2025-11-02
ENA first public
ERC000011
ENA-CHECKLIST
UCSD
INSDC center name
public
INSDC status
ERS27172560
SRA accession
E-MTAB-15947:N702_scK562_TNFA_Puromycin_RT3
Submitter Id
ArrayExpress
broker name
K562
cell line
not collected
collection date
human
common name
Protocols: Automated cell sampling using ChronoSeq device. Each cell sample is injected with a unique injection of Time-Tagged ChronoSeq beads. https://kanishkasthana.github.io/ChronoSeq/protocol_and_software_for_running_chronoseq_device.html K562 cells grown in suspension in DMEM with 10% FBS and 1%PEN-STREP at 5%CO2 and 37C.  https://kanishkasthana.github.io/ChronoSeq/protocol_for_preparing_cells_AdditionalFiltration.html 10ng/ml TNF-α + 5μg/ml Puromycin were added to stimulate the cells and block translation respectively. Both were added together 5 minutes after first sample. Oligo-DT beads were used to capture mRNA from lysed Single-Cells inside droplets. ChronoSeq library preparation protocol is very similar to Drop-seq. Beads from all time-tags are pooled together, then split into four equal parts for Reverse Transcription followed by PCR amplification. https://kanishkasthana.github.io/ChronoSeq/protocol_library_preparation_for_dropseq_chronoseq_beads.html
description
chronic myelogenous leukemia
disease
not collected
geographic location (country and/or sea)
500
microliter
input cell concentration
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
bone marrow
organism part
3
reverse transcription split
1
minute
sampling duration
10
minute
sampling interval
Homo sapiens
scientific_name
TNF-α + Puromycin
stimulus
10ng/ml TNF-α + 5μg/ml Puromycin
stimulus concentration
1->12
time-tag injection order
N702_scK562_TNFA_Puromycin_RT3
title