2025-10-30
ENA first public
ERC000011
ENA-CHECKLIST
Wirka Lab, UNC Chapel Hill
INSDC center name
public
INSDC status
ERS27064905
SRA accession
E-MTAB-15722:TWIST1 monomer 3
Submitter Id
ArrayExpress
broker name
HCAMSC
cell line
smooth muscle cell
cell type
not collected
collection date
human
common name
Protocols: Human coronary artery smooth muscle cells (HCAMSCs) were ordered from Lonza. HCAMSCs were transduced with lentivirus and prepared for RNA isolation. HCAMSCs were cultured in Lonza's SmBM medium with SmGM-2 Smooth Muscle Cell Growth Medium-2 Bullet Kit, excluding gentamicin sulfate. HCAMSCs were used between passage 5-6 for all experiments.  Once HCAMSCs reached 70% confluence in 6-well plates, serum-free media was applied for 24 hours prior to lentiviral transduction with polybrene. After overnight incubation, the medium was replaced with fresh full-serum SmGM medium. HCASMCs were harvested for RNA isolaion 48 hours later.   FastQ files were processed using nf-core/rnaseq (version 3.17.0) pipeline. Pre-processing to infer strandedness was performed using fq (v0.12.0) and 203 Salmon (v1.10.3).  RNA extraction was performed using the Zymo Quick-RNA Microprep Kit.  Following quality control, the NEBNext Ultra II Directional Kit was used for library preparation.
description
adult
developmental stage
normal
disease
TWIST1 in pLJM1 backbone
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
coronary artery
organism part
Homo sapiens
scientific_name
TWIST1 monomer 3
title