2025-11-05
ENA first public
ERC000014
ENA-CHECKLIST
MV
INSDC center name
public
INSDC status
ERS24936886
SRA accession
ena-SAMPLE-TAB-12-06-2025-10:00:56:946-45412
Submitter Id
fwd: AATGATACGGCGACCACCGAGATCTACACGCTTATGGTAATTGT, rev: CAAGCAGAAGACGGCATACGAGATAGTCAGCCAGCC
adapters
city [ENVO:00000856]; human settlement [ENVO:01001829]
broad-scale environmental context
2023-10-23
collection date
human stool metagenome analyzed with 16S amplicon sequencing
description
feces [UBERON:0001988]
environmental medium
Brazil
geographic location (country and/or sea)
-23
DD
geographic location (latitude)
-46
DD
geographic location (longitude)
Sao Paulo
geographic location (region and locality)
Feces [FMA:64183]; feces [UBERON:0001988]
host body product
UASP40101
host subject id
CC-BY-NC license issued by Christian_Hoffmann
license information
human body [FMA:20394]; feces [UBERON:0001988]
local environmental context
AATGATACGGCGACCACCGAGATCTACACGCT
multiplex identifiers
https://dx.doi.org/10.17504/protocols.io.kqdg3dzzl25z/v2
nucleic acid amplification
Maxwell RSC PureFood GMO and Authentication Kit AS1600
nucleic acid extraction
human gut metagenome
organism
initial denaturation:94degC_3min; denaturation:94degC_45s; annealing:50degC_60s; extension:72degC_90s; final extension:72degC_10min;
pcr conditions
fwd: GTGYCAGCMGCCGCGGTAA, rev: GGACTACNVGGGTWTCTAAT
pcr primers
Urban Agriculture Sao Paulo
project name
The Microbiota Vault
sample biobank
Christian_Hoffmann
sample coordinator
0000-0003-1736-8007
sample coordinator ORCID
USP, School Of Pharmaceutical Sciences
sample coordinator affiliation
-80
sample shipping temperature
P1M22D
sample storage duration
-80
°C
sample storage temperature
4
°C
sample transport temperature
human gut metagenome
scientific_name
run_404
sequencing_run
16S
target gene
V4
target subfragment
40101S00
title