2025-10-30
ENA first public
ERC000011
ENA-CHECKLIST
Department of Bioengineering UCLA
INSDC center name
public
INSDC status
ERS27070972
SRA accession
E-MTAB-15741:Untreated 2
Submitter Id
ArrayExpress
broker name
hematopoietic stem and progenitor cell
cell type
not collected
collection date
human
common name
Protocols: Wild type and KO Jurkat cells (American Type Culture Collection, Inc., ATCC) were cultured in 1× Roswell Park Memorial Institute Medium (RPMI) 1640. Total RNA was extracted and purified from HSPCs after cell lysis (~400,000 cells per condition in triplicates) using a Qiagen RNeasy Mini Kit.  RNA libraries for RNA-seq were prepared using the NEBNext Ultra II Directional RNA Library Prep with Sample Purification Beads following the manufacturer’s protocol which included reverse transcription of mRNA, ligation of adaptors, and PCR enrichment of libraries. Libary pool was 10nM.
description
adult
developmental stage
normal
disease
wild type genotype
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
peripheral blood
organism part
Homo sapiens
scientific_name
not applicable, rep 2
stimulus
Untreated 2
title