2025-11-03
ENA first public
ERC000013
ENA-CHECKLIST
University of Bremen
INSDC center name
public
INSDC status
ERS26752653
SRA accession
222201:fc3fdc59-5216-46fd-bd4d-296f51cc7549
Submitter Id
laboratory environment [ENVO:01001405]
broad-scale environmental context
GFBIO
broker name
9.34
carbs_per100g
low
category_sugar_per_100g
2021-02-11
collection date
5
count_transfer_microcosm
ITS DNA amplicon sequencing of fecal material collected from 30 female flies raised in onion substrate based microcosms after the 5th microcosm transfer event. For microcosms microbiota were initially collected from the field on a variety of substrates in 2020 and kept under standard rearing condition with onion provided ad libitum. Flies developing in these microcosms were offered fresh substrate egglaying oppertunities roughly monthly marking also a microbiota transfer event.
description
excretion [GO:0007588]
environmental medium
host associated
environmental package
2021-08-10
extraction_date
Fink et al. 2040
extraction_method
Germany
geographic location (country and/or sea)
53.11205
DD
geographic location (latitude)
8.852764
DD
geographic location (longitude)
Bremen
geographic location (region and locality)
yes
gravidity
host associated
host associated environmental package
fecal material
host body habitat
stool
host body product
fruit fly
host common name
Onion fed ad libitum dry yeast-sucrose mix and water
host diet
outbred
host genotype
20 °C 16:8 light-dark cycl
host growth conditions
imago
host life stage
Microcosms supplied with Onion ad libitum.
host substrate
7227
host taxid
mimarks-survey
investigation type
AGGAGRBTTTCTTTTCCTCCGCT
its_primersequence_forward
TCATGATGAAGAACGYAGYRAA
its_primersequence_reverse
40
kcal_per100g
microcosm [ENVO:01000621]
local environmental context
Onion
microcosm_substrate_basis
Fink et al. 2017
nucleic acid extraction
symbiont
observed biotic relationship
feces metagenome
organism
ITS3_KYO2 (forward)  'AGGAGRBTTTCTTTTCCTCCGCT' and ITS4_KY03  'TCATGATGAAGAACGYAGYRAA' (Toju et al. 2012)
pcr primers
fecal and ontogenetic environment microbiota of Drosophila melanogaster from different fruit based microcosms
project name
X260
row_names
screw cap device 15 mL
sample collection device
10.21769/BioProtoc.2547
sample collection method
-80
°C
sample storage temperature
24-G5-Onion
sample_code
ITS DNA amplicon sequencing of fecal material collected from 30 female flies raised in onion substrate based microcosms after the 5th microcosm transfer event. For microcosms microbiota were initially collected from the field on a variety of substrates in 2020 and kept under standard rearing condition with onion provided ad libitum. Flies developing in these microcosms were offered fresh substrate egglaying oppertunities roughly monthly marking also a microbiota transfer event.
sample_description
260
sample_id
Onion 2020 G5
sample_label
24
sample_no
fecal
sample_type
2021-02-11
sampling_date
feces metagenome
scientific_name
Illumina MiSeq
sequencing method
O-G5
short_label
Microcosms were set-up from field samples collected at the Biogarten facilities of the University of Bremen Germany. They were kept under standard rearing conditions in the laboratories of University of Bremen.  From these microcoms fecal samples were taken from groups of gravid Drosophila melanogaster and ontogenetic environments for amplicon sequencing of 16S and ITS region. Additional controls for the standard population simple transfer to fruits and blank samples are included.
short_study_descriptor
true
submitted to insdc
Onion
substrate_type
1103364
substrate_usda_fdc_no
4.24
sugar_100g
5.8S-LSU
target subfragment
20
ºC
temperature
FlyFeces-24-G5-Onion-ITS
title