2025-11-03
ENA first public
ERC000013
ENA-CHECKLIST
University of Bremen
INSDC center name
public
INSDC status
ERS26752640
SRA accession
222214:fc3fdc59-5216-46fd-bd4d-296f51cc7549
Submitter Id
laboratory environment [ENVO:01001405]
broad-scale environmental context
GFBIO
broker name
14.05
carbs_per100g
medium
category_sugar_per_100g
2021-06-30/2021-07-21
collection date
ITS DNA amplicon sequencing of surface scrape sample collected from 10  vials haboring a single larvae until eclosion. Vials are based on a apple substrate and inoculated with the allochthonous microbiota from Sample FlyFeces-31-G21-Tomato-ITS (also including bacteria). Then one single axenic Drosphila melanogaster larva was placed in each vial to develop; adult flies were removed and samples frozen before they were pooled. They may reflect fly associated communities of ontogenetic environment.
description
pulp [BTO:0001142]
environmental medium
host associated
environmental package
2021-08-11
extraction_date
Qiagen DNeasy PowerSoil Kit
extraction_method
Germany
geographic location (country and/or sea)
53.11205
DD
geographic location (latitude)
8.852764
DD
geographic location (longitude)
Bremen
geographic location (region and locality)
no
gravidity
host associated
host associated environmental package
ontogenetic environment
host body habitat
ontogenetic environment
host body product
fruit fly
host common name
1 mL of Apple substrate and microbiota from Tomato 2019 G27
host diet
outbred
host genotype
20 °C 16:8 light-dark cycl
host growth conditions
imago
host life stage
1 mL of Apple substrate and microbiota from Tomato 2019 G27
host substrate
7227
host taxid
Tomato 2019 G27
inoculate
mimarks-survey
investigation type
AGGAGRBTTTCTTTTCCTCCGCT
its_primersequence_forward
TCATGATGAAGAACGYAGYRAA
its_primersequence_reverse
54
kcal_per100g
microcosm [ENVO:01000621]
local environmental context
Qiagen Power Soil kit according to manufacturer's protocol
nucleic acid extraction
symbiont
observed biotic relationship
feces metagenome
organism
ITS3_KYO2 (forward)  'AGGAGRBTTTCTTTTCCTCCGCT' and ITS4_KY03  'TCATGATGAAGAACGYAGYRAA' (Toju et al. 2012)
pcr primers
fecal and ontogenetic environment microbiota of Drosophila melanogaster from different fruit based microcosms
project name
X273
row_names
collection tube 2 mL
sample collection device
substrate samples  were taken in pools of 10 after fly eclosion in a apple environment inoculated with fecal material of flies raised in tomato microcosm.
sample collection method
-80
°C
sample storage temperature
37-A3-sub
sample_code
ITS DNA amplicon sequencing of surface scrape sample collected from 10  vials haboring a single larvae until eclosion. Vials are based on a apple substrate and inoculated with the allochthonous microbiota from Sample FlyFeces-31-G21-Tomato-ITS (also including bacteria). Then one single axenic Drosphila melanogaster larva was placed in each vial to develop; adult flies were removed and samples frozen before they were pooled. They may reflect fly associated communities of ontogenetic environment.
sample_description
273
sample_id
Apple post eclosion
sample_label
37
sample_no
substrate
sample_type
"2021-06-30/2021-07-21"
sampling_date
feces metagenome
scientific_name
Illumina MiSeq
sequencing method
A3-sub
short_label
Microcosms were set-up from field samples collected at the Biogarten facilities of the University of Bremen Germany. They were kept under standard rearing conditions in the laboratories of University of Bremen.  From these microcoms fecal samples were taken from groups of gravid Drosophila melanogaster and ontogenetic environments for amplicon sequencing of 16S and ITS region. Additional controls for the standard population simple transfer to fruits and blank samples are included.
short_study_descriptor
true
submitted to insdc
Apple
substrate_type
577849
substrate_usda_fdc_no
10.33
sugar_100g
5.8S-LSU
target subfragment
20
ºC
temperature
Apple post eclosion-37-inocullated-FlyFeces-31-G21-Tomato-ITS
title