2025-11-08
ENA first public
ERC000011
ENA-CHECKLIST
CSIR-Centre for Cellular & Molecular Biology
INSDC center name
public
INSDC status
ERS22181950
SRA accession
E-MTAB-14608:SCD3HT29_KD2_S11
Submitter Id
ArrayExpress
broker name
HT-29 DZIP3 shRNA
cell_line
epithelial cell
cell_type
not collected
collection date
human
common name
Protocols: HT-29 control and DZIP3 KD cells were collected from 3 different wells for each group in a 6 well plate RNA was extracted from HT-29 cells(three biological replicates) using RNeasy mini kit (QIAGEN, #74104). The quality and quantity of total RNA was checked using agarose gel and Qubit 4.0. After assessing the quality of RNA, 800-900 ng of total RNA was subjected to NEBNext® ultra™ directional RNA library prep kit for Illumina® (#E7420) using NEBNextPoly(A) mRNA magnetic isolation module (NEB #E7490). The QC of the prepared library was performed using High-Sensitivity Tape Station Kit (Agilent 2200, 5067-5585 (reagents) #5067-5584 ( screen tapes)) for fragment length distribution and Qubit dsDNA HS assay kit (Invitrogen, Q32851) for quantifying the library.
description
Adenocarcinoma; Colorectal
disease
DZIP3 shRNA Knockdown
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
colon
organism part
Homo sapiens
scientific_name
SCD3HT29_KD2_S11
title