2025-11-05
ENA first public
ERC000014
ENA-CHECKLIST
MV
INSDC center name
public
INSDC status
ERS24934968
SRA accession
ena-SAMPLE-TAB-11-06-2025-15:02:09:403-41726
Submitter Id
fwd: AATGATACGGCGACCACCGAGATCTACACGCTTATGGTAATTGT, rev: CAAGCAGAAGACGGCATACGAGATAGTCAGCCAGCC
adapters
not applicable
broad-scale environmental context
not applicable
collection date
all the volume of the PCR reaction was used for pooling. DNA extraction negative control (all dna extraction buffers without sample)
description
not applicable
environmental medium
not applicable
geographic location (country and/or sea)
not applicable
DD
geographic location (latitude)
not applicable
DD
geographic location (longitude)
not applicable
geographic location (region and locality)
not applicable
host body product
not applicable
host subject id
not applicable
license information
not applicable
local environmental context
AATGATACGGCGACCACCGAGATCTACACGCT
multiplex identifiers
https://dx.doi.org/10.17504/protocols.io.kqdg3dzzl25z/v2
nucleic acid amplification
Maxwell RSC PureFood GMO and Authentication Kit AS1600
nucleic acid extraction
unidentified
organism
initial denaturation:94degC_3min; denaturation:94degC_45s; annealing:50degC_60s; extension:72degC_90s; final extension:72degC_10min;
pcr conditions
fwd: GTGYCAGCMGCCGCGGTAA, rev: GGACTACNVGGGTWTCTAAT
pcr primers
Urban Agriculture Sao Paulo
project name
not applicable
sample biobank
not applicable
sample coordinator
not applicable
sample coordinator ORCID
not applicable
sample coordinator affiliation
not applicable
sample storage duration
unidentified
scientific_name
run_401
sequencing_run
16S
target gene
V4
target subfragment
20240131_PCRNEG
title