2025-11-05
ENA first public
ERC000011
ENA-CHECKLIST
MV
INSDC center name
public
INSDC status
ERS25080768
SRA accession
ena-SAMPLE-TAB-25-06-2025-10:48:49:510-46485
Submitter Id
fwd: AATGATACGGCGACCACCGAGATCTACACGCTTATGGTAATTGT, rev: CAAGCAGAAGACGGCATACGAGATAGTCAGCCAGCC
adapters
0.001
L
amount or size of sample collected
Coastal settlement
broad-scale environmental context
2022-12-06T09:00:00Z
collection date
human stool metagenome analyzed with 16S amplicon sequencing
description
No
drug usage
Faecal matter
environmental medium
Ahanta
ethnicity
Ghana
DD
geographic location (country and/or sea)
5
geographic location (latitude)
-2
DD
geographic location (longitude)
Ahanta West
geographic location (region and locality)
11
years
host age
Stool
host body product
Healthy
host disease status
141.7
cm
host height
Student
host occupation
Homo sapiens
host scientific name
Female
host sex
013
host subject id
31.1
kg
host total mass
CC BY license issued by Amma_Aboagyewa_Larbi
license information
Lavatories; beach
local environmental context
AATGATACGGCGACCACCGAGATCTACACGCT
multiplex identifiers
https://dx.doi.org/10.17504/protocols.io.kqdg3dzzl25z/v2
nucleic acid amplification
Maxwell RSC PureFood GMO and Authentication Kit AS1600
nucleic acid extraction
human gut metagenome
organism
initial denaturation:94degC_3min; denaturation:94degC_45s; annealing:50degC_60s; extension:72degC_90s; final extension:72degC_10min;
pcr conditions
fwd: GTGYCAGGCCGCGGTAA, rev: GGACTACNVGGGTWTCTAAT
pcr primers
Filaria-Malaria coinfection and gut microbiome
project name
The Microbiota Vault
sample biobank
Stool container
sample collection device
Amma_Aboagyewa_Larbi
sample coordinator
0000-0002-3814-6924
sample coordinator ORCID
KNUST, Kumasi, Department of biochemistry and biotechnology
sample coordinator affiliation
-78
°C
sample shipping temperature
P0Y10M
sample storage duration
KCCR, KNUST
sample storage location
-80
°C
sample storage temperature
-20
°C
sample transport temperature
8
hours
sample transport time
0.15
mL
sample volume or weight for DNA extraction
human gut metagenome
scientific_name
software
sequence quality check
run_405
sequencing_run
non-smoker
smoker
Cross-sectional
study design
16S
target gene
V4
target subfragment
FilMal_AKA013
title