2025-10-31
ENA first public
ERC000011
ENA-CHECKLIST
Institute of Oncology Research
INSDC center name
public
INSDC status
ERS27170485
SRA accession
E-MTAB-15933:Sample 9
Submitter Id
ArrayExpress
broker name
not collected
collection date
human
common name
Protocols: sample preparation began with gel digestion of muscle constructs using Trizol (TR118 MRC), followed by phase separation with 1-Bromo-3-chloropropane (B9673). RNA extraction from muscle cells was then performed according to ReliaPrep™ RNA Miniprep Systems’ protocol (Promega), which included DNase treatment. NEBNext UltraExpress RNA Library Prep Kit for Illumina (New England BioLabs Inc.) was employed with the NEBNext Multiplex Oligos for Illumina (New England BioLabs Inc.) and NEBNext® Poly(A) mRNA Magnetic Isolation Module for cDNA synthesis with the addition of barcode sequences.
description
not collected
geographic location (country and/or sea)
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
myobundles
organism part
1
replicate
Homo sapiens
scientific_name
Not stimulated
stimulus
D14
time point
Sample 9
title