2025-10-30
ENA first public
ERC000011
ENA-CHECKLIST
ZJU
INSDC center name
public
INSDC status
ERS27095647
SRA accession
E-MTAB-15770:Sample 1
Submitter Id
ArrayExpress
broker name
CD45+
cell type
not collected
collection date
house mouse
common name
Protocols: We sorted live, CD45+ cells from single-cell suspensions of tumors from 3 mice using a FACS (BD Biosciences).  The single-cell suspension was subjected to quality control with the following criteria: cell viability must meet the requirements (>80% by AO/PI fluorescent staining or >75% by trypan blue exclusion), cell concentration between 700-1,200 cells/μL, cell diameter ranging from 5 to 30 μm, total cell count up to 100,000, clean suspension background with no significant debris or impurities. After quality control, the viable cells were washed, resuspended, and prepared as a single-cell suspension at an appropriate concentration. The cell suspension was then loaded according to the target cell recovery count. Following loading, the formation of GEMs (Gel Bead-In-Emulsions) was monitored. If GEMs formed successfully, they were transferred to a PCR tube for subsequent reverse transcription and library construction. Once the constructed library passed quality control, it was subjected to sequencing.
description
adult
developmental stage
MG53 ko fl mice
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Mus musculus
organism
Mus musculus
organism
anatomy basic component
organism part
Mus musculus
scientific_name
Sample 1
title