2025-10-30
ENA first public
ERC000011
ENA-CHECKLIST
Department of Obstetrics and Gynecology, Daping Hospital, Army Medical University (Third Military Medical University)
INSDC center name
public
INSDC status
ERS26973351
SRA accession
E-MTAB-15670:B80203
Submitter Id
52
year
age
ArrayExpress
broker name
experimental cell
cell type
not collected
collection date
human
common name
Protocols: early endometrial adenocarcinoma of human (lesions not extending beyond the uterus, FIGO 2009 stage I and II, all patients underwent standard endometrial cancer surgery (total hysterectomy + bilateral adnexectomy + pelvic lymph node dissection ± abdominal main lymph node dissection) we added Hibernate-A buffer containing 10% fetal bovine serum to terminate the digestion and gently pipetted the tissues multiple times for dissociation. The dissociated cells were filtered through a 40 mm cell strainer to remove cell aggregates and ultimately obtain a single-cell suspension for encapsulation with 10x Genomics. We used Single-cell 30 Library and Gel Bead Kit V3 (10x Genomics) for cell capture and cDNA synthesis. The cells were counted using Count Star and resuspended. Cell suspensions were loaded on the 10x Genomics scRNA-seq platform to generate single-cell gel beads in the emulsion (GEM). The mRNA of single cells was reverse transcribed to produce cDNA, and the individual GEMs were simultaneously barcoded, which were programmed at 53°C for 45 min, 85°C for 5 min, and held at 4°C. The cDNA library was then generated, amplified, and assessed for quality using the Agilent 4200. Sequencing was performed.
description
adult
developmental stage
endometrial disease
disease
not collected
geographic location (country and/or sea)
non-recurrent
individual
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
endometrium
organism part
Homo sapiens
scientific_name
female
sex
B80203
title