TACACGACGCTCTTCCGATCTTCATCCTACGGGNGGCWGCAG
16s_primersequence_forward
CAGACGTGTGCTCTTCCGATCCGCTCAGACTACHVGGGTATCTAATCC
16s_primersequence_reverse
2025-11-03
ENA first public
ERC000013
ENA-CHECKLIST
University of Bremen
INSDC center name
public
INSDC status
ERS26748262
SRA accession
222058:355a01cb-e43d-4209-970a-690da72cff7b
Submitter Id
laboratory environment [ENVO:01001405]
broad-scale environmental context
GFBIO
broker name
78.57
carbs_per100g
high
category_sugar_per_100g
2020-09-28
collection date
12
count_transfer_microcosm
16S DNA amplicon sequencing of fecal material collected from 30 female flies raised in banana substrate based microcosms after the 12th microcosm transfer event. For microcosms microbiota were initially collected from the field on a variety of substrates in 2019 and kept under standard rearing condition with banana provided ad libitum. Flies developing in these microcosms were offered fresh substrate egglaying oppertunities roughly monthly marking also a microbiota transfer event.
description
excretion [GO:0007588]
environmental medium
host associated
environmental package
2021-08-13
extraction_date
Fink et al. 2018
extraction_method
Germany
geographic location (country and/or sea)
53.11205
DD
geographic location (latitude)
8.852764
DD
geographic location (longitude)
Bremen
geographic location (region and locality)
yes
gravidity
host associated
host associated environmental package
fecal material
host body habitat
stool
host body product
fruit fly
host common name
Banana fed ad libitum dry yeast-sucrose mix and water
host diet
outbred
host genotype
20 °C 16:8 light-dark cycle
host growth conditions
imago
host life stage
Microcosms supplied with Banana ad libitum.
host substrate
7227
host taxid
mimarks-survey
investigation type
336
kcal_per100g
microcosm [ENVO:01000621]
local environmental context
Banana
microcosm_substrate_basis
Fink et al. 2017
nucleic acid extraction
symbiont
observed biotic relationship
feces metagenome
organism
341F 'TACACGACGCTCTTCCGATCTTCATCCTACGGGNGGCWGCAG' and 785R 'CAGACGTGTGCTCTTCCGATCCGCTCAGACTACHVGGGTATCTAATCC' (Klindworth et al. 2013)
pcr primers
fecal and ontogenetic environment microbiota of Drosophila melanogaster from different fruit based microcosms
project name
X194
row_names
screw cap device 15 mL
sample collection device
10.21769/BioProtoc.2547
sample collection method
-80
°C
sample storage temperature
2-G12-Banana
sample_code
16S DNA amplicon sequencing of fecal material collected from 30 female flies raised in banana substrate based microcosms after the 12th microcosm transfer event. For microcosms microbiota were initially collected from the field on a variety of substrates in 2019 and kept under standard rearing condition with banana provided ad libitum. Flies developing in these microcosms were offered fresh substrate egglaying oppertunities roughly monthly marking also a microbiota transfer event.
sample_description
194
sample_id
Banana 2019 G12
sample_label
2
sample_no
fecal
sample_type
2020-09-28
sampling_date
feces metagenome
scientific_name
Illumina MiSeq
sequencing method
B-G12
short_label
Microcosms were set-up from field samples collected at the Biogarten facilities of the University of Bremen Germany. They were kept under standard rearing conditions in the laboratories of University of Bremen.  From these microcoms fecal samples were taken from groups of gravid Drosophila melanogaster and ontogenetic environments for amplicon sequencing of 16S and ITS region. Additional controls for the standard population simple transfer to fruits and blank samples are included.
short_study_descriptor
true
submitted to insdc
Banana
substrate_type
362759
substrate_usda_fdc_no
57.14
sugar_100g
V3-V4 region
target subfragment
20
ºC
temperature
FlyFeces-2-G12-Banana-16S
title