2025-10-30
ENA first public
ERC000024
ENA-CHECKLIST
National Oceanography Centre
INSDC center name
public
INSDC status
ERS22558521
SRA accession
ena-SAMPLE-TAB-09-12-2024-16:19:12:803-5671
Submitter Id
aquatic biome ENVO_00002030
broad-scale environmental context
2019-11-14T18:26:00
collection date
1
m
depth
RoCSI bihourly temporal sample (autonmously)
description
liquid water ENVO_00002006
environmental medium
time series design EFO_0001811
experimental factor
Atlantic Ocean
geographic location (country and/or sea)
50.891513
DD
geographic location (latitude)
-1.394115
DD
geographic location (longitude)
United Kingdom
geographic location (region and locality)
estuarine biome ENVO_01000020
local environmental context
Modified Qiagen AllPrep DNA/RNA Mini Kit
nucleic acid extraction
marine metagenome
organism
RoCSI_Soton_Temporal
project name
Robotic Cartridge Sampling Instrument (RoCSI)
sample collection device
Autonomous filtration through 0.22µm Sterivex and autonomous preservation with RLT+ buffer Qiagen. Removed from the RoCSI device and stored in -80°C freezer within 24 hours of collection.
sample collection method
National Oceanography Centre, Southampton, UK
sample storage location
-80
°C
sample storage temperature
500
mL
sample volume or weight for DNA extraction
marine metagenome
scientific_name
0.00022
mm
size-fraction lower threshold
16S rRNA|18S rRNA
target gene
16S V4-V5 |18S V9
target subfragment
10.5472467
ºC
temperature
RoCSI_49
title
2.40033333
m
total depth of water column