2025-11-04
ENA first public
ERC000011
ENA-CHECKLIST
UCSD
INSDC center name
public
INSDC status
ERS27176923
SRA accession
E-MTAB-15966:N706_Bulk_K562_Control_600_Cells
Submitter Id
ArrayExpress
broker name
600
microliter
cell concentration
K562
cell line
not collected
collection date
human
common name
Protocols: Cells were automatically samples using the ChronoSeq device. 10μl of Time-Tagged beads at 450 beads/μl suspended in lysis buffer were directly mixed with human cells at 600cells/μl. In order 1->12 K562 grown in suspension in DMEM with 10% FBS and 1%PEN-STREP at 5%CO2 and 37C.  https://kanishkasthana.github.io/ChronoSeq/protocol_for_preparing_cells_AdditionalFiltration.html Oligo-DT beads were used to capture mRNA from lysed Single-Cells inside droplets. Beads are washed once with 6X SSC buffer and then twice with 1.25X RT buffer. Beads were then pooled together for a single RT reaction. very similar to Drop-seq. https://kanishkasthana.github.io/ChronoSeq/protocol_library_preparation_for_dropseq_chronoseq_beads.html
description
not collected
geographic location (country and/or sea)
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
10
minute
sampling interval
Homo sapiens
scientific_name
1->12
time-tag injection order
N706_Bulk_K562_Control_600_Cells
title