2025-10-09
ENA first public
ERC000047
ENA-CHECKLIST
EMG
INSDC center name
public
INSDC status
ERS26975514
SRA accession
SRR23278403_binner12_Refined_5
Submitter Id
Many fragments with little to no review of assembly other than reporting of standard assembly statistics
assembly quality
metaspades_v3.15.5
assembly software
default
binning parameters
MGnify-genomes-generation-pipeline_v1.0.0
binning software
human digestive system
broad-scale environmental context
EBI-EMG
broker name
2022-10-21
collection date
99.85
%
completeness score
CheckM2_v1.0.1
completeness software
0.08
%
contamination score
This sample represents a Third Party Annotation (TPA) Metagenome-assembled genome assembled from the metagenomic run SRR23278403 of study SRP420134.
description
oral sample
environmental medium
China
geographic location (country and/or sea)
missing: third party data
DD
geographic location (latitude)
missing: third party data
DD
geographic location (longitude)
metagenome-assembled genome
investigation type
human oral metagenome
isolation_source
oral cavity
local environmental context
human oral metagenome
metagenomic source
uncultured Capnocytophaga sp.
organism
Caries is one of the irreversible and long-term oral diseases characterized by the destruction of dental hard tissues. Altered oral microbiota has been proposed to mediate caries however, the precise mechanism of caries remains unclear, and caries-specific microbiota, metabolites, and their interactions remain poorly understood. To address this issue, we performed metagenomic (n = 62) and metabolomic (n = 32) profiling of plaque samples based on caries-affected patients and caries-free control cohorts. Metagenomic data showed obvious dysbiosis of microbiota in caries-active patients and Prevotella spp. showed significant correlated with caries activity and relative abundance of Streptococcus mutans in dental plaque. We further identified Prevotella spp. were also the key connectors in caries-active derived microbiota network. Moreover, dysregulated carbohydrate metabolism, especially starch catabolism associated function genes, was found to be correlated with both caries activity. Intriguingly, these genes were mainly expressed by Prevotella spp. Last but not least, we found supragingival microbiota and metabolites showing significant differences has great power to distinguish caries-active patients from healthy controls.</p><p>Collectively, our study revealed supragingival microbiota and metabolites alterations, highlighted the massive alterations of these, which empower to recognize caries-active patients suggested potential roles of microbiota dysbiosis in caries pathogenesis, and offered new clues to understand inter-species interaction in this process. Our study provided a valuable resource for future studies, and would facilitate facilitate potential clinical applications of featured microbiota and (or)metabolites to conduct precision medicine on caries-active population.
project name
SAMN32963687
sample derived from
uncultured Capnocytophaga sp.
scientific_name
Illumina NovaSeq 6000
sequencing method
multi-marker approach
taxonomic identity marker
Metagenome-assembled genome: SRR23278403_binner12_Refined_5
title