2025-10-30
ENA first public
ERC000011
ENA-CHECKLIST
PKUSZH
INSDC center name
public
INSDC status
ERS27100413
SRA accession
E-MTAB-15784:LCP94_TILs
Submitter Id
ArrayExpress
broker name
not collected
collection date
human
common name
Protocols: Patient lung cancer samples, including tumor tissues and adjacent normal tissues, were obtained from the Department of Thoracic Surgery at Peking University Shenzhen Hospital. Written informed consent was obtained from all participants prior to sample collection. All procedures were approved by the Ethics Committee of Peking University Shenzhen Hospital (NO.2020-057). Tissues were washed with DPBS containing 1% penicillin/streptomycin. Non-target tissues were manually removed before digestion. Nucleic acid extraction was performed as an integrated part of the single-cell library preparation protocol using the DNBelab C4 Single-Cell Library Prep Set (MGI). Within the microfluidic system, individual cells were encapsulated in droplets with barcoded beads. Cell lysis occurred within the droplets, and poly-adenylated mRNA molecules were captured onto the oligo-dT primers on the surface of the beads. Single-cell RNA-seq libraries were prepared using the DNBelab C4 Single-Cell Library Prep Set (MGI) according to the manufacturer's instructions. Following mRNA capture on barcoded beads, emulsion was broken and beads were recovered. Full-length cDNA was synthesized via reverse transcription. The resulting cDNA was then amplified to generate the final single-cell sequencing libraries.
description
IIIA
developmental stage
LUAD
disease
not collected
geographic location (country and/or sea)
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
TILs
sample type
Homo sapiens
scientific_name
female
sex
LCP94_TILs
title