2025-11-02
ENA first public
ERC000011
ENA-CHECKLIST
Laboratory of Traslational Research. Azienda USL-IRCCS di Reggio Emilia
INSDC center name
public
INSDC status
ERS18345620
SRA accession
E-MTAB-13851:TLBR2_CT_2
Submitter Id
ArrayExpress
broker name
TLBR2
cell_line
T cell
cell_type
not collected
collection date
human
common name
Protocols: 50'000 cells were collected for ATAC-seq. Cell pellet was lysed in ATAC-seq Lysis Buffer then diluted in ATAC-seq Wash Buffer.  Tagmentation was performed with Illumina Tagment DNA (TD) TDE1 Enzyme and Buffer (Illumina). Nuclear pellet was resuspended in Transposition Mix and incubated at 37°C. Cells were cultured in RPMI medium supplemented with 10% FBS, 1% P/S and 20U IL-2. Functional HELLS knockdown by shRNA was induced by the treatment with Doxycycline (DOX) for 48h at final concentration of 100ng/ml. DNA was purified using DNA Clean and Concentrator-5 KIT (Zymo Research). Fragmented DNA amplification and barcoding was performed with NEBNext Ultra II Q5 2X Master Mix (New England Biolabs) with sample-specific adapters. Samples were quantified with NEBNext Library Quantification kit (New England Biolabs) and amplified for an additional 7 cycles.
description
adult
developmental stage
Anaplastic Large Cell Lymphoma, ALK-Negative
disease
inducible shRNA against HELLS
genetic modification
wild type genotype
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
breast
organism part
2
replicate
Homo sapiens
scientific_name
TLBR2_CT_2
title