2025-10-31
ENA first public
ERC000011
ENA-CHECKLIST
SAAS
INSDC center name
public
INSDC status
ERS27100169
SRA accession
E-MTAB-15781:zhuanjianlv-3
Submitter Id
12
year
age
ArrayExpress
broker name
not collected
collection date
Green Toon
cultivar
Protocols: Young tender leaflets from apical tips and fully expanded leaflets from basal positions of the same compound leaf were collected from three Toona sinensis cultivars exhibiting distinct leaf colors: red, brown, and green. Tissues were immediately frozen in liquid nitrogen and stored at -80°C until RNA extraction. Toona sinensis plants were grown under natural field conditions in a cultivation site in Beijing, China, with standard agricultural practices. Samples were collected during the spring growing season when leaf flushing occurred. Total RNA was extracted using the TRIzol reagent (Invitrogen) according to the manufacturer’s instructions. RNA concentration and purity were determined using a NanoDrop spectrophotometer (OD260/280 ratio), and integrity was assessed by agarose gel electrophoresis. Stranded RNA-seq libraries were constructed using the NEBNext Ultra II Directional RNA Library Prep Kit (New England Biolabs) following the standard protocol. Poly(A) selection was performed to enrich for mRNA. Libraries were amplified with 12 PCR cycles and size-selected for fragments around 300 bp. Library quality and concentration were validated using an Agilent Bioanalyzer.
description
Early germination stage
developmental stage
Green Toon
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Toona sinensis
organism
Toona sinensis
organism
leaf
organism part
Toona sinensis
scientific_name
zhuanjianlv-3
title