2025-11-01
ENA first public
ERC000011
ENA-CHECKLIST
UCSD
INSDC center name
public
INSDC status
ERS27164367
SRA accession
E-MTAB-15894:N701_scK562_TNFA_Replication_RT_1
Submitter Id
ArrayExpress
broker name
K562
cell line
not collected
collection date
human
common name
Protocols: Automated cell sampling using ChronoSeq device. Each cell sample is injected with a unique injection of Time-Tagged ChronoSeq beads. ChronoSeq library preparation protocol is very similar to Drop-seq. Beads from all time-tags are pooled together, then split into four equal parts for Reverse Transcription followed by PCR amplification.
description
chronic myelogenous leukemia
disease
not collected
geographic location (country and/or sea)
430
microliter
input cell concentration
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
bone marrow
organism part
1
reverse transcription split
1
minute
sampling duration
10
minute
sampling interval
Homo sapiens
scientific_name
10
nanogram per milliliter
stimulus concentration
1->12
time-tag injection order
1 through 12
time-tags used
N701_scK562_TNFA_Replication_RT_1
title