2025-10-31
ENA first public
ERC000011
ENA-CHECKLIST
School of infection and Immunity, University of Glasgow
INSDC center name
public
INSDC status
ERS24832302
SRA accession
E-MTAB-15203:D3 Yoda1
Submitter Id
ArrayExpress
broker name
not collected
collection date
human
common name
Protocols: On the days of the extraction, the cells were washed with D-PBS in the 24 well plate. Then the cells were lysed using RLT buffer + 1% b-mercaptoethanol, at RT, mixing well.  PIEZO1 activation using Yoda1  Macrophages were differentiated from CD14 monocytes (from 6 healthy donors) as described for 6 days in complete RPMI supplemented with 20 ng/mL M-CSF, changing medium on day 3. On day 5 of differentiation, the cells were stimulated with 20 uM Yoda1, for 12 hours (overnight), before being lysed for RNA extraction.  RNA was extracted using RNeasy Microkit, following manufacture's instructions (Qiagen, 74004). Libraries were prepared by University of Glasgow, MVLS Shared Research Facility, for Illumina sequencing.
description
adult
developmental stage
not collected
geographic location (country and/or sea)
Cell culture monolayer
growth condition
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
Homo sapiens
scientific_name
male
sex
Yoda1
stimulus
D3 Yoda1
title