2025-10-30
ENA first public
ERC000011
ENA-CHECKLIST
Laboratory of Mucosal Immunology, VIB Center for Inflammation Research, Ghent, Belgium
INSDC center name
public
INSDC status
ERS27126355
SRA accession
E-MTAB-15850:Sample 3
Submitter Id
15
day
age
ArrayExpress
broker name
dendritic cell
cell type
not collected
collection date
house mouse
common name
Protocols: Single cell suspensions of the lung were obtained and cells were stained for 30 minutes at 4 degrees celcius before sorting. Following sorting strategy was used: live cells, CD45 IV negative cells. For each sample, 7500 immune cells were sorted (CD45+) and 7500 structural cells (CD45-, CD31-, Ter119-, EPCAM+) Single live cells were sorted and loaded onto a Chromium GemCode Single Cell Instrument (10x Genomics) to generate single-cell gel beads in emulsion (GEMs) The scRNA-Seq libraries were prepared using the GemCode Single Cell 3’ Gel Bead and Library kit, version NextGEM 3.1 (10x Genomics) according to the manufacturer’s instructions
description
Alveolarisation phase lung
developmental stage
wild type genotype
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Mus musculus
organism
Mus musculus
organism
lung
organism part
Mus musculus
scientific_name
male
sex
PVM - PBS
stimulus
C57Bl6/J
strain
Sample 3
title