2025-10-30
ENA first public
ERC000011
ENA-CHECKLIST
Wirka Lab, UNC Chapel Hill
INSDC center name
public
INSDC status
ERS27064918
SRA accession
E-MTAB-15722:TWIST1-TWIST1 4
Submitter Id
ArrayExpress
broker name
HCAMSC
cell line
smooth muscle cell
cell type
not collected
collection date
human
common name
Protocols: Human coronary artery smooth muscle cells (HCAMSCs) were ordered from Lonza. HCAMSCs were transduced with lentivirus and prepared for RNA isolation. HCAMSCs were cultured in Lonza's SmBM medium with SmGM-2 Smooth Muscle Cell Growth Medium-2 Bullet Kit, excluding gentamicin sulfate. HCAMSCs were used between passage 5-6 for all experiments.  Once HCAMSCs reached 70% confluence in 6-well plates, serum-free media was applied for 24 hours prior to lentiviral transduction with polybrene. After overnight incubation, the medium was replaced with fresh full-serum SmGM medium. HCASMCs were harvested for RNA isolaion 48 hours later.   FastQ files were processed using nf-core/rnaseq (version 3.17.0) pipeline. Pre-processing to infer strandedness was performed using fq (v0.12.0) and 203 Salmon (v1.10.3).  RNA extraction was performed using the Zymo Quick-RNA Microprep Kit.  Following quality control, the NEBNext Ultra II Directional Kit was used for library preparation.
description
adult
developmental stage
normal
disease
TWIST1-TWIST1 homodimer
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
coronary artery
organism part
Homo sapiens
scientific_name
TWIST1-TWIST1 4
title