2025-10-30
ENA first public
ERC000011
ENA-CHECKLIST
Temasek Life Sciences Laboratory
INSDC center name
public
INSDC status
ERS27155964
SRA accession
E-MTAB-15892:Sample 12
Submitter Id
8
day
age
ArrayExpress
broker name
not collected
collection date
thale cress
common name
Protocols: Samples were harvested, washed once in sterile MilliQ water, and snap-frozen in liquid nitrogen Seeds were surface-sterilised and stratified for three days in the dark at 4C, then germinated on full-strength MS media (1% sucrose, 1% agar, pH5.8) for eight days at 22C under 24h light Seedlings were transferred from solid media to liquid half-strength MS Basal (pH5.7) media and incubated at 22°C for 48h under Frozen samples are milled in extraction buffer and total RNA extracted using the QIAGEN Plant RNeasy kit Frozen samples are milled in extraction buffer and total RNA extracted using the QIAGEN Plant RNeasy kit
description
seedling development stage
developmental stage
Col-0
ecotype
cat-1,5,8
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Arabidopsis thaliana
organism
Arabidopsis thaliana
organism
whole plant
organism part
3
replicate
Arabidopsis thaliana
scientific_name
Sample 12
title