2025-11-02
ENA first public
ERC000011
ENA-CHECKLIST
UCSD
INSDC center name
public
INSDC status
ERS27169485
SRA accession
E-MTAB-15928:N701_scTHP1_K562_LPS_Split_RT_1
Submitter Id
ArrayExpress
broker name
THP-1 and K562
cell line
not collected
collection date
human
common name
Protocols: Automated cell sampling using ChronoSeq device. Each cell sample is injected with a unique injection of Time-Tagged ChronoSeq beads. https://kanishkasthana.github.io/ChronoSeq/protocol_and_software_for_running_chronoseq_device.html For K562Cells grown in suspension in DMEM with 10% FBS and 1%PEN-STREP at 5%CO2 and 37C.  THP-1 cells were first grown in suspension same as K562 and then treated with 100nM PMA to induce differenciation for 48 hours. This was followed by a 72 hour rest period before harvesting with 10mM EDTA in DPBS. https://kanishkasthana.github.io/ChronoSeq/protocol_for_preparing_cells_AdditionalFiltration.html 1ug/ml LPS manually added to Cell Sample Reservoir 5 minutes after first sample/time-tag Oligo-DT beads were used to capture mRNA from lysed Single-Cells inside droplets. ChronoSeq library preparation protocol is very similar to Drop-seq. Beads from all time-tags are pooled together, then split into four equal parts for Reverse Transcription followed by PCR amplification. https://kanishkasthana.github.io/ChronoSeq/protocol_library_preparation_for_dropseq_chronoseq_beads.html
description
not collected
geographic location (country and/or sea)
430
microliter
input cell concentration
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
1
reverse transcription split
1
minute
sampling duration
15
minute
sampling interval
Homo sapiens
scientific_name
lipopolysaccharide(LPS)
stimulus
1
microgram per milliliter
stimulus concentration
1->12
time-tag injection order
N701_scTHP1_K562_LPS_Split_RT_1
title