2025-10-30
ENA first public
ERC000011
ENA-CHECKLIST
Institut Gustave-Roussy
INSDC center name
public
INSDC status
ERS26973405
SRA accession
E-MTAB-15673:G500025_HAP1_KMT2C1_S25
Submitter Id
ArrayExpress
broker name
HAP-1
cell line
neoplastic cell
cell type
not collected
collection date
human
common name
Protocols: HAP1 parental and KO cell lines (RRID:CVCL_Y019) for ARID1A, ARID1B, ARID2, PBRM1, SMARCA2, SMARCA4, KMT2C, KMT2D, CREBBP or SETD2 were purchased from Horizon Discovery. HAP-1 KO cell lines for SMARCB1, BAP-1 or EED were obtained from Drs. Raphaël Margueron (Institut Curie, UMR934, Paris) and Eric Pasmant’s teams (Institut Cochin, Paris).  HAP-1 cells were cultured in Iscove’s Modified Dulbecco’s Medium (IMDM, Gibco) supplemented with 10% FBS (Sigma), 1% Penicillin-Streptomycin (Gibco), 1% Sodium Pyruvate (Gibco), 1% Sodium Bicarbonate (Gibco) and 1% Non-Essential Amino Acids (Gibco).  70-80% confluent cells were harvested, and total RNA was extracted using Rneasy Mini Kit (Qiagen, 74104) with DNAse treatment, according to the manufacturer’s instructions. Every RNA sample was quantified with a Qubit Fluorometer and evaluated for quality controls using Agilent 2100 Bioanalyzer Instrument (RRID:SCR_018043). After RNA Integrity Number (RIN) quality control, cDNA libraries were generated using the NEBNext Ultra II RNA Library Prep Kit (NEB #E7775) on Bravo Automated Liquid Handling Platform (RRID:SCR_026137).
description
adult
developmental stage
chronic myeloid leukemia
disease
KMT2C_KO
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
blood
organism part
1
replicate
Homo sapiens
scientific_name
G500025_HAP1_KMT2C1_S25
title