2025-10-10
ENA first public
ERC000011
ENA-CHECKLIST
Department of Developmental Biology and Genetics, Indian Institute of Science, Bangalore and Department of Life Sciences, Imperial College London, UK
INSDC center name
public
INSDC status
ERS21355071
SRA accession
E-MTAB-14556:WB3
Submitter Id
ArrayExpress
broker name
intestinal epithelial cell
cell_type
not collected
collection date
house mouse
common name
Protocols: Small intestine was isolated from mice for organoid preparation. Preparation was as per protocols published by StemCell Organoids prepared from wild type and S839I mice were cultured for 3 days after passaging and then treated with interferon gamma or uroguanylin or both together for 16h. Total RNA purified form cultured organoids using Invitrogen RNA purification kits. RNA was treated with DNAse on the column. mRNA fragment and synthesis of cDNA followed by end repair and dA tailing. cDNA purified and amplified by PCR
description
wild type genotype
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Mus musculus
organism
Mus musculus
organism
epithelium of small intestine
organism part
Mus musculus
scientific_name
C57BL
strain
WB3
title
interferon gamma
nanogram per milliliter
treatment