2025-11-06
ENA first public
ERC000011
ENA-CHECKLIST
Hasthi Ram, NIPGR
INSDC center name
public
INSDC status
ERS23016947
SRA accession
SAMIN0010331-The CRISPR-Cas9 library approach can help to efficiently generate higher-order knockout mutant population
Submitter Id
IBDC
broker name
2023-08-15
collection date
Asian cultivated rice
common name
For NGS amplicon sequencing, plasmid pools were individually restriction digested using MfeI and SbfI enzymes to obtain a 257bp DNA fragment containing sgRNA sequence. The obtained 257 bp linear DNA fragments were pooled together proportionally and sequenced using paired-end 150 bp amplicon sequencing on the Illumina platform.
description
India
geographic location (country and/or sea)
Delhi
geographic location (region and locality)
Oryza sativa
organism
Oryza sativa
scientific_name
The CRISPR-Cas9 library approach can help to efficiently generate higher-order knockout mutant population
title