2025-10-30
ENA first public
ERC000011
ENA-CHECKLIST
UniTo
INSDC center name
public
INSDC status
ERS27024496
SRA accession
E-MTAB-15711:D7 replicate 1 multiplexing seq 2
Submitter Id
ArrayExpress
broker name
WTC11
cell line
not collected
collection date
human
common name
Protocols: Digestion with versene and trippleE. Then cells were labeled with CMO, and sorted for vital cells and then loaded on the 10X controller. We processed the organoids with trypsin and obtained a single-cell suspension. We then labeled the cells with cell multiplexing oligos (CMO) before loading on a reaction of 3' Next GEM single cellv3.1 kit.  Cells were washed with PBS and 1% BSA (Miltenyi) and then labeled with CMOs following the 10x Genomics protocol (CG000391 RevA). After three washes with PBS and 1% BSA, cells were incubated for 15 minutes at room temperature with Fixable Viability Dye eFluor™ 780 (Invitrogen 1:1000 dilution). Cells were washed again and sorted with a SONY SH800S sorter with a 100 µm chip in FACS tubes, then pooled together in equal quantities, and resuspended at 1,600 cells/µL to be loaded on the 10X chromium with a target of 16,000 cells per reaction. 10X genomics technology with CG000388 Rev B (with cell multiplexing oligos). Cells were loaded on a chip G. the single cell library was created with CG000388 Rev B (with cell multiplexing oligos).
description
D7 cardioid
developmental stage
normal
disease
mixed
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
heart left ventricle cardioid
organism part
multiplexing
protocol
Homo sapiens
scientific_name
D7 replicate 1 multiplexing seq 2
title