2025-10-30
ENA first public
ERC000011
ENA-CHECKLIST
A*STAR Skin Research Labs
INSDC center name
public
INSDC status
ERS27074142
SRA accession
E-MTAB-15754:UnmodNTERT_UNT3
Submitter Id
ArrayExpress
broker name
N/TERT1
cell line
keratinocyte
cell type
not collected
collection date
human
common name
Protocols: TRIzol is added directly to the cells in a 6-well plate after overnight stimulation.  Immortalized N/TERT keratinocytes are seeded on a 6-well plate and treated at 80% confluency with 50ng/ml of IFN-gamma (or untreated) overnight. Total RNA extraction was performed using TRIzol reagent. Isopropyl alcohol is added to the the cells homogenized in TRIzol, followed by centrifugation. Library construction was conducted via standard protocol  with Macrogen (South Korea).
description
wild type genotype
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
skin epidermis
organism part
Homo sapiens
scientific_name
untreated
stimulus
UnmodNTERT_UNT3
title