2025-10-30
ENA first public
ERC000011
ENA-CHECKLIST
The Second Hospital & Clinical Medical School, Lanzhou University
INSDC center name
public
INSDC status
ERS26976998
SRA accession
E-MTAB-15686:Sample 2
Submitter Id
ArrayExpress
broker name
HUVEC cell line
cell line
endothelial cells
cell type
not collected
collection date
human
common name
Protocols: The CRISPR-Pool SAM human library, containing 70290 sgRNAs targeting 23430 genes were constructed in MS2-P65-HSF1. After the cells were treated with apatinib for another week and their genomic DNA was extracted. Total DNA were extracted using NEBNext Ultra II DNA Kit The sgRNA fragments were amplified by PCR. After passing quality control, 20-50 ng of DNA fragments are used to construct a high-throughput sequencing library for the Illumina platform through steps such as End Repair, 5 Phosphorylation and dA-Tailing, Adapter Ligation, Clean Up, and PCR Enrichment and Clean Up.
description
adult
developmental stage
cancer
disease
wild type genotype
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
vasculature
organism part
Homo sapiens
scientific_name
Sample 2
title