2025-11-01
ENA first public
ERC000011
ENA-CHECKLIST
a Celvia CC, Tartu, Estonia. b Department of Clinical Genetics, Maastricht University Medical Centre+, Maastricht, The Netherlands. c Department of Genetics and Cell Biology, GROW School for Oncology and Developmental Biology, Maastricht University, Maastricht, The Netherlands.
INSDC center name
public
INSDC status
ERS25497703
SRA accession
E-MTAB-15505:S11_tissue
Submitter Id
ArrayExpress
broker name
not collected
collection date
human
common name
ES
cycle phase
Protocols: The detailed sample collection criteria are described in Apostolov et al., 2025. According to the provided summary, paired transcriptomic samples of endometrial tissue and uterine fluid extracellular vesicles (UF-EVs) were obtained from 19 fertile, healthy women. These samples were collected across four specific phases of the menstrual cycle: proliferative (n=4), early-secretory (n=5), mid-secretory (n=5), and late-secretory (n=5). Additionally, two unpaired UF-EV samples were collected from two healthy donors, one in the mid-secretory and one in the late-secretory phase.  The nucleic acid library construction protocol began with RNA extraction using QIAzol and miRNeasy micro kits, a process which consecutively separated the large and small RNA fractions. Quality and quantity of the extracted RNA were then assessed; a Bioanalyzer TapeStation 2100 was used for general quality checks, while the small RNA fraction was specifically quantified using a Qubit microRNA assay. For library construction, different kits were used depending on the target. Small RNA libraries were generated from 20 ng of starting material using a NEXTflex small RNA library preparation kit v4. In contrast, mRNA libraries were constructed using the TruSeq exome RNA library preparation kit (Illumina). This method enriches for coding regions via sequence-specific probes and does not depend on a poly(A) tail. The starting amount for uterine fluid extracellular vesicle (UF-EV) samples was 100 ng of RNA, or the maximum available quantity. Once the libraries were complete, they were pooled and evaluated with a High Sensitivity DNA ScreenTape D1000.
description
not collected
geographic location (country and/or sea)
S11
individual
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
endometrium
organism part
Tissue
sampling site
Homo sapiens
scientific_name
female
sex
S11_tissue
title