TACACGACGCTCTTCCGATCTTCATCCTACGGGNGGCWGCAG
16s_primersequence_forward
CAGACGTGTGCTCTTCCGATCCGCTCAGACTACHVGGGTATCTAATCC
16s_primersequence_reverse
2025-11-03
ENA first public
ERC000013
ENA-CHECKLIST
University of Bremen
INSDC center name
public
INSDC status
ERS26748234
SRA accession
222086:355a01cb-e43d-4209-970a-690da72cff7b
Submitter Id
laboratory environment [ENVO:01001405]
broad-scale environmental context
GFBIO
broker name
none
carbs_per100g
none
category_sugar_per_100g
2021-02-11
collection date
0
count_transfer_microcosm
16S DNA amplicon sequencing of empty screw cap tubes handled alongside fecal material sampling as a control for environmental contaminants.
description
microcosm [ENVO:01000621]
environmental medium
host associated
environmental package
2021-08-13
extraction_date
Fink et al. 2046
extraction_method
Germany
geographic location (country and/or sea)
53.11205
DD
geographic location (latitude)
8.852764
DD
geographic location (longitude)
Bremen
geographic location (region and locality)
control sample
gravidity
host associated
host associated environmental package
control sample
host body habitat
control sample
host body product
control sample
host common name
control sample
host diet
control sample
host genotype
control sample
host growth conditions
control sample
host life stage
control sample
host substrate
7227
host taxid
mimarks-survey
investigation type
none
kcal_per100g
microcosm [ENVO:01000621]
local environmental context
Fink et al. 2017
nucleic acid extraction
symbiont
observed biotic relationship
feces metagenome
organism
341F 'TACACGACGCTCTTCCGATCTTCATCCTACGGGNGGCWGCAG' and 785R 'CAGACGTGTGCTCTTCCGATCCGCTCAGACTACHVGGGTATCTAATCC' (Klindworth et al. 2013)
pcr primers
fecal and ontogenetic environment microbiota of Drosophila melanogaster from different fruit based microcosms
project name
X222
row_names
screw cap device 15 mL
sample collection device
10.21769/BioProtoc.2547
sample collection method
-80
°C
sample storage temperature
30-blank-2
sample_code
16S DNA amplicon sequencing of empty screw cap tubes handled alongside fecal material sampling as a control for environmental contaminants.
sample_description
222
sample_id
Blank
sample_label
30
sample_no
none
sample_type
2021-02-11
sampling_date
feces metagenome
scientific_name
Illumina MiSeq
sequencing method
blank-2
short_label
Microcosms were set-up from field samples collected at the Biogarten facilities of the University of Bremen Germany. They were kept under standard rearing conditions in the laboratories of University of Bremen.  From these microcoms fecal samples were taken from groups of gravid Drosophila melanogaster and ontogenetic environments for amplicon sequencing of 16S and ITS region. Additional controls for the standard population simple transfer to fruits and blank samples are included.
short_study_descriptor
true
submitted to insdc
Blank
substrate_type
none
substrate_usda_fdc_no
none
sugar_100g
V3-V4 region
target subfragment
20
ºC
temperature
FlyFeces-collection-30-blank-2-16S
title