2025-10-10
ENA first public
ERC000011
ENA-CHECKLIST
SAMEA114479424
External Id
University Medical Center Hamburg-Eppendorf I. Medical Department
INSDC center name
2023-10-10T13:08:35Z
INSDC last update
public
INSDC status
ERS16461341
SRA accession
E-MTAB-13405:sample003_GEX
Submitter Id
ArrayExpress
broker name
mononuclear cell
cell_type
not collected
collection date
human
common name
Protocols: Peripheral blood mononuclear cells (PBMCs) were isolated from healthy individuals blood using density gradient centrifugation (Capricorn Scientific). Freshly isolated PBMCs were immediately used for experiments or cryopreserved for subsequent analysis in heat inactivated fetal bovine serum (FBS) (Capricorn Scientific) supplemented with 10% (v/v) dimethyl sulfoxide (DMSO) (Sigma-Aldrich) and stored in liquid nitrogen. Freshly isolated PBMCs from three healthy individuals were stained using surface markers antibodies and barcoded using antibodies for CITE-Seq using TotalSeq A antibodies. (BioLegend, USA) (Key Resources Table). CD8+ IL-7R+ HLA-DP-, IL-7R+ HLA-DP+, IL-7R- HLA-DP+ cells were FACS-sorted, using a FACSAria fusion (BD Biosciences). FACS-sorted cells were washed once with autoMACS Running Buffer (Miltenyi Biotec) and resuspended in PBS. 25.000 cells were used for GEM generation through the 10X Chromium Controller using the 10x V3 B Chip GEM generation kit (10x Genomics, USA).
description
adult
developmental stage
normal
disease
not collected
geographic location (country and/or sea)
CD8+ IL7-HLA+
immunophenotype
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
blood
organism part
GEX
protocol
Homo sapiens
scientific_name
sample003_GEX
title