TACACGACGCTCTTCCGATCTTCATCCTACGGGNGGCWGCAG
16s_primersequence_forward
CAGACGTGTGCTCTTCCGATCCGCTCAGACTACHVGGGTATCTAATCC
16s_primersequence_reverse
2025-11-03
ENA first public
ERC000013
ENA-CHECKLIST
University of Bremen
INSDC center name
public
INSDC status
ERS26748239
SRA accession
222081:355a01cb-e43d-4209-970a-690da72cff7b
Submitter Id
laboratory environment [ENVO:01001405]
broad-scale environmental context
GFBIO
broker name
2021-03-06
collection date
16S DNA amplicon sequencing of fecal material collected from 30 female flies raised on a standard laboratory diet based on cornstrach apple sauce dry yeast and agar.
description
excretion [GO:0007588]
environmental medium
host associated
environmental package
Germany
geographic location (country and/or sea)
53.11205
DD
geographic location (latitude)
8.852764
DD
geographic location (longitude)
Bremen
geographic location (region and locality)
yes
gravidity
host associated
host associated environmental package
fecal material
host body habitat
stool
host body product
fruit fly
host common name
Cornstrach-medium fed ad libitum dry yeast-sucrose mix and water
host diet
outbred
host genotype
20 °C 16:8 light-dark cycle
host growth conditions
imago
host life stage
Standard laboratory diet based on cornstarch.
host substrate
7227
host taxid
mimarks-survey
investigation type
microcosm [ENVO:01000621]
local environmental context
Fink et al. 2017
nucleic acid extraction
symbiont
observed biotic relationship
feces metagenome
organism
341F 'TACACGACGCTCTTCCGATCTTCATCCTACGGGNGGCWGCAG' and 785R 'CAGACGTGTGCTCTTCCGATCCGCTCAGACTACHVGGGTATCTAATCC' (Klindworth et al. 2013)
pcr primers
fecal and ontogenetic environment microbiota of Drosophila melanogaster from different fruit based microcosms
project name
X217
row_names
screw cap device 15 mL
sample collection device
10.21769/BioProtoc.2547
sample collection method
-80
°C
sample storage temperature
16S DNA amplicon sequencing of fecal material collected from 30 female flies raised on a standard laboratory diet based on cornstrach apple sauce dry yeast and agar.
sample_description
217
sample_id
25
sample_no
feces metagenome
scientific_name
Illumina MiSeq
sequencing method
Microcosms were set-up from field samples collected at the Biogarten facilities of the University of Bremen Germany. They were kept under standard rearing conditions in the laboratories of University of Bremen.  From these microcoms fecal samples were taken from groups of gravid Drosophila melanogaster and ontogenetic environments for amplicon sequencing of 16S and ITS region. Additional controls for the standard population
short_study_descriptor
true
submitted to insdc
V3-V4 region
target subfragment
20
ºC
temperature
FlyFeces-25-lab-diet-1-16S
title