2025-10-30
ENA first public
ERC000011
ENA-CHECKLIST
Laboratory of Human Immunology (Single Cell Genomics), WPI Immunology Frontier Research Center, The University of Osaka
INSDC center name
public
INSDC status
ERS27122152
SRA accession
E-MTAB-15929:Stromal cell 1
Submitter Id
ArrayExpress
broker name
CD31- CD45- Ter119- cells
cell type
not collected
collection date
house mouse
common name
Protocols: In accordance with the manufacturer’s recommendations, approximately 16,500 live cells per sample were loaded onto the Chromium controller to generate 10,000 single-cell gel-bead emulsions for library preparation and sequencing.  Sinlge cell droplets were generated by 10x Genomics Chromium Controller, and cDNAs with UMIs were generated at single-cell level. Raw FASTQ files obtained from stromal cell populations (Stromal cell 1 and Stromal cell 2; CD45-Ter119-CD31+ and RANKL+CD45-Ter119-CD31+, respectively) were processed using the Cell Ranger pipeline (v6.0.0 or v7.1.0, 10x Genomics) for alignment to the mm10 genome, UMI collapsing, and UMI counting for each gene–cell (barcode) combination.
description
not collected
geographic location (country and/or sea)
not applicable
isolate
Mus musculus
organism
Mus musculus
organism
bone marrow
organism part
Mus musculus
scientific_name
Stromal cell 1
title