2025-10-30
ENA first public
ERC000011
ENA-CHECKLIST
The Second Hospital & Clinical Medical School, Lanzhou University
INSDC center name
public
INSDC status
ERS26984918
SRA accession
E-MTAB-15692:Sample 6
Submitter Id
ArrayExpress
broker name
HUVEC cell line
cell line
Sensitive
cell type
not collected
collection date
human
common name
Protocols: Apatinib sensitive and resistant primary HUVECs were cultured in 2 μmol/L apatinib for 48 h, and collected for RNA isolation. Total RNA was extracted using the TRIzol reagent (Invitrogen, CA, USA) according to the manufacturer's protocol. RNA purity and quantification were evaluated using the NanoDrop 2000 spectrophotometer (Thermo Scientific, USA). RNA integrity was assessed using the Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA) Sequencing libraries were prepared using the NEBNext Ultra RNA Library Prep Kit for Illumina, and library quality was assessed using the Agilent 2100 Bioanalyzer System (Agilent Technologies).
description
adult
developmental stage
cancer
disease
wild type genotype
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
vasculature
organism part
Homo sapiens
scientific_name
Sample 6
title