2025-10-30
ENA first public
ERC000011
ENA-CHECKLIST
Institute for Microbiology and Infection, University of Birmingham
INSDC center name
public
INSDC status
ERS24142615
SRA accession
E-MTAB-15087:RNAseq_minusNB_b
Submitter Id
ArrayExpress
broker name
not collected
collection date
Protocols: All cells were harvested by high speed centrifugation (approx 5000 xG). Pellets were flash froze in liquid nitrogen before storage at -80 oC. For each biological replicate, a single colony was inoculated into 10 ml M22+ media and grown at 30 degrees Celsius with vigorous shaking for 2-3 days. This 10 ml culture was used to start a larger culture, typically 100 - 200 ml. The larger culture had a starting OD600 of 0.05, and was grown throughout the day at 30 degrees Celsius with shaking until exponential phase, approx 0.5 OD600. At which point novobiocin was added for 20 minutes if required.  When stated, novobiocin was added to a exponentially growing cells (OD600 0.5) to a final concentation of 2 μg/ml Total RNA was isolated as described previously (1) with the exception that RNA concentration and quality was determined on an Agilent 2200 Tapestation.   1 - Forrest, D., James, K., Yuzenkova, Y. & Zenkin, N. Single-peptide DNA-dependent RNA polymerase homologous to multi-subunit RNA polymerase. Nat. Commun. 8, 15774 (2017) Library construction for strand-specific RNA-seq was performed Gene Wiz (Azenta)
description
not collected
geographic location (country and/or sea)
M22+ media. 30 degrees Celsius
growth condition
not applicable
isolate
Cereibacter sphaeroides
organism
Cereibacter sphaeroides
organism
biological replicate b
replicate
N/A
response to treatment
Cereibacter sphaeroides
scientific_name
Rhodobacter sphaeroides 2.4.1
strain
RNAseq_minusNB_b
title