2025-10-30
ENA first public
ERC000011
ENA-CHECKLIST
Ottawa Hospital Research Institute
INSDC center name
public
INSDC status
ERS27106398
SRA accession
E-MTAB-15806:Sample 1
Submitter Id
ArrayExpress
broker name
not collected
collection date
fruit fly
common name
Protocols: To eliminate/reduce yeast (and yeast RNA) in the intestine, flies were transferred to bottles containing filter paper saturated with 5% glucose in water for four hours. The flies were anesthetized by CO2 and the entire intestine excluding the crop was excised in PBS. Isolated guts were then placed into Eppendorf tubes. Total RNA was isolated from 30 female guts using Qiagen’s RNeasy Plus kit. RNA sample quality assessment was performed with the Fragment Analyzer Standard Sensitivity RNA assay (Agilent) and concentration measured with the Qubit 3.0 HS RNA assay (Thermo).   NGS libraries were prepared with the Stranded mRNA library prep kit (Illumina) using 400 ng of total RNA input.
description
not collected
geographic location (country and/or sea)
not applicable
isolate
Drosophila melanogaster
organism
Drosophila melanogaster
organism
gut
organism part
Drosophila melanogaster
scientific_name
female
sex
Sample 1
title