2025-10-30
ENA first public
ERC000011
ENA-CHECKLIST
1) University of Arkansas 2) North Dakota State University
INSDC center name
public
INSDC status
ERS27132868
SRA accession
E-MTAB-15925:Fall Female Eye Tissue Jc144
Submitter Id
ArrayExpress
broker name
36.221194, -94.484556
collection coordinates (decimal degrees)
not collected
collection date
buckeye
common name
17-October-2021
date collected
Protocols: In 2019, 2020, and 2021 we caught wild butterflies in Summer (June/July) and Fall (late September/October) in Arkansas, USA grasslands. Butterflies were caught in the 90 minute period before and after solar noon and were decapitated within 5 minutes of capture. Heads were immediately placed in 1000 μL RNALater (Invitrogen) and placed on ice. We took collected heads back to the lab and stored them at 4° C overnight before transferring to -20° C.  From heads, we dissected out retinal tissue, which  included a thin coating of lamina that was impossible to remove without removing underlying eye tissue.  Dissection occurred immediately prior to RNA purification in RNALater Solution. Tissue was homogenized with mortar and pestle and purified with Nucleopsin minikits (Machery-Nagel). We sent purified RNA samples to Novogene for cDNA library prep (polyA enrichment).
description
adult
developmental stage
Gentry, AR, USA
field collection site
wild type genotype
genotype
not collected
geographic location (country and/or sea)
Jc144
individual
not applicable
isolate
Junonia coenia
organism
Junonia coenia
organism
retina
organism part
Junonia coenia
scientific_name
Fall
seasonal morph
female
sex
Fall Female Eye Tissue Jc144
title