2025-10-30
ENA first public
ERC000011
ENA-CHECKLIST
UMICH
INSDC center name
public
INSDC status
ERS26977415
SRA accession
E-MTAB-15689:Isolated Human Hepatocytes, HH003, PNPLA3-I148M
Submitter Id
ArrayExpress
broker name
hepatocyte
cell type
not collected
collection date
human
common name
Protocols: total RNA was extracted from isolated primary human hepatocytes using RNeasy Plus Micro Kit (Qiagen).  RNA integrity was assessed using the High Sensitivity RNA ScreenTape system on an Agilent 2200 TapeStation (Agilent). The SMART-Seq HT Kit (Takara Bio) was used to generate cDNA from 10 ng of total RNA, and the cDNA product was checked by an Agilent Fragment Analyzer system (Agilent) for quality control. The sequencing library was constructed by following the Illumina Nextera XT Sample Preparation Guide.
description
not collected
geographic location (country and/or sea)
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
liver
organism part
I148M
response to treatment
Homo sapiens
scientific_name
Isolated Human Hepatocytes, HH003, PNPLA3-I148M
title