2025-10-11
ENA first public
ERC000011
ENA-CHECKLIST
Viral Genetics and Biosafety Unit, French Agency for Food, Environmental and Occupational Health and Safety (ANSES), Ploufragan-Plouzané-Niort Laboratory
INSDC center name
public
INSDC status
ERS24422635
SRA accession
E-MTAB-15113:ST-1-24-Pu
Submitter Id
Not applicable
week
age
ArrayExpress
broker name
Swine Testis cells (ST)
cell line
Testicular cells
cell type
not collected
collection date
pig
common name
Protocols: ST cells were cultivated in EMEM (Lonza), 10% FBS, 1% Penicilinn/Streptomycin and 1% Glutamax (ThermoFisher). After two washes with EMEM but without FBS, ST cells (after 1 day of plating) were inoculated with TGEV at multiplicity of infection of 0.06. The cells were washed twice after 1 hour of inoculation and EMEM,1% Penicilinn/Streptomycin and 1% Glutamax was added. The corresponding controls were mock-infected with ST cells supernatant. The total RNA of jejunum crypts, 3D organoids, 2D organoids, piglet jejunums and ST cells were extracted with Trizol Reagent, according to the supplier’s recommendations. After Turbo DNAse treatment and validation of RNA quality, 500ng of RNA were used to build the sequencing librairies. Libraries were generated with the Illumina Stranded mRNA Kit, according to the supplier’s recommendations. Essentially, the mRNA were first enriched by the capture of polyA tails via oligoDT. After enzymatic fragmentation, the cDNA were synthetized and the protruding ends of the cDNA were converted into blunt ends by exonuclease/polymerase activities. The 3' ends were adenilated and the adaptators were ligated. The libraries were amplified by PCR reactions and sequenced.
description
Not applicable
developmental stage
TGEV infected
disease
Not applicable
genotype
not collected
geographic location (country and/or sea)
Not applicable
individual
TGEV infected
infect
MOI 0.06
infection dose
not applicable
isolate
Sus scrofa
organism
Sus scrofa
organism
Not applicable
organism part
Replicate 1/3
replicate
TGEV infected ST cells (MOI 0.06 ; 24hpi ; replicate 1)
sample title
Sus scrofa
scientific_name
Male
sex
Not available
strain
ST-1-24-Pu
title