2025-10-11
ENA first public
ERC000011
ENA-CHECKLIST
Viral Genetics and Biosafety Unit, French Agency for Food, Environmental and Occupational Health and Safety (ANSES), Ploufragan-Plouzané-Niort Laboratory
INSDC center name
public
INSDC status
ERS24422601
SRA accession
E-MTAB-15113:O-20-cry
Submitter Id
0
week
age
ArrayExpress
broker name
Not applicable
cell line
Not applicable
cell type
not collected
collection date
pig
common name
Protocols: Crypts were isolated from jejunum of stillborn piglets. This represents a control sample, and as such no treatment was applied. The total RNA of jejunum crypts, 3D organoids, 2D organoids, piglet jejunums and ST cells were extracted with Trizol Reagent, according to the supplier’s recommendations. After Turbo DNAse treatment and validation of RNA quality, 500ng of RNA were used to build the sequencing librairies. Libraries were generated with the Illumina Stranded mRNA Kit, according to the supplier’s recommendations. Essentially, the mRNA were first enriched by the capture of polyA tails via oligoDT. After enzymatic fragmentation, the cDNA were synthetized and the protruding ends of the cDNA were converted into blunt ends by exonuclease/polymerase activities. The 3' ends were adenilated and the adaptators were ligated. The libraries were amplified by PCR reactions and sequenced.
description
Piglet
developmental stage
Non-infected (control)
disease
Not applicable
genotype
not collected
geographic location (country and/or sea)
Pig donor 6
individual
Non-infected (control)
infect
Not applicable
infection dose
not applicable
isolate
Sus scrofa
organism
Sus scrofa
organism
Intestinal jejunum crypts
organism part
Replicate 3/3
replicate
Jejunum crypts of stillborn piglet (pig donor 6)
sample title
Sus scrofa
scientific_name
Not available
sex
SPF Large White
strain
O-20-cry
title