2025-10-31
ENA first public
ERC000011
ENA-CHECKLIST
KAUST
INSDC center name
public
INSDC status
ERS21132340
SRA accession
E-MTAB-14525:HDMEC1-2h1T
Submitter Id
ArrayExpress
broker name
human dermal microvascular endothelial cell
cell_line
microvascular endothelial cell
cell_type
not collected
collection date
human
common name
Protocols: Samples were collected directly in the well using a lysing solution (1% 2-mercaptoethanol + Buffer RLT; RNeasy Mini Kit, 74106, Qiagen). Study 1: Human brain microvascular endothelial cells (HBMEC) and human dermal microvascular endothelial cells (HDMEC) were stimulated with either 0ng/mL, 1ng/mL or 10ng/mL of TNF for 0 hours, 10 minutes (0.167 hours), 30 minutes (0.5 hours), 2 hours, or 6 hours. Study 2: HBMEC were stimulated with either 1ng/mL or 10ng/mL of TNF for 0 hours, 6 hours, 12 hours, or 24 hours. RNeasy mini kit (Qiagen, 74106) buffers and protocol. RNA-sequencing libraries were prepared using the TruSeq Stranded mRNA kit (20020595, Illumina following the manufacturers protocol.
description
wild type genotype
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
dermis
organism part
Homo sapiens
scientific_name
HDMEC1-2h1T
title