2025-11-02
ENA first public
ERC000011
ENA-CHECKLIST
UCSD
INSDC center name
public
INSDC status
ERS27173424
SRA accession
E-MTAB-15956:N704_3TP_K562_EL4_TNFA_NxSEQ_430cells
Submitter Id
ArrayExpress
broker name
K562 + EL4
cell line
not collected
collection date
Protocols: Automated cell sampling using ChronoSeq device. Each cell sample is injected with a unique injection of Time-Tagged ChronoSeq beads. https://kanishkasthana.github.io/ChronoSeq/protocol_and_software_for_running_chronoseq_device.html K562 and EL4 cells grown in suspension in DMEM with 10% FBS and 1%PEN-STREP at 5%CO2 and 37C.  https://kanishkasthana.github.io/ChronoSeq/protocol_for_preparing_cells_AdditionalFiltration.html 10ng/ml TNF-α added to stimulate the cells. Added 5 minutes after first sample. Oligo-DT beads were used to capture mRNA from lysed Single-Cells inside droplets. ChronoSeq library preparation protocol is very similar to Drop-seq. Beads from all time-tags are pooled together, then split into four equal parts for Reverse Transcription followed by PCR amplification. https://kanishkasthana.github.io/ChronoSeq/protocol_library_preparation_for_dropseq_chronoseq_beads.html
description
not collected
geographic location (country and/or sea)
430
microliter
input cell concentration
not applicable
isolate
mixed sample
organism
mixed sample
organism
1
minute
sampling duration
10
minute
sampling interval
mixed sample
scientific_name
10ng/ml TNF-α
stimulus
10
nanogram per microliter
stimulus concentration
1->3
time-tag injection order
N704_3TP_K562_EL4_TNFA_NxSEQ_430cells
title