2025-10-31
ENA first public
ERC000011
ENA-CHECKLIST
Institute of Cardiovascular Regeneration
INSDC center name
public
INSDC status
ERS23823220
SRA accession
E-MTAB-14947:Sample 7
Submitter Id
72
week
age
ArrayExpress
broker name
cardiac muscle cell
cell_type
not collected
collection date
house mouse
common name
Protocols: Mouse heart tissues were immediately frozen in liquid nitrogen after OCT compound (Tissue-Tek) embedding. Ten-micrometer tissue cryosections were stained with hematoxylin and eosin (H&E) and the appropriate tissue regions were selected for further processing. Samples were integrated to identify shared spatial domains using STAligner. Frozen heart tissue samples were embedded in OCT compound (Tissue-Tek) and subsequently cryosectioned at 10 µm thickness using a Cryostar NX70 cryostat (Thermo Fisher Scientific). Tissue sections were immediately transferred onto pre-chilled Visium Tissue Optimization Slides (10X Genomics, PN-1000193) to determine the optimal permeabilization time. Following manufacturer recommendations (10X Genomics), optimization experiments established an ideal permeabilization window at either 12 or 18 minutes for efficient tissue digestion and RNA release. For spatial transcriptomics, tissue sections were placed onto Visium Spatial Gene Expression Slides (10X Genomics, PN-1000187) and processed according to the Visium Spatial Gene Expression User Guide. H&E staining was performed as suggested by 10X Genomics. Brightfield imaging of tissue sections was performed using a Fritz brightfield digital microscope (Precipoint). Image stitching and processing were conducted using NIS-Elements software (Nikon).
description
adult
developmental stage
wild type genotype
genotype
not collected
geographic location (country and/or sea)
old_2
individual
not applicable
isolate
Mus musculus
organism
Mus musculus
organism
heart
organism part
Mus musculus
scientific_name
female
sex
C57BI/6J
strain
Sample 7
title