2025-10-31
ENA first public
ERC000011
ENA-CHECKLIST
PKUSZH
INSDC center name
public
INSDC status
ERS27100183
SRA accession
E-MTAB-15782:Sample 1
Submitter Id
30
year
age
ArrayExpress
broker name
acinar cell
cell type
not collected
collection date
Protocols: Patient lung cancer samples, including tumor tissues and adjacent normal tissues, were obtained from the Department of Thoracic Surgery at Peking University Shenzhen Hospital. Written informed consent was obtained from all participants prior to sample collection. All procedures were approved by the Ethics Committee of Peking University Shenzhen Hospital (NO.2020-057). Tissues were washed with DPBS containing 1% penicillin/streptomycin. Non-target tissues were manually removed before digestion. Nucleic acid extraction was performed as an integrated part of the single-cell library preparation protocol using the DNBelab C4 Single-Cell Library Prep Set (MGI). Within the microfluidic system, individual cells were encapsulated in droplets with barcoded beads. Cell lysis occurred within the droplets, and poly-adenylated mRNA molecules were captured onto the oligo-dT primers on the surface of the beads. Single-cell RNA-seq libraries were prepared using the DNBelab C4 Single-Cell Library Prep Set (MGI) according to the manufacturer's instructions. Following mRNA capture on barcoded beads, emulsion was broken and beads were recovered. Full-length cDNA was synthesized via reverse transcription. The resulting cDNA was then amplified to generate the final single-cell sequencing libraries.
description
II
developmental stage
lung cancer
disease
not collected
geographic location (country and/or sea)
11
individual
not applicable
isolate
Thermoplasma acidophilum DSM 1728
organism
Thermoplasma acidophilum DSM 1728
organism
vagal ganglion 1
organism part
Thermoplasma acidophilum DSM 1728
scientific_name
female
sex
Sample 1
title