2025-10-30
ENA first public
ERC000011
ENA-CHECKLIST
UniTo
INSDC center name
public
INSDC status
ERS26976748
SRA accession
E-MTAB-15684:CTCF_ctr_2
Submitter Id
ArrayExpress
broker name
WTC11
cell line
hiPSCs derived cardiomyocytes
cell type
not collected
collection date
human
common name
Protocols: hiPSCs derived cardiomyocytes were differentiated in a monolayer and collected at day 25 of differentiation. Cardiomyocites were directly lysed on the plate and collected. half of the samples (indicated with TET) were treated with tetracycline 1 ug/ml since the split of hiPSCs immediately before differentiation RNA was extracted with the ZymoResearch kit and converted to cDNA.  Zymo - Seq RiboFree Total RNA Library Prep Kit (R3000S) + the Zymo-See Index Primer Set (D3007). 300 ng were pooled with final concentration of ~6ng/uL. The average size of the library is ~350 bp
description
day 25
developmental stage
normal
disease
CTCF sh
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
cardiomyocytes
organism part
Homo sapiens
scientific_name
CTCF_ctr_2
title