2025-11-04
ENA first public
ERC000011
ENA-CHECKLIST
UCSD
INSDC center name
public
INSDC status
ERS27176476
SRA accession
E-MTAB-15964:SC_3TP_QC_K562_EL4
Submitter Id
ArrayExpress
broker name
EL4+K562, K562, EL4
cell line
not collected
collection date
Protocols: K562+EL4 (Time Tag 10), K562(Time Tag 11), EL4 (Time Tag 12). Automated cell sampling using ChronoSeq device. Each cell sample is injected with a unique injection of Time-Tagged ChronoSeq beads. https://kanishkasthana.github.io/ChronoSeq/protocol_and_software_for_running_chronoseq_device.html K562 and EL4 cells grown in suspension in DMEM with 10% FBS and 1%PEN-STREP at 5%CO2 and 37C.  https://kanishkasthana.github.io/ChronoSeq/protocol_for_preparing_cells_AdditionalFiltration.html Oligo-DT beads were used to capture mRNA from lysed Single-Cells inside droplets. ChronoSeq library preparation protocol is very similar to Drop-seq. Beads from all time-tags are pooled together for Reverse Transcription followed by PCR amplification. https://kanishkasthana.github.io/ChronoSeq/protocol_library_preparation_for_dropseq_chronoseq_beads.html
description
not collected
geographic location (country and/or sea)
215
microliter
input cell concentrations
not applicable
isolate
mixed sample
organism
mixed sample
organism
1
minute
sampling duration
7
minute
sampling interval
mixed sample
scientific_name
10->12
time-tag injection order
SC_3TP_QC_K562_EL4
title