2025-10-31
ENA first public
ERC000011
ENA-CHECKLIST
KU Leuven
INSDC center name
public
INSDC status
ERS21050972
SRA accession
E-MTAB-14455:EXT385
Submitter Id
15
week
age
ArrayExpress
broker name
uterine cell
cell_type
not collected
collection date
house mouse
common name
Protocols: Estrous cycle phase was determined by vaginal smear.14-15 weeks old mice were euthanized by CO2 asphyxiation, followed by uterine horn dissection. The uterine horns were longitudinally opened to expose the endometrium and cut into small pieces. Subsequently, these uterine horn pieces were dissociated enzymatically in 1% collagenase for 60 minutes at 37°C. Afterwards, the endometrial tissue pieces were mechanically dissociated by pipetting up and down for 10 minutes. 10% FBS was added to stop the enzymatic reaction. The cell suspension was filtered through a 40 μm cell strainer. The uterus single-cell suspensions were converted to barcoded Drop-seq libraries by using the Chromium Single Cell 3’ V3.1 Library, Gel Bead & Multiplex Kit and Chip Kit (10X Genomics; Pleasanton, California, USA), aiming for an estimated 8,000 cells per library.  Single-cell libraries were prepared with the Chromium Single Cell 3’ V3.1 Chemistry Library Kit, Gel Bead & Multiplex Kit and Chip Kit from 10X Genomics.
description
adult
developmental stage
Cystic fibrosis
disease
Estrus
estrous cycle phase
Cftr <F508del/del>
genotype
not collected
geographic location (country and/or sea)
EXT385
individual
not applicable
isolate
Mus musculus
organism
Mus musculus
organism
uterus
organism part
Mus musculus
scientific_name
female
sex
CFTR<tm1EUR>
strain
EXT385
title