2025-11-07
ENA first public
ERC000011
ENA-CHECKLIST
SAMEA114590493
External Id
I. Medical Department Bioinformatics Core
INSDC center name
2023-11-07T13:12:19Z
INSDC last update
public
INSDC status
ERS16690640
SRA accession
E-MTAB-13522:S4
Submitter Id
8 to 12
week
age
ArrayExpress
broker name
not collected
collection date
house mouse
common name
Protocols: To isolate lamina propria cells, colons were removed from mice, cut open longitudinally, and washed in PBS, cut into 4 to 5 smaller pieces, then incubated in 20 mL HBSS containing 200 µL EDTA 0.5 M per sample at 37 °C in a shaker for 20 min to detach epithelial cells. After this first digest, the colon pieces were washed in PBS 3-5 times to remove the epithelial cells. The remaining pieces were then minced to disrupt the tissue and digested in 5 mL HBSS containing 2 % FCS, 3 µL DNaseI and 5 mg collagenase VIII per sample for 45 min at 37 °C on a shaker. After digestion, the suspension was mashed through a 100 µm cell strainer, then filtered again through a 40 µm cell strainer. The filtered suspension was centrifuged at 300 x g for 5 min at 4 °C to pellet the cells, which were then used for further applications. Samples were first lysed using the QiaShredder Kit (Qiagen), and RNA was isolated using the RNeasy Mini Kit (Qiagen). Library preparation and transcriptome sequencing was performed using 100base/paired-end reads on BGI’s DNBSEQTM Technology Platform.
description
adult
developmental stage
Nmes1 knockout
genotype
not collected
geographic location (country and/or sea)
control
il4 treatment
4
individual
not applicable
isolate
Mus musculus
organism
Mus musculus
organism
colon
organism part
Mus musculus
scientific_name
male
sex
C57Bl/6J
strain
S4
title