2025-10-08
ENA first public
ERC000011
ENA-CHECKLIST
N/A
INSDC center name
public
INSDC status
ERS22993297
SRA accession
E-MTAB-14788:LUP1-2
Submitter Id
ArrayExpress
broker name
NHCF-V
cell_line
aCF+CKs
cell_type
not collected
collection date
human
common name
Protocols: Human adult cardiac fibroblasts (aCFs) were acquired from a commercial supplier; aCF+CKs were produced by addition of TNF-α and IL-4 into the culture medium; aVCFs were isolated from aCF+CKs by MACS against CD90 and VCAM1. Human cardiac fibroblasts were cultured in HFDM-1(+) medium supplemented with 1% (v/v) Newborn Calf Serum (NBCS). After initial expansion, aCFs were cultured with TNF-α and IL-4 in the medium. aCF-CKs were incubated with a biotin-conjugated anti-CD106 (VCAM1) or anti-CD90 (THY1) antibodies and anti-biotin microbeads for isolation of aVCFs by MACS. Total RNA was extracted using the RNeasy Plus Mini Kit following the manufacturer’s instructions (Qiagen). The library was constructed using the NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs) by GENEWIZ.
description
adult
developmental stage
normal
disease
wild type genotype
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
heart
organism part
Homo sapiens
scientific_name
LUP1-2
title