2025-10-31
ENA first public
ERC000011
ENA-CHECKLIST
Temasek Life Sciences Laboratory
INSDC center name
public
INSDC status
ERS27149793
SRA accession
E-MTAB-15889:Sample 1
Submitter Id
ArrayExpress
broker name
not collected
collection date
Protocols: Samples were harvested via centrifugation, washed once in sterile MilliQ water, and snap-frozen in liquid nitrogen Cell suspensions were precultured in liquid Full Yamagami media for seven days prior to treatment Klebsormidium cultures were inoculated to liquid Full Yamagami media (pH5.5, 1% sucrose) containing the chemical of interest and grown for 48h at 80rpm, at 22°C, under 24h of low light. Frozen samples are milled in extraction buffer and total RNA extracted using the QIAGEN Plant RNeasy kit Directional mRNA library preparation (poly A enrichment)
description
NIES-2285
ecotype
wild type genotype
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Klebsormidium nitens
organism
Klebsormidium nitens
organism
whole organism
organism part
1
replicate
Klebsormidium nitens
scientific_name
none
stimulus
Sample 1
title