libraries were quantified using the TapeStation 4200 (Agilent Technologies) and Qubit fluorometer (Invitrogen Co.), then pooled such that each index-tagged sample was present in equimolar amounts.
description
normal
disease
not collected
geographic location (country and/or sea)
1
individual
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
blood
organism part
Homo sapiens
scientific_name
CTRL1
title